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Extraction of Plasma Catecholamines and Metanephrines Using EVOLUTE® EXPRESS WCX SPE Prior to LC-MS/MS Analysis

05 July 2017

This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolites (metanephrine, normetanephrine and 3-methoxytyramine) from plasma prior to LC-MS/MS detection.

The method described in this application achieves high reproducible recoveries for a number of common catecholamine analytes in plasma. This method is easily automated using the Biotage®
Extrahera™. Details are included in the application note.

EVOLUTE® EXPRESS SPE products dramatically improve flow characteristics, and enhance sample preparation productivity. By truly eliminating the need for column conditioning and equilibration, samples can be prepared using a simple, fast load-wash-elute procedure.

Download the Application Note Below »


  • Epinephrine
  • Norepinephrine
  • Dopamine
  • Metanephrine
  • Normetanephrine
  • 3-Methoxytyramine (Sigma Aldrich Chemical Co, Poole, UK)

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