Catecholamines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine, epinephrine and norepinephrine which are traditionally analyzed using liquid chromatography with electrochemical detection.
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
EVOLUTE EXPRESS solid phase extraction products combine powerful EVOLUTE sorbent chemistry with enhanced ‘EXPRESS’ column components. EVOLUTE EXPRESS products dramatically improve flow characteristics, and enhance sample preparation productivity. By truly eliminating the need for column conditioning and equilibration, samples can be prepared using a simple, fast load-wash-elute procedure.
Achieve Highly Pure Peptides Using High Performance Flash Chromatography
White Paper from Biotage: Practical Considerations Using Quantisal® Oral Fluid Collection Devices
New UV Monitor Kit for Large Scale Purification
Peptide Workflow by Biotage
Thank you for visiting our Analytica Booth