Protein Purification Using PhyTip® Columns on the flowbot® ONE

Automating small-scale purification can enable more research, shorten workflows, free up time for other tasks, and standardize results. One effective way of automating protein purification is by utilizing PhyTip® columns, manufactured and sold by Biotage®, on a robotics platform.

In this application note, we will demonstrate our development of an automated protein purification solution using the flowbot® ONE* and PhyTip® columns. Specifically, we will present purification of human IgG samples using 1000 μL PhyTip® ProPlus with a 40 μL resin bed.