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    Gel Filtration PhyTip® Columns

    Automated High Throughput Desalting and Buffer Exchange

    Gel filtration PhyTip columns packed with size exclusion chromatography resin are ideal for desalting protein samples and for buffer exchange. Desalting by Gel Filtration PhyTip columns can be set up as a secondary purification step after affinity purification by affinity PhyTip columns in the same automated protocol, for example for imidazole removal after IMAC affinity purification.


    Just like other PhyTip columns, the resin is packed between two inert screens that maintain the resin bed at the base of the column. However, unlike other PhyTip columns, gel filtration columns are top loaded with sample; gravity flow pulls the sample through the gel. Gel filtration PhyTip columns are available with both 5K and 30K size exclusion resin that retain small molecules while molecules over 5 kDa and 30 kDa, respectively, passes through the column.

    PDR 91-20-17k

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      Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.

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      Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.

      Biotage is headquartered in Uppsala in Sweden and employs appr. 700 people worldwide. The Group had sales of 1,566 MSEK in 2022 and our products are sold in more than 80 countries. Biotage’s share (BIOT) is listed in the Large Cap segment on the NASDAQ Stockholm.

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      Gel filtration PhyTip columns for buffer exchange