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    Catecholamines & Metabolites

    Highlighted Applications

    Robust Extraction of Plasma Catecholamines and Metanephrines

    Optimized for clean-up of plasma samples containing a range of anticoagulants, giving robust performance for analysis to <10 pg/mL (<0.05 nmol/L) by LC-MS/MS analysis.

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    Automated Extraction of Urinary Catecholamines and Metanephrines

    A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable  for high throughput assays, the method can prepare 96 samples in 52 minutes.

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    Acidic Catecholamine Metabolites in Plasma (VMA, HVA, 5-HIAA)

    A simple ‘load-wait-elute’ supported liquid extraction methodology for the acidic metabolites, for LC-MS/MS analysis in the range of 5-200 mg/mL.

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    Automated Extraction of Plasma ‘Cats & Mets’ using Microelution SPE

    Microelution format methodology using mixed-mode weak cation exchange SPE, automated using the Biotage® Extrahera™ LV-200. This robust method can extract 96 samples in approximately 60 minutes.

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    This application is also available in 10 mg format.

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    Application Note Finder

    Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!

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    Resources

    Perfecting Pcats – Developing a More Robust UHPLC-MS/MS Method for Plasma Catecholamines using Low Volume SPE. 

    Selection of clinical sample preparation applications from Biotage.
    Eliminate carryover between samples, and reduce the risk of false positive results. 
    Make your life easier. Simplify your workflow

    Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.

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    Discover the full range of sample preparation products from Biotage.