Normal phase PhyTip columns for mass spectrometry desalting

Sample processing by Normal Phase PhyTip columns by dual flow chromatography leads to specific enrichment of polar compounds – for instance oligosaccharides. As such, Normal Phase PhyTip columns are the only commercially available solution for high throughput, oligosaccharide purification for applications such as oligosaccharide mapping and identification analysis of glycoproteins.

Fluorescent dye labels are typically used to enhance detection of oligosaccharides performed by HPLC and capillary electrophoresis. Normal Phase PhyTip columns is enable automated sample dye removal before identification and quantification with high throughput and high reproducibility.

Benefits

PhyNexus, now part of Biotage developed the PhyTip^® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purifies samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step for excellent protein purification results.

All documents

Automated Oligosaccharide Purification in a High Throughput Format

Our PhyTip columns enable automation of processes that are otherwise difficult to automate. Normal phase PhyTip columns can be used for automated purification of oligosaccharide in a high throughput format.

Learn more

Sample Enrichment and Purification in One

Our unique column design with a packed resin bed results binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.

Learn more

Validated Protocols

Our normal phase PhyTip columns comes with dual flow chromatography protocols that have be validated with real protein samples and can be used on any automated liquid handling system.

Learn more

Speed

Normal phase PhyTip columns can be used for purification of 96 samples in as little as 15 minutes.

Learn more

Compatible with All Major Liquid Handling Platforms

Our PhyTip columns are available for all major liquid handling platforms to enable you to use your existing system that you may already have in your lab for automated protein purification.

