Protein A is the most widely used affinity media for antibody purification.
Both Protein A and Protein G bind to the Fc region of IgG antibodies and with various selectivity to IgG subtypes. As a result, Protein A and protein G PhyTip columns can be used for purification of IgG antibodies, and IgG fragments that include the Fc region.
Our Protein A PhyTip columns have a demonstrated ability to extract functional IgG antibodies with very high purity from varying levels of background proteins, due to their high binding affinity and high capacity.
Our Protein A PhyTip columns come with validated dual flow chromatography protocols for purification of IgGs that can be transferred to any automated liquid handling system. Our buffer kits contain all the buffers is needed for the purification protocol.
PhyNexus, now part of Biotage, developed the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.
Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries.
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in only 15 minutes.
When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.
We offer PhyTip® columns available for all major liquid handling systems. Download here our guide over PhyTip® column compatibility with different liquid handling systems.
If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
PTB-91-10-01 | Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 644 USD | Add to quote/cart |
PTX-91-10-01 | Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix. Incl. buffer kit. | 96 | 644 USD | Add to quote/cart |
PTH-91-10-01 | Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 644 USD | Add to quote/cart |
PTR-91-10-01 | Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 644 USD | Add to quote/cart |
PTT-91-10-01 | Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 644 USD | Add to quote/cart |
PTB-91-16-01 | Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 2204 USD | Add to quote/cart |
PTX-91-16-01 | Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix. Incl. buffer kit. | 96 | 2204 USD | Add to quote/cart |
PTH-91-16-01 | Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 2204 USD | Add to quote/cart |
PTR-91-16-01 | Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 2204 USD | Add to quote/cart |
PTT-91-16-01 | Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 2204 USD | Add to quote/cart |
PTB-91-20-01 | Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTX-91-20-01 | Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTH-91-20-01 | Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
17012568 | Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 371 USD | Add to quote/cart |
PTT-91-20-01 | Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTR-91-32-01 | Protein A PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 2542 USD | Add to quote/cart |
PTH-91-40-01 | Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 1045 USD | Add to quote/cart |
PTR-91-40-01 | Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 1045 USD | Add to quote/cart |
PTT-91-40-01 | Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 1045 USD | Add to quote/cart |
PTB-91-80-01 | Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 1656 USD | Add to quote/cart |
PTX-91-80-01 | Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix. Incl. buffer kit. | 96 | 1656 USD | Add to quote/cart |
PTH-91-80-01 | Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 1656 USD | Add to quote/cart |
17012569 | Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 829 USD | Add to quote/cart |
PTT-91-80-01 | Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 1656 USD | Add to quote/cart |
PTV-92-20-01 | Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTB-92-20-01 | Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTR-92-20-01 | Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
17012562 | Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 371 USD | Add to quote/cart |
PTT-92-20-01 | Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTB-92-05-01 | Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 526 USD | Add to quote/cart |
PTR-92-05-01 | Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 526 USD | Add to quote/cart |
17012561 | Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2. Incl. buffer kit. | 96 | 263 USD | Add to quote/cart |
PTT-92-05-01 | Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 526 USD | Add to quote/cart |
PTX-93-20-01 | Protein A PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTH-93-20-01 | Protein A PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTX-93-05-01 | Protein A PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix. Incl. buffer kit. | 96 | 526 USD | Add to quote/cart |
PTH-93-05-01 | Protein A PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton. Incl. buffer kit. | 96 | 526 USD | Add to quote/cart |
PTT-95-16-01 | Protein A PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 2204 USD | Add to quote/cart |
PTT-95-20-01 | Protein A PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 740 USD | Add to quote/cart |
PTT-95-40-01 | Protein A PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 1045 USD | Add to quote/cart |
PTT-95-80-01 | Protein A PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit. | 96 | 1656 USD | Add to quote/cart |
Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.
Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.
Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.
Biotage is headquartered in Uppsala in Sweden and employs over 500 people worldwide. The Group had sales of 1,566 MSEK in 2022 and our products are sold in more than 80 countries. Biotage’s share (BIOT) is listed in the Large Cap segment on the NASDAQ Stockholm.
Copyright © 2023 Biotage