EVOLUTE® HYDRO solid phase extraction columns and 96-well plates combine robust, high quality EVOLUTE® SPE sorbents with Hydro frit technology, enabling effective in-situ in-well hydrolysis of urine samples.
Sample clean-up is performed in the same column or well, without the need to transfer samples, saving time and reducing risky sample handling steps.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
600-0010-PZ01 | EVOLUTE® HYDRO ABN 10 mg Fixed Well Plate | 1 | 388 USD | Add to quote/cart |
600-0001-AZ | EVOLUTE® HYDRO ABN 10 mg/1 mL | 100 | 317 USD | Add to quote/cart |
600-0001-AZG | EVOLUTE® HYDRO ABN 10 mg/1 mL (Tabless) | 100 | 332 USD | Add to quote/cart |
600-0030-PZ01 | EVOLUTE® HYDRO ABN 30 mg Fixed Well Plate | 1 | 388 USD | Add to quote/cart |
600-0003-AZ | EVOLUTE® HYDRO ABN 30 mg/1 mL | 100 | 317 USD | Add to quote/cart |
600-0003-AZG | EVOLUTE® HYDRO ABN 30 mg/1 mL (Tabless) | 100 | 332 USD | Add to quote/cart |
601-0010-PZ01 | EVOLUTE® HYDRO CX 10 mg Fixed Well Plate | 1 | 395 USD | Add to quote/cart |
601-0001-AZ | EVOLUTE® HYDRO CX 10 mg/1 mL | 100 | 329 USD | Add to quote/cart |
601-0001-AZG | EVOLUTE® HYDRO CX 10 mg/1 mL (Tabless) | 100 | 346 USD | Add to quote/cart |
601-0030-PZ01 | EVOLUTE® HYDRO CX 30 mg Fixed Well Plate | 1 | 400 USD | Add to quote/cart |
601-0003-AZ | EVOLUTE® HYDRO CX 30 mg/1 mL | 100 | 329 USD | Add to quote/cart |
601-0003-AZG | EVOLUTE® HYDRO CX 30 mg/1 mL (Tabless) | 100 | 346 USD | Add to quote/cart |
With constant pressure to improve productivity and reduce repeats, without compromising on data quality, streamlining the sample preparation process has significant benefits.
The columns and 96-well plates contain the Hydro frit technology system, which reliably holds up sample and hydrolysis enzyme during incubation at elevated temperatures, and reduces clogging during subsequent clean up steps.
By combining Hydro frit technology with water wettable EVOLUTE sorbents, EVOLUTE HYDRO SPE products allow the hydrolyzed sample to be loaded directly onto the SPE bed for clean-up. Conditioning and equilibration steps are eliminated, again saving time and reducing costs.
Eliminating the need for off-line hydrolysis leads to significant time saving and reduction of sample handling, reducing potential errors and sample losses.
10 mg formats also allow analyte elution in very low volumes, allowing for no-blow down procedures.
Compared to traditional SPE procedures, EVOLUTE® HYDRO can reduce sample processing time by 20 minutes for 96 samples.
Automated extraction on Biotage® Extrahera™ using traditional SPE takes 53 minutes for 96 samples. With EVOLUTE® HYDRO this time is reduced to 33 minutes.
[30 mg 96-well plate format, using equivalent extraction methodology. Full procedure including hydrolysis, eluent evaporation and reconstitution: 143 mins (traditional SPE) 123 mins (HYDRO SPE)].
Because the HYDRO frit technology holds all of the sample above the sorbent bed during the hydrolysis process, hydrolysis efficiency when using EVOLUTE HYRO SPE columns and plates is equivalent to off-line hydrolysis under the same conditions. Existing hydrolysis procedures can easily be transferred to EVOLUTE HYDRO SPE products.
Whether extracting large multidrug panels or performing more targeted applications, EVOLUTE HYDRO SPE products remove matrix components and residual hydrolysis enzyme, leading to cleaner extracts and reduced matrix effects in LC-MS/MS.
Biotage is a global supplier and solutions partner to a wide range of customers within drug discovery and development and analytical testing. With strong expertise in separation and purification technologies, and a focus on leveraging intelligent workflow solutions, we aim to efficiently support the advancement and protection of human health.
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