Learn more

Part No.	Description	Pack Size	List Price
PTT-91-16-09	Normal Phase 1 PhyTip® columns (1 mL volume) containing 160 µL resin per column	96	3815 USD	Add to quote/cart
PTT-91-80-09	Normal Phase 1 PhyTip® columns (1 mL volume) containing 80 µL resin per column	96	2066 USD	Add to quote/cart
PTB-91-10-09	Normal phase I PhyTip® columns (1 mL volume) containing 10 µL silica per column, Beckman	96	775 USD	Add to quote/cart
PTX-91-10-09	Normal phase I PhyTip® columns (1 mL volume) containing 10 µL silica per column, DD/Biotix	96	775 USD	Add to quote/cart
PTH-91-10-09	Normal phase I PhyTip® columns (1 mL volume) containing 10 µL silica per column, Hamilton	96	775 USD	Add to quote/cart
PTR-91-10-09	Normal phase I PhyTip® columns (1 mL volume) containing 10 µL silica per column, MEA 2	96	775 USD	Add to quote/cart
PTT-91-10-09	Normal phase I PhyTip® columns (1 mL volume) containing 10 µL silica per column, Tecan	96	775 USD	Add to quote/cart
PTB-91-16-09	Normal phase I PhyTip® columns (1 mL volume) containing 160 µL silica per column, Beckman	96	4006 USD	Add to quote/cart
PTH-91-16-09	Normal phase I PhyTip® columns (1 mL volume) containing 160 µL silica per column, Hamilton	96	3815 USD	Add to quote/cart
PTR-91-16-09	Normal phase I PhyTip® columns (1 mL volume) containing 160 µL silica per column, MEA 2	96	4006 USD	Add to quote/cart
PTB-91-20-09	Normal phase I PhyTip® columns (1 mL volume) containing 20 µL silica per column, Beckman	96	916 USD	Add to quote/cart
PTX-91-20-09	Normal phase I PhyTip® columns (1 mL volume) containing 20 µL silica per column, DD/Biotix	96	916 USD	Add to quote/cart
PTH-91-20-09	Normal phase I PhyTip® columns (1 mL volume) containing 20 µL silica per column, Hamilton	96	916 USD	Add to quote/cart
PTR-91-20-09	Normal phase I PhyTip® columns (1 mL volume) containing 20 µL silica per column, MEA 2. Incl. buffer kit.	96	916 USD	Add to quote/cart
PTT-91-20-09	Normal phase I PhyTip® columns (1 mL volume) containing 20 µL silica per column, Tecan	96	916 USD	Add to quote/cart
PTB-91-40-09	Normal phase I PhyTip® columns (1 mL volume) containing 40 µL silica per column, Beckman	96	1368 USD	Add to quote/cart
PTB-91-80-09	Normal phase I PhyTip® columns (1 mL volume) containing 80 µL silica per column, Beckman	96	2169 USD	Add to quote/cart
PTH-91-80-09	Normal phase I PhyTip® columns (1 mL volume) containing 80 µL silica per column, Hamilton	96	2066 USD	Add to quote/cart
PTV-92-20-09	Normal phase I PhyTip® columns (200 µL volume) containing 20 µL silica per column, Agilent Bravo	96	916 USD	Add to quote/cart
PTB-92-20-09	Normal phase I PhyTip® columns (200 µL volume) containing 20 µL silica per column, Beckman	96	916 USD	Add to quote/cart
PTR-92-20-09	Normal phase I PhyTip® columns (200 µL volume) containing 20 µL silica per column, MEA 2	96	916 USD	Add to quote/cart
PTT-92-20-09	Normal phase I PhyTip® columns (200 µL volume) containing 20 µL silica per column, Tecan	96	916 USD	Add to quote/cart
PTB-92-05-09	Normal phase I PhyTip® columns (200 µL volume) containing 5 µL silica per column, Beckman	96	546 USD	Add to quote/cart
PTR-92-05-09	Normal phase I PhyTip® columns (200 µL volume) containing 5 µL silica per column, MEA 2	96	546 USD	Add to quote/cart
PTT-92-05-09	Normal phase I PhyTip® columns (200 µL volume) containing 5 µL silica per column, Tecan	96	546 USD	Add to quote/cart
PTX-93-20-09	Normal phase I PhyTip® columns (300 µL volume) containing 20 µL silica per column, DD/Biotix	96	916 USD	Add to quote/cart
PTH-93-20-09	Normal phase I PhyTip® columns (300 µL volume) containing 20 µL silica per column, Hamilton	96	916 USD	Add to quote/cart
PTX-93-05-09	Normal phase I PhyTip® columns (300 µL volume) containing 5 µL silica per column, DD/Biotix	96	546 USD	Add to quote/cart
PTH-93-05-09	Normal phase I PhyTip® columns (300 µL volume) containing 5 µL silica per column, Hamilton	96	546 USD	Add to quote/cart
PTT-95-16-09	Normal phase I PhyTip® columns (500 µL volume) containing 160 µL silica per column, Tecan	96	4006 USD	Add to quote/cart
PTT-95-20-09	Normal phase I PhyTip® columns (500 µL volume) containing 20 µL silica per column, Tecan	96	916 USD	Add to quote/cart
PTT-95-40-09	Normal phase I PhyTip® columns (500 µL volume) containing 40 µL silica per column, Tecan	96	1368 USD	Add to quote/cart
PTT-95-80-09	Normal phase I PhyTip® columns (500 µL volume) containing 80 µL silica per column, Tecan	96	2169 USD	Add to quote/cart

Normal Phase PhyTip® Columns

Glycan Enrichment for Oligosaccharide Mapping and Identification by Normal Phase PhyTip Columns

Benefits

Automated Oligosaccharide Purification in a High Throughput Format

Sample Enrichment and Purification in One

Validated Protocols

Speed

Compatible with All Major Liquid Handling Platforms

Literature

Videos

Dual Flow Chromatography

PhyTip Column Demonstration

Part Numbers

About Biotage

Ask a specialist

Normal Phase PhyTip® Columns

Glycan Enrichment for Oligosaccharide Mapping and Identification by Normal Phase PhyTip Columns

Benefits

Automated Oligosaccharide Purification in a High Throughput Format

Sample Enrichment and Purification in One

Validated Protocols

Speed

Compatible with All Major Liquid Handling Platforms

Literature

Videos

Dual Flow Chromatography

PhyTip Column Demonstration

Part Numbers

Your Cart

Related products

Antibody Purification

Tagged Protein Purification

Gel Filtration PhyTip® Columns

Automation Systems

About Biotage

Ask a specialist