Gel filtration PhyTip columns packed with size exclusion chromatography resin are ideal for desalting protein samples and for buffer exchange. Desalting by Gel Filtration PhyTip columns can be set up as a secondary purification step after affinity purification by affinity PhyTip columns in the same automated protocol, for example for imidazole removal after IMAC affinity purification.
Just like other PhyTip columns, the resin is packed between two inert screens that maintain the resin bed at the base of the column. However, unlike other PhyTip columns, gel filtration columns are top loaded with sample; gravity flow pulls the sample through the gel. Gel filtration PhyTip columns are available with both 5K and 30K size exclusion resin that retain small molecules while molecules over 5 kDa and 30 kDa, respectively, passes through the column.
Biotage Gel filtration PhyTip® columns empower researchers to prepare and process large numbers of potential antibody and protein leads by delivering a fully automated desalting and buffer exchange solution.
Gel Filtration PhyTip columns removes >95% of salts with >80% protein yield.
Sample desalting by Gel filtration PhyTip columns can easily be combined with affinity PhyTip columns for protein purification in the same automated workflow.
Our automated solution delivers highly consistent and reproducible results with <10% CVs of sample volumes and concentrations between purified samples, while maintaining protein functionality.
Gel filtration PhyTip columns can process up to 96 samples in about 30 min. Much faster than overnight dialysis for buffer exchange.
Our Gel Filtration PhyTip columns are compatible with the MEA 2 system or any other liquid handling system with a gripper arm. The gripper arm can be used to move the tray as necessary for desalting, buffer exchange or fractionation capture during screening of 96 samples in the same run.