Simplified Sample Preparation for Drugs of Abuse Extraction from Urine Samples Prior to LC-MS/MS Analysis

Dilute and shoot (D/S) is the most common form of sample preparation for the high throughput analysis of drugs of abuse (DoA)in urine. Although superior in extract cleanliness, solid phase extraction is sometimes deemed too complex, expensive and time consuming especially when matrix hydrolysis is required. This poster aims to demonstrate a simplified sample preparation workflow utilizing on-plate urine hydrolysis, organic solvent precipitation followed by flow through filtration and matrix component scavenging. ASMS, 2020.