Ion exchange chromatography can be used either for a separate purification step or for a second polishing purification step after affinity chromatography purification as an orthogonal chemistry to obtain a highly pure final product. The result is complete protein capture and high final protein concentrations. Due to the physiological buffers used during the protocol, ion exchange chromatography is a generally gentle purification method.
Ion Exchange PhyTip® columns is used for efficient parallel purification of small protein samples.
PhyNexus now part of Biotage developed the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step for excellent protein purification results.