Reversed Phase PhyTip columns are used for automated desalting or cleanup of protein and peptide samples before mass spectrometry analysis. PhyTip columns perform this step with the consistency, ease and scalability that go hand-in-hand with automation. With the high capacity and by recovery in mass spectrometry compatible solvents, Reversed Phase PhyTip columns is the optimal choice in developing a fluid sample preparation workflow before mass spectrometry analysis. Reversed Phase PhyTip columns bind a wide range of protein and polypeptide sizes. The absolute binding capacity is high with a low loss of smaller or more polar polypeptides.
PhyNexus now part of Biotage developed the the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step for excellent protein purification results.