Strategies Toward Optimizing Automated On-Resin Disulfide Bond Formation in Disulfide Rich Peptides

Herein we identified an optimized protocol for removing an Acm protecting group using the Biotage® Initiator+ Alstra™ and applied the protocol for a fully automated synthesis with on-resin disulfide bond formation, simplified with the Branches™ software feature. The results presented herein provide a route amenable to the synthesis of other disulfide rich peptides, greatly reducing the effort put toward synthesizing these complex molecules.