This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.
Tags: 6-acetyl codeine, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Application Notes, Aripiprazole, Atomoxetine, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Bupropion, Buspirone, Butalbital, Carbamate hypnotic, Carbamate muscle relaxant, Carbamates, Carbazepine, Carisoprodol, Chlordiazepoxide, Chlorpheniramine, Chlorpromazine, Clinical, Clomipramine, Clonazepam, Clonidine, Clozapine, Cocaethylene, Cocaine, Cocaines, Codeine, Cyclobenzaprine, Desalkylflurazepam, Designer stimulants, Dextromethorphan, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, Duloxetine, EDDP, English, Ethyl Sulfate (EtS), Extrahera, Fentanyl, Fluoxetine, Forensic, Gabapentin, Haloperidol, Hydrocodone, Hydrolysed urine, Hydromorphone, Hydroxyalprazolam, Hydroxybupropion, Hydroxymidazolam, Hydroxytriazolam, Imipramine, Ketamine, LC-MS/MS, Lamotrigine, Levetiracetam, Lidocaine, Lorazepam, MDA, MDEA, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Methaqualone, Methcathinone, Midazolam, Morphine, N-des-tapentadol, N-desmethylclomipramine, N-desmethyltapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Noroxymorphone, Norpropoxyphene, Nortryptiline, Opiates, Opioid, Opioid agonist, Oxazepam, Oxcarbazepine, Oxycodone, Oxymorphone, Pain Management, Pentobarbital, Pharmaceuticals, Phencyclidine (PCP), Phenobarbital, Pregabalin, Quetiapine, Ritalinic acid, Secobarbital, Stimulants, THC-COOH, Tapentadol, Temazepam, Topiramate, Tramadol, Triazolam, Trimipramine, Urine, Venlafaxine, Zolpidem, Zolpidem-phenyl-4-COOH, mCPP, z-drugs
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma
Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting.
This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation.
Tags: 96-well plate, Clinical, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Over the counter pharmaceuticals, Pharmaceuticals, Plasma, Posters, Urine
This poster presents a method for detection of approximately 100 drugs and metabolites in urine using mixed-mode polymeric cation exchange solid phase extraction (SPE).
Four different glucuronidase enzymes and several incubation times and temperatures were evaluated to determine optimum hydrolysis conditions for seven commonly detected glucuronide metabolites. Samples were subjected to offline hydrolysis and SPE (EVOLUTE® EXPRESS CX, Biotage, Charlotte, NC) and results compared to an SPE plate that incorporates in-well hydrolysis (EVOLUTE HYDRO CX, Biotage).
MSACL 2018, Palm Springs, CA
Tags: 96-well plate, Clinical, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Pain Management, Posters, Urine
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Tapentadol, Temazepam, Tramadol, Urine, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam
Most drugs, both licit and illicit, are excreted in urine as glucuronide conjugates. Hydrolysis using beta-glucuronidase converts the glucuronidated metabolites back to their “free” or non-conjugated
form, increasing sensitivity for LC-MS/MS analysis. Hydrolysis using red abalone, abalone, and recombinant beta-glucuronidase enzymes was evaluated using an in-well hydrolysis plate to determine which provided the most efficient hydrolysis of glucuronide metabolites without affecting overall recovery of nonconjugated compounds. A glucuronide control containing 8 glucuronide compounds was used to determine the extent of hydrolysis that occurred. A non-conjugated control containing 96 licit and illicit drugs of abuse was evaluated to determine if signal suppression occurred as a result of enzyme hydrolysis.
Tags: 96-well plate, Clinical, Codeine, Drugs of Abuse, English, Forensic, Hydrolysed urine, Hydromorphone, LC-MS/MS, Lorazepam, Morphine, Norbuprenorphine, Oxazepam, Oxymorphone, Pain Management, Posters, Urine
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
Tags: 96-well plate, Clinical, English, Oxytocin, Peptides, Pharma, Posters, Serum, Vasopressin
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
Tags: 96-well plate, Amikacin, Aminoglycoside antibiotics, Apramycin, Dihydrostreptomycin, Eggs, English, Extrahera, Food, Gentamicin, Gentamicin C1, Gentamicin C1a, Gentamicin C2, Hygromycin B, Kanamycin, LC-MS/MS, Meat, Milk, Neomycin, Netilmicin, Paromomycin, Posters, Sisomycin, Spectinomycin, Streptomycin, Tobramycin, Veterinary drugs
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12
Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldo...13 August 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...24 October 2017
This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...05 July 2017
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...29 May 2017
Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...19 May 2017
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...20 April 2017
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...15 October 2015
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...10 July 2014
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...30 June 2014
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/20 May 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...01 April 2014
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...11 January 2014
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...28 August 2012
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...28 August 2012
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...18 April 2011
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...16 December 2010
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...23 February 2010
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|600-0001-AZ||EVOLUTE® HYDRO ABN 10 mg/1 mL||Consumables||100||Log in for price|
|600-0001-AZG||EVOLUTE® HYDRO ABN 10 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|600-0003-AZ||EVOLUTE® HYDRO ABN 30 mg/1 mL||Consumables||100||Log in for price|
|600-0003-AZG||EVOLUTE® HYDRO ABN 30 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|600-0010-PZ01||EVOLUTE® HYDRO ABN 10 mg Fixed Well Plate||Consumables||1||Log in for price|
|600-0030-PZ01||EVOLUTE® HYDRO ABN 30 mg Fixed Well Plate||Consumables||1||Log in for price|
|601-0001-AZ||EVOLUTE® HYDRO CX 10 mg/1 mL||Consumables||100||Log in for price|
|601-0001-AZG||EVOLUTE® HYDRO CX 10 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|601-0003-AZ||EVOLUTE® HYDRO CX 30 mg/1 mL||Consumables||100||Log in for price|
|601-0003-AZG||EVOLUTE® HYDRO CX 30 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|601-0010-PZ01||EVOLUTE® HYDRO CX 10 mg Fixed Well Plate||Consumables||1||Log in for price|
|601-0030-PZ01||EVOLUTE® HYDRO CX 30 mg Fixed Well Plate||Consumables||1||Log in for price|
|610-0006-BZ||EVOLUTE® HYDRO ABN 60 mg/3 mL||Consumables||50||Log in for price|
||610-0006-BZG||EVOLUTE® HYDRO ABN 60 mg/3 mL (Tabless)||Consumables||50||Log in for price|
|610-0015-CZ||EVOLUTE® HYDRO ABN 150 mg/6 mL||Consumables||30||Log in for price|
||610-0015-CZG||EVOLUTE® HYDRO ABN 150 mg/6 mL (Tabless)||Consumables||30||Log in for price|
|611-0006-BZ||EVOLUTE® HYDRO CX 60 mg/3 mL||Consumables||50||Log in for price|
|611-0006-BZG||EVOLUTE® HYDRO CX 60 mg/3 mL (Tabless)||Consumables||50||Log in for price|
|611-0015-CZ||EVOLUTE® HYDRO CX 150 mg/6 mL||Consumables||30||Log in for price|
||611-0015-CZG||EVOLUTE® HYDRO CX 150 mg/6 mL (Tabless)||Consumables||30||Log in for price|