Sample Size Determination and Microelution with No Dry Down Using Mixed Mode Solid Phase Extraction for a Drugs of Abuse Panel

This poster explores the use of very low sample and elution volumes with 10 mg format EVOLUTE EXPRESS CX products for urine panel analysis. An extraction protocol using a 10 mg mixed-mode cation exchange sorbent (EVOLUTE® EXPRESS CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving microelution off of the sorbent requiring no evaporation or reconstitution steps were also developed. MSACL NA 2019