Doping Control Sample Preparation - Human Blood & Urine Testing

Human Blood & Urine Testing

Highlighted Applications

Drugs of Abuse Panel from Whole Blood

This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. The protocol optimized the for 1 mL sample capacity column formats. The simple sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQ from 10 ng/mL.

Human Steroids in Serum

This application note describes the extraction of a panel of steroid hormones from human serum prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries for all analytes in the range 5–5000 pg/mL.

Steroid Panel in Urine

These methods describe simple approaches to extraction of a panel of endogenous steroids and metabolites from human urine samples.

The methods are easy to automate, and deliver high, reproducible recoveries, with excellent LOQs in the low pg/mL range for many analytes.

Supported Liquid Extraction (SLE) method
Solid Phase Extraction (SPE) method

Application Note Finder

Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!

Recommended Products

ISOLUTE® SLE+

Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.

EVOLUTE® EXPRESS ABN

Eliminate conditioning and equilibration with EVOLUTE® EXPRESS load-wash-elute SPE. Robust, reliable SPE, giving ultra-pure extracts and high recoveries. Specifically developed for quantification by LC-MS/MS.

ISOLUTE® HYDRO DME+

Extremely efficient removal of matrix components and hydrolysis enzyme from urine and other biological fluid samples, using a simple pass through workflow.

Positive Pressure Processing

Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns.

Extrahera™

Powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.

96 Well Plate Evaporators

Designed for high throughput laboratories, to provide efficient solvent evaporation in microplate formats.

Synthetic Benzodiazepines..There’s more than just Opioids to test for

We’ve all heard about Synthetic Opioids and how frequently they’re being abused, creating an Opioid Epidemic. What many of us aren’t hearing about are other synthetic compounds that are out there.

Resources

Get Started with Supported Liquid Extraction

Method development chart, tips and tricks and useful insights to optimize your sample prep.

Avoiding Cross Talk 96-well Based Sample Preparation

Eliminate carryover between samples, and reduce the risk of false positive results.

Watch the Webinar

Opioids: Choosing the Best Solution for Your Laboratory.

Need support? Contact us

Make your life easier. Simplify your workflow

Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.

Automation

Biotage evaporation systems are designed for fast sample evaporation and minimizing sample transfer.