Extraction of Catecholamines and Metanephrines from Human Plasma Biotage® Extrahera™ LV-200 and Low Volume SPE Prior to UHPLC-MS/MS Analysis

This application note describes the extraction of six catecholamines and metanephrines from human plasma using EVOLUTE® EXPRESS WCX 10 mg fixed well plates prior to UHPLC-MS/MS analysis. It includes optimized conditions for automated processing of the 10 mg plates (using Biotage® Extrahera LV-200, see appendix for settings) and manual processing (using the Biotage® PRESSURE+ 96 positive pressure manifold). Data generated using both processing systems is shown. Evaporation uses the TurboVap®96 Dual system.