Protein A PhyTip columns for automated purification of IgG antibodies

Protein A is the most widely used affinity media for antibody purification.

Both Protein A and Protein G bind to the Fc region of IgG antibodies and with various selectivity to IgG subtypes. As a result, Protein A and protein G PhyTip columns can be used for purification of IgG antibodies, and IgG fragments that include the Fc region.

Our Protein A PhyTip columns have a demonstrated ability to extract functional IgG antibodies with very high purity from varying levels of background proteins, due to their high binding affinity and high capacity.

Our Protein A PhyTip columns come with validated dual flow chromatography protocols for purification of IgGs that can be transferred to any automated liquid handling system. Our buffer kits contain all the buffers is needed for the purification protocol.

Benefits

PhyNexus, now part of Biotage, developed the PhyTip^® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.

Protein A PhyTip® Columns - Product Specification Sheet
A Novel Approach to Automated High Throughput Protein Enrichment and Characterization

All documents

70 - 90 % Recovery of IgGs

Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries.

Learn more

Sample Concentration and Purification in One

Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.

Learn more

IgG Purification in 15 min

Effective purification by PhyTip columns enables a full purification protocol of 96 samples in only 15 minutes.

Learn more

Validated Protocols

Our Protein A PhyTip columns comes with dual flow chromatography protocols that have been validated with real protein samples and can be transferred to any automated liquid handling system.

Learn more

Literature & Videos

PRODUCT PRESENTATION AND SPECIFICATION

Protein A PhyTip® Columns

BROCHURE

Biomolecule Purification with PhyTip® Columns

APPLICATION NOTE

Performance Characteristics and Assay Procedures for Protein A PhyTip® Columns

APPLICATION NOTE

A Novel Approach to Automated High Throughput Protein Enrichment and Characterization

APPLICATION NOTE

High Performance Immunoprecipitation-Direct Method

APPLICATION NOTE

High Performance Immunoprecipitation - Indirect method

WHITE PAPER

Accelerating High Throughput Protein Purification Using PhyTip® Columns

PDF

PhyTip® Columns Compatibility with Automation Systems Overview

SAFETY DATA SHEET

Protein A PhyTip Column

SAFETY DATA SHEET

Capture Buffer (A) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

SAFETY DATA SHEET

Wash 2 Buffer (P) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

SAFETY DATA SHEET

Elution Buffer (C) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

SAFETY DATA SHEET

Neutralization Buffer (G) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

Videos

PRODUCT VIDEO

Dual Flow Chromatography

This is how dual flow chromatography works - capture, wash, elute.

PRODUCT VIDEO

PhyTip Column Demonstration

Sample processing by PhyTip columns.

Part Numbers

When choosing a PhyTip^® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.

We offer PhyTip® columns available for all major liquid handling systems. Download here our guide over PhyTip^® column compatibility with different liquid handling systems.

If you are not sure which PhyTip^® column will that work best in your protocol, our specialists are here to help you.

Protein Amount Per Sample (Sample Concentrations >10 ug/mL)

<50 µg
50 - 100 µg
100 - 200 µg
200 - 400 µg
400 - 800 µg
800 - 1600 µg
1600 - 3100 µg

Automation System

MEA 2, ME
Hamilton
Beckman iSeries
Tecan

Agilent Bravo
Beckman FX & NX
Dynamic Devices Lynx
Dynamic Devices Oasis

Opentrons
Perkin Elmer SciClone, Calipher Zephyr, MiniTrack, Janus MTD
Janus Varispan, Cybio Felix

Part No.	Description	Pack Size	List Price
PTB-91-10-01	Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman. Incl. buffer kit.	96	644 USD	Add to quote/cart
PTX-91-10-01	Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	644 USD	Add to quote/cart
PTH-91-10-01	Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton. Incl. buffer kit.	96	644 USD	Add to quote/cart
PTR-91-10-01	Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2. Incl. buffer kit.	96	644 USD	Add to quote/cart
PTT-91-10-01	Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan. Incl. buffer kit.	96	644 USD	Add to quote/cart
PTB-91-16-01	Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman. Incl. buffer kit.	96	2204 USD	Add to quote/cart
PTX-91-16-01	Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	2204 USD	Add to quote/cart
PTH-91-16-01	Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton. Incl. buffer kit.	96	2204 USD	Add to quote/cart
PTR-91-16-01	Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2. Incl. buffer kit.	96	2204 USD	Add to quote/cart
PTT-91-16-01	Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit.	96	2204 USD	Add to quote/cart
PTB-91-20-01	Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTX-91-20-01	Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTH-91-20-01	Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit.	96	740 USD	Add to quote/cart
17012568	Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit.	96	371 USD	Add to quote/cart
PTT-91-20-01	Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTR-91-32-01	Protein A PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA 2. Incl. buffer kit.	96	2542 USD	Add to quote/cart
PTH-91-40-01	Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1045 USD	Add to quote/cart
PTR-91-40-01	Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA 2. Incl. buffer kit.	96	1045 USD	Add to quote/cart
PTT-91-40-01	Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit.	96	1045 USD	Add to quote/cart
PTB-91-80-01	Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman. Incl. buffer kit.	96	1656 USD	Add to quote/cart
PTX-91-80-01	Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	1656 USD	Add to quote/cart
PTH-91-80-01	Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1656 USD	Add to quote/cart
17012569	Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA 2. Incl. buffer kit.	96	829 USD	Add to quote/cart
PTT-91-80-01	Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit.	96	1656 USD	Add to quote/cart
PTV-92-20-01	Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTB-92-20-01	Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTR-92-20-01	Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit.	96	740 USD	Add to quote/cart
17012562	Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit.	96	371 USD	Add to quote/cart
PTT-92-20-01	Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTB-92-05-01	Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman. Incl. buffer kit.	96	526 USD	Add to quote/cart
PTR-92-05-01	Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2. Incl. buffer kit.	96	526 USD	Add to quote/cart
17012561	Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2. Incl. buffer kit.	96	263 USD	Add to quote/cart
PTT-92-05-01	Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan. Incl. buffer kit.	96	526 USD	Add to quote/cart
PTX-93-20-01	Protein A PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTH-93-20-01	Protein A PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTX-93-05-01	Protein A PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	526 USD	Add to quote/cart
PTH-93-05-01	Protein A PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton. Incl. buffer kit.	96	526 USD	Add to quote/cart
PTT-95-16-01	Protein A PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit.	96	2204 USD	Add to quote/cart
PTT-95-20-01	Protein A PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	740 USD	Add to quote/cart
PTT-95-40-01	Protein A PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit.	96	1045 USD	Add to quote/cart
PTT-95-80-01	Protein A PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit.	96	1656 USD	Add to quote/cart

Protein A PhyTip® Columns

Automated Purification of IgG Antibodies