Steroids & Hormones

    Highlighted Applications

    steroid molecule structure

    Simple, Sensitive Extraction of 19 Human Steroids from Serum

    Extraction method of a comprehensive panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis.


    Automated Extraction of Urinary Catecholamines and Metanephrines

    A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable  for high throughput assays, the method can prepare 96 samples in 52 minutes.


    Asset 3@4x

    Simultaneous Extraction of Aldosterone and Angiotensin for PRA Analysis

    Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of high blood pressure.  This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs.


    Thyroid Hormones (T3, rT3 and T4) in Serum

    This extraction method using EVOLUTE® EXPRESS AX 96-well plates provides extremely clean, phospholipid and pigment free extracts which help maintain analytical system sensitivity. 



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