Simultaneous extraction of fat soluble vitamins (A, D, E and K) from serum presents a number of challenges, from the diversity of molecular structures and properties, to the wide variation in relevant clinical concentrations. Biotage specialists developed a method based on the Supported Liquid Extraction technique that effectively addresses these challenges.
Over the last 20 years, renewed scientific interest in the functions of Vitamin D in the body have required the development of sensitive methods for extraction and analysis of its various metabolites. Our specialists have developed the following application notes to deliver high analyte recoveries and clean extracts from serum.
Protocol for the extraction of 1,25 di-OH Vitamin D metabolites from serum using supported liquid extraction prior to LC-MS/MS detection.
Simple, effective protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.
Eliminate conditioning and equilibration with EVOLUTE® EXPRESS load-wash-elute SPE. Robust, reliable SPE, giving ultra-pure extracts and high recoveries. Specifically developed for analyte quantification by LC-MS/MS.
Protein & Phospholipid Removal products are effective sample clean-up for LC-MS/MS analysis. Easily integrated into routine workflow, increasing productivity and reducing instrument downtime.
Powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.
This blog post helps developing a method for the extraction of a panel of vitamins A, E and K.
Method development chart, tips and tricks and useful insights to optimize your sample prep.
Eliminate carryover between samples, and reduce the risk of false positive results.
Trials and Tribulations of Fat Soluble Vitamin Analysis by UHPLC-MS/MS.
Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.
Biotage evaporation systems are designed for fast sample evaporation and minimizing sample handling.
Discover the full range of sample preparation products from Biotage.