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Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma

15 October 2015

A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure, and combined with the very low elution volume possible with EVOLUTE EXPRESS 10 mg formats, it eliminates the need for an evaporation step – further reducing the time needed for sample processing.  LOQs of ~20pg/mL are achieved.

 

Catecholamines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine, epinephrine and norepinephrine which are traditionally analyzed using liquid chromatography with electrochemical detection.

This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.

EVOLUTE EXPRESS solid phase extraction products combine powerful EVOLUTE sorbent chemistry with enhanced ‘EXPRESS’ column components. EVOLUTE EXPRESS products dramatically improve flow characteristics, and enhance sample preparation productivity. By truly eliminating the need for column conditioning and equilibration, samples can be prepared using a simple, fast load-wash-elute procedure.

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