Forensic & Toxicology Sample Prep - Preventing Cross Talk

Preventing Cross Talk

Highlighted Applications

Avoiding false positive results from sample to sample contamination is critical, and the use of 96-well plates may increase the risk due to close proximity of high concentration samples.

Evaporating ultra high concentration samples

Urine specimens analyzed by LC-MS/MS with analyte concentrations in excess of 100,000 ng/mL have been observed in some laboratories. Controlling crosstalk for these samples with such high concentrations presents a special challenge and is particularly difficult for volatile analytes like sympathomimetic amines.

This poster examines strategies to prevent cross talk for ultra high concentration samples.

Avoiding Cross Talk In 96-well Based Sample Preparation

This guide aims to describe simple practical strategies, hints and tips designed to mitigate or avoid cross-well contamination or ‘cross talk’ in high throughput 96-well based sample preparation techniques.

Application Note Finder

Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!

Recommended Products

Biotage® ACT Plate Adapter

Anti Cross Talk plate adapter is a novel solution to the phenomenon of hot spot cross contamination, or cross talk, during evaporation.

collection_plate_2_ml_square_pn_121-5203-a

ISOLUTE® Collection Plates

Deep Well Collection Plates. A range of square and round well collection plates. Constructed from high purity, solvent resistant polypropylene.

SPE Products

SPE columns, 96-well plates and disks: Polymeric, Non-polar, Polar, Ion exchange, Mixed-mode, Speciality media.

Positive Pressure Processing

Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns.

Extrahera™

Powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.

96 Well Plate Evaporators

Designed for high throughput laboratories, to provide efficient solvent evaporation in microplate formats.

What is Crosstalk and how do I Prevent it from Contaminating my Samples?

Evaporative crosstalk is contamination that occurs during evaporation of the elution solvent after SLE or SPE extraction. Volatile analytes evaporate, then redistribute in adjacent wells in a 96-well plate. This can generate false positive results and require re-extraction of patient specimens.

Resources

Get Started with Supported Liquid Extraction

Method development chart, tips and tricks and useful insights to optimize your sample prep.

Bioanalytical Sample Preparation - The Complete Guide

The pressure is always there to deliver accurate, reliable data, consistently and, if possible, at lower cost. Sample preparation is critical for success.

Watch the Webinar

Smarter Sample Prep: Your workflow from manual processing to automation.

Need support? Contact us

Make your life easier. Simplify your workflow

Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.

Automation

Biotage evaporation systems are designed for fast sample evaporation and minimizing sample handling.

Evaporation & Concentration

Discover the full range of sample preparation products from Biotage.

All Products for Sample Preparation