Purification of Peptides
When a peptide is synthesized and cleaved from a resin, the next step is usually a quick preparation and multiple injections onto a preparative HPLC column for isolation from deletion sequences and other contaminants. A great step to take before preparative HPLC is a single injection onto a flash purification system. This quick clean-up is often sufficient to move to the next step in the synthesis. Flash purification prior to preparative HPLC also creates the opportunity to further purify the peptide of interest with fewer injections and a less challenging mixture, and extends the life of expensive preparative HPLC columns.