Catecholamines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine, epinephrine and norepinephrine which are traditionally analyzed using liquid chromatography with electrochemical detection.
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
EVOLUTE EXPRESS solid phase extraction products combine powerful EVOLUTE sorbent chemistry with enhanced ‘EXPRESS’ column components. EVOLUTE EXPRESS products dramatically improve flow characteristics, and enhance sample preparation productivity. By truly eliminating the need for column conditioning and equilibration, samples can be prepared using a simple, fast load-wash-elute procedure.
Ten Years with Environmental SPE Workflow - a User's Perspective
How St. John's University Accelerated their Cancer Research
Application Video: Extraction of Polyfluorinated Compounds in Water
Yes, You Can Add Biotage® Sfär Columns to Your Isolera™ System
Two New Applications Notes - Sample Preparation of a Drugs of Abuse Panel in Human Hair