Food & Agriculture Sample Preparation

Acrylamide Analysis

Highlighted Applications

Still life of fresh roasted coffee beans with a white mug on a black slate surface

Automated extraction of Acrylamide using EN16618:2015

This application note describes the extraction of acrylamide from instant coffee according to EN16618:2015. The sample preparation procedure is automated using Biotage® Extrahera™ HV-5000 delivering clean extracts and analyte recoveries greater than 90% with RSDs lower than 10%.

Acrylamide in Coffee using SLE

The method described in this application note achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppb in ground coffee (solid) or 125 ppb in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.

Acrylamide in Crisps/Potato Chips using SLE

The method described in this application note achieves high recoveries of acrylamide in fried potato chips (crisps). The method is sensitive enough to measure levels as low as 10 ppb in a popular brand and flavor and has also been tested in flavored varieties that were both machine and hand fried.

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Recommended Products

ISOLUTE® SLE+

Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.

ISOLUTE® Multimode

Mixed-mode sorbent frequently used in 'scavenging' mode for isolation of compounds from complex mixtures. Non-polar, strong cation exchange and strong anion exchange retention mechanisms are combined together.

ISOLUTE® ENV+

The particle size and retentive nature of the sorbent allow exceptionally high sample flow rates to be used without reducing extraction efficiency or analyte recovery.

Positive Pressure Processing

Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns.

Extrahera™

Powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.

TurboVap® LV

Evaporation of up to 48 samples with independent control of flow in each row.

How do I come up with a starting point for my SPE method?

You've just decided to develop a solid phase extraction method for a new assay in your lab. However, there are so many decisions to be made! Which product to use? Polymer based sorbent or silica based? Cation or anion exchange? While this seems incredibly difficult, it doesn't have to be that hard!

Resources

Get Started with Supported Liquid Extraction

Method development chart, tips and tricks and useful insights to optimize your sample prep.

QuickStart Guide to SPE

The versatility of SPE allows it to be used for a variety of purposes.

Automating Sample Preparation. Why not?

Faster turnaround times and the need to reduce costs, and suddenly sample preparation isn’t quite so straightforward.

Need support? Contact us

Make your life easier. Simplify your workflow

Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.

Automation

Biotage evaporation systems are designed for fast solvent evaporation and minimizing sample transfer.