Speed up peptide purification using flash chromatography

Purification is a key bottleneck in peptide synthesis workflows. Here are the benefits of high-performance flash chromatography in peptide workflows:

Handle larger sample sizes with higher loading capacity than RP-HPLC
Reduce waste and costs by using less solvents
Optimize your workflow with versatility for both primary purification and pre-RP-HPLC clean-up
Purify a wide range of peptides, including PNA, peptoids, and peptidomimetics

5 tips for successful peptide purification

Improve your peptide flash purification with practical tips for better results and faster workflows, plus expert guidance on optimizing gradients.

Faster peptide purification with reduced solvent use and lower costs

A study from the University of Copenhagen concludes that HPFC is a rapid, efficient alternative to RP-HPLC, enabling larger peptide loads per injection while significantly reducing purification time, solvent use, and costs.

Peptide workflow at Red Glead Discovery

Find out how Red Glead Discovery improves efficiency with flash purification. Some peptides achieve high purity in a single run, while others require additional RP-HPLC purification. The greatest time savings are seen with longer peptides.

Application note: Accelerate peptide purification with flash chromatography

Discover how flash chromatography can serve as the primary purification method for crude synthetic peptides, helping you speed up your workflow. Read Part 1 and Part 2.

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Flash systems for peptide purification

The Biotage® Selekt automated flash purification systems are optimized for fast, efficient peptide purification with a range of lab-scale columns with a choice of reversed-phase media.

Flash columns for peptide purification

Biotage® Sfär flash columns enable high-performance reversed-phase purification of peptides, developed with small particles and larger pores for efficient bulk purification or enrichment before RP-HPLC.

Biotage insights: Small molecules & synthetic therapeutics

Optimizing peptide purification with flash chromatography

A study shows flash chromatography efficiently purifies peptides, allowing larger loads per injection while saving time, solvent, and costs versus HPLC.

Does media choice matter for peptide library purification using solid phase extraction?

SPE is emerging as a strategy for peptide library clean up in parallel.

What is the difference between solid vs. liquid particles in ELSD signal?

ELSD is useful for finding molecules that have no chromophore during flash purification. Here we show how signal intensity can vary between compounds!

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Related products

Biotage® Selekt

A two column channel system with a colorful and intuitive user interface on a large touchscreen enables even the most complex tasks to become simplicity itself. High flowrate and pressure make separation faster and greener due to less solvent consumption.

Isolera™ Dalton 2000

The Isolera™ Dalton 2000 system consists of Biotage® Dalton 2000 Mass Detector, Isolera™ Dalton Nanolink and Isolera™ Spektra for efficient and automated purification. Isolera Dalton 2000 directs flash fractionation by molecular mass, and in real time.

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Biotage® Sfär Bio C4 D

High-performance flash chromatography is an efficient technique for purifying synthetic peptides. Biotage® Sfär Bio flash columns, with a small 20 μm particle size and large 300 Å pores, deliver high resolution and effective separation of complex peptide mixtures. For more hydrophobic peptides, C4 is the preferred choice.

Other steps in the workflow

Biotage offer a range of manual, semi-automated and fully automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple peptides in parallel, we have an instrument to suit your requirements.

Peptide synthesis

Peptide purification often generates a lot of fractions that require time-consuming concentration and pooling. Automated evaporation technologies can quickly and gently remove both aqueous and organic solvents, thus streamlining the process.

Evaporation of peptides