The binding between streptavidin and biotin is the strongest non-covalent binding in nature and is commonly applied in biochemistry research. Our Streptavidin PhyTip columns packed with media with immobilized streptavidin affinity ligand enable automated affinity purification of recombinant biotin-tagged proteins. Our columns come with validated automated protocols for purification of biotin-tagged proteins that can be transferred to any automated liquid handling system.
One interesting application for Streptavidin PhyTip columns is biotinylated protein assays - immunoprecipitation where these column are used for immobilization of a biotin-tagged protein for generation of a customized affinity column.
PhyNexus now part of Biotage developed the the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.
PhyTip columns format is the perfect tool for parallel processing of samples in micro-scale in a high throughput process.
Our PhyTip columns are available for all major liquid handling systems, allowing their use on existing instrumentation you may already have in your lab!
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in little as 15 minutes.
When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.
We offer PhyTip® columns available for all major liquid handling systems.
If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
PTR-91-XX-05 | CUSTOM Box of 96 PhyTip® columns (1 mL volume) containing mixed volume of Streptavidin resin per column, MEA | 96 | 775 USD | Add to quote/cart |
PTB-91-10-05 | Streptavidin PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman | 96 | 790 USD | Add to quote/cart |
PTX-91-10-05 | Streptavidin PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix | 96 | 790 USD | Add to quote/cart |
PTH-91-10-05 | Streptavidin PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton | 96 | 790 USD | Add to quote/cart |
PTR-91-10-05 | Streptavidin PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2 | 96 | 790 USD | Add to quote/cart |
PTT-91-10-05 | Streptavidin PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan | 96 | 790 USD | Add to quote/cart |
PTB-91-16-05 | Streptavidin PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman | 96 | 4687 USD | Add to quote/cart |
PTX-91-16-05 | Streptavidin PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 4687 USD | Add to quote/cart |
PTH-91-16-05 | Streptavidin PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton | 96 | 4687 USD | Add to quote/cart |
PTR-91-16-05 | Streptavidin PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2 | 96 | 4687 USD | Add to quote/cart |
PTT-91-16-05 | Streptavidin PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan | 96 | 4687 USD | Add to quote/cart |
PTB-91-20-05 | Streptavidin PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman | 96 | 984 USD | Add to quote/cart |
PTX-91-20-05 | Streptavidin PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 984 USD | Add to quote/cart |
PTH-91-20-05 | Streptavidin PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton | 96 | 984 USD | Add to quote/cart |
PTR-91-20-05 | Streptavidin PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 984 USD | Add to quote/cart |
PTT-91-20-05 | Streptavidin PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan | 96 | 984 USD | Add to quote/cart |
PTR-91-32-05 | Streptavidin PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA 2 | 96 | 8812 USD | Add to quote/cart |
PTB-91-40-05 | Streptavidin PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman | 96 | 1603 USD | Add to quote/cart |
PTX-91-40-05 | Streptavidin PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1603 USD | Add to quote/cart |
PTH-91-40-05 | Streptavidin PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton | 96 | 1603 USD | Add to quote/cart |
PTR-91-40-05 | Streptavidin PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA 2 | 96 | 1603 USD | Add to quote/cart |
PTT-91-40-05 | Streptavidin PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan | 96 | 1603 USD | Add to quote/cart |
PTB-91-80-05 | Streptavidin PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman | 96 | 2747 USD | Add to quote/cart |
PTX-91-80-05 | Streptavidin PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 2747 USD | Add to quote/cart |
PTH-91-80-05 | Streptavidin PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton | 96 | 2747 USD | Add to quote/cart |
PTR-91-80-05 | Streptavidin PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA 2 | 96 | 2747 USD | Add to quote/cart |
PTT-91-80-05 | Streptavidin PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan | 96 | 2747 USD | Add to quote/cart |
PTV-92-20-05 | Streptavidin PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo | 96 | 984 USD | Add to quote/cart |
PTB-92-20-05 | Streptavidin PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman | 96 | 984 USD | Add to quote/cart |
PTR-92-20-05 | Streptavidin PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 984 USD | Add to quote/cart |
PTT-92-20-05 | Streptavidin PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 984 USD | Add to quote/cart |
PTB-92-05-05 | Streptavidin PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman | 96 | 681 USD | Add to quote/cart |
PTR-92-05-05 | Streptavidin PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2 | 96 | 681 USD | Add to quote/cart |
PTT-92-05-05 | Streptavidin PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan | 96 | 681 USD | Add to quote/cart |
PTX-93-20-05 | Streptavidin PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 984 USD | Add to quote/cart |
PTH-93-20-05 | Streptavidin PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton | 96 | 984 USD | Add to quote/cart |
PTX-93-05-05 | Streptavidin PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 681 USD | Add to quote/cart |
PTH-93-05-05 | Streptavidin PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton | 96 | 681 USD | Add to quote/cart |
PTT-95-16-05 | Streptavidin PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan | 96 | 4687 USD | Add to quote/cart |
PTT-95-20-05 | Streptavidin PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 984 USD | Add to quote/cart |
PTT-95-40-05 | Streptavidin PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan | 96 | 1603 USD | Add to quote/cart |
PTT-95-80-05 | Streptavidin PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan | 96 | 2747 USD | Add to quote/cart |
Biotage is the Global Go-To Separations Company, supporting customers from drug discovery and development through to diagnostics and analytical testing with intelligent and sustainable workflow solutions. Our expertise and top-tier separation solutions play a key role in streamlining our customers’ workflows and improving their outcomes. Headquartered in Sweden, Biotage operates globally with 700 employees, serving over 80 countries. Our company is listed on NASDAQ Stockholm (BIOT).
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