Extraction of Three Phosphatidylethanol (PEth) Biomarkers in Human Whole Blood Using ISOLUTE® SLE+ Prior to LC/MS-MS Analysis

This application note describes the extraction of a panel of three phosphatidylethanol (PEth) alcohol biomarkers from human whole blood using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 60% with RSDs lower than 10% for all analytes, with reduced levels of phospholipids to improve extract cleanliness and analyte sensitivity. Linearity of greater than 0.999 is achieved for all analytes from 0.5-5000 ng/mL.