The binding between streptavidin and biotin is the strongest non-covalent binding in nature and is commonly applied in biochemistry research. Our Streptavidin PhyTip columns packed with media with immobilized streptavidin affinity ligand enable automated affinity purification of recombinant biotin-tagged proteins. Our columns come with validated automated protocols for purification of biotin-tagged proteins that can be transferred to any automated liquid handling system.
One interesting application for Streptavidin PhyTip columns is biotinylated protein assays - immunoprecipitation where these column are used for immobilization of a biotin-tagged protein for generation of a customized affinity column.
PhyNexus now part of Biotage developed the the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.