Scalable Flash Chromatography for Purifying Synthetic Lipids

 In this study, we demonstrate the purification of ALC-0315 from synthetic protocols using flash chromatography. By optimizing solvent composition, purification methods, stationary phase, and loading, we show that high-performance liquid chromatography (HPLC) is not always necessary for lipid purification. Our methodology offers a scalable approach to flash purification, suitable for multigram to kilogram scales.