Fast Extraction of Metanephrine and Normetanephrine from Synthetic Urine Using EVOLUTE® EXPRESS WCX in a 96-Well Plate Format Prior to LC-ESI-MS/MS

This application note describes a method of extraction for metanephrine and normetanephrine from synthetic urine using EVOLUTE® EXPRESS WCX 96-well SPE plates. A new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach. This method was optimized for synthetic urine.