Method Optimization for the Low Level Detection of Vitamin B7 from Human Serum Using UPLC-MS/MS Analysis

Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize sensitivity allowing low level analysis of vitamin B7 from human serum. MSACL 2017, Palm Springs ASMS 2017, Indianapolis