Part No: Issued year: 2010File size: 1.9mbFile type: pdf
User Report: V-10, Sumitomo Dainippon Pharma. Sumitomo Dainippon Pharma is recognized worldwide for its highly creative drug discovery program for treating neuropsychiatric disorders, and in specialized areas with high unmet medical needs. Its Genomic Science Laboratories installed the V-10 evaporation system in 2008, which has been used extensively for drying in-house compounds. We asked two researchers at that facility, Mr. Masahiko Ikeda and Mr. Fumitaka Nishino, about their use of the V-10 in their daily work.
Part No: Issued year: 2005File size: 0.57mbFile type: pdf
Use of the unique opportunities of an academic environment for the synthesis of high-quality libraries of diverse chemical structures to discover small molecules with
architecturally novel scaffolds and a wide range of physicochemical properties and to develop innovative new methodologies for Diversity-Oriented Organic Synthesis
Part No: TN115Issued year: 2006File size: 0.08mbFile type: pdf
This Chemistry Data Sheet describes the use of ISOLUTE® HM-N as a support material for pre-absorption of a
reaction mixture prior to flash chromatography. This approach is used as a solution to loading reaction
mixtures that are only soluble in polar solvents onto an ISOLUTE Flash SI II or Flash NH2 column.
Part No: TN118Issued year: 2006File size: 0.07mbFile type: pdf
ISOLUTE HM-N is a modified form of diatomaceous earth that can efficiently absorb aqueous samples. ISOLUTE HM_N is chemically inert and stable in the pH range 1-13. These characteristics make it a versabile material that plays an important role in many sample preparation applications.
Part No: Issued year: 2006File size: 0.83mbFile type: pdf
• Novo Nordisk A/S & radiochemistry at Novo Nordisk A/S
• Introduction to radiochemistry in general
• Radiochemistry & microwaves
• Selected case stories from the labs at Novo Nordisk A/S
• Applications of radiolabelled compounds
• Summary and acknowledgements
Part No: P016Issued year: 2007File size: 0.16mbFile type: pdf
In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.
Part No: PPS433Issued year: 2016File size: 0.78mbFile type: pdf
Customer Case: Japan Frozen Foods Inspection Corporation.
The Japan Frozen Foods Inspection Corporation (JFFIC) made the transition from traditional mouse
testing of shellfish toxins, to instrumental analysis by developing original methods. In the sample
preparation procedure JFFIC uses EVOLUTE® ABN hydrophilic/hydrophobic SPE columns from Biotage.
Part No: PPS426Issued year: 2016File size: 0.38mbFile type: pdf
Customer case: Welsh Water. Analytical on-line cartridges for environmental testing from Biotage were released in 2016. Russell Gibbs from Dŵr Cymru Welsh Water has just started using them for drinking water testing.
Part No: CM-TRIT-0810Issued year: 2010File size: 0.26mbFile type: pdf
Trityl-ChemMatrix is mainly used to obtain a protected peptide as is has very low TFA cleavage conditions (under 1%). This resin therefore has the same cleavage conditions as the Cl-Trityl-polystyrene. On request, Trityl-ChemMatrix