In this application note, we show that it is possible to completely eliminate a drifting baseline in UV detected purification by the use of advanced baseline correction features. Unlike many other systems, Isolera™ Spektra automatically organizes real blank runs for the chemist to create true background spectra and ensure maximum fidelity of the resulting baseline data, enabling almost any UV absorbing solvent combination to be used for flash purification.
Results of purification runs. System used: Isolera™ One running Spektra 2.0. UV-detector: 200–400 nm. Solvent A: Toluene; Solvent B: Acetone. Wavelengths used: 220 nm and 280 nm. Cartridge: Biotage® SNAP KP-Sil, 25 g. Sample: Nitronaphthalene, 2-Nitroaniline, 3-Nitroaniline (1.1:1 in acetone, 0.6 g/ml) 1 ml sample gives 2.4 % loading.
a) Example purification @ 265 and 280 nm detection [baseline correction off].
b) Example purification [λ-All: ON, baseline correction on].
New UV Monitor Kit for Large Scale Purification
Peptide Workflow by Biotage
Thank you for visiting our Analytica Booth
Application Video: Extraction Steroids from Serum with ISOLUTE® SLE+
Biotage Industrial Flash Cartridges Explained