Clinical & Bioanalytical Sample Preparation

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Highlighted Applications

Current Methodologies for Drugs of Abuse Urine Testing

This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Originally published in 2017, the white paper has now been updated to include details of additional hydrolysis enzymes and optimum hydrolysis conditions.

Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine

This application note describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sample clean up, resulting in clean extracts.

In-well Hydrolysis: Streamline Your Sample Handling

Save time and eliminate sample transfer steps, reducing the risk of errors and improving method robustness. This application note describes the extraction and clean up of 96 licit and illicit drugs using in-well hydrolysis of the urine sample.

Urine Hydrolysis: Does it matter how you do it?

Urine hydrolysis is performed to eliminate glucuronide drug metabolites before analysis, and it is key part of the sample preparation process. But it can involve extra sample transfer steps, and adds time to the sample preparation process. Streamline your workflow with integral in-well hydrolysis.

Application Note Finder

Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!

Recommended Products

ISOLUTE® SLE+

Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.

EVOLUTE® EXPRESS CX

Eliminate conditioning and equilibration with EVOLUTE® EXPRESS load-wash-elute SPE. Robust, reliable SPE, giving ultra-pure extracts and high recoveries. Specifically developed for quantification by LC-MS/MS.

ISOLUTE® HYDRO DME+

Efficient removal of matrix components and hydrolysis enzyme from urine and other biological fluid samples, using a simple pass through workflow. Available in columns and plates formats.

Positive Pressure Processing

Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns.

Extrahera™

Powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.

96 Well Plate Evaporators

Designed for high throughput laboratories, to provide efficient solvent evaporation in microplate formats.

How to extract and analyze for ever-present contaminants of interest?

It happens to all of us. We're getting a new method developed and validated and then it comes time to run our negative urines. And everything comes up as positive! There are peaks for our analytes of interest in every urine that we run! How is that possible?

Resources

Clinical Applications Compendium

Selection of clinical sample preparation applications from Biotage.

Get Started with Supported Liquid Extraction

Method development chart, tips and tricks and useful insights to optimize your sample prep.

Avoiding Cross Talk 96-well Based Sample Preparation

Eliminate carryover between samples, and reduce the risk of false positive results.

Watch the Webinar

Advances in Sample Prep Using In-well Hydrolysis.

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Make your life easier. Simplify your workflow

Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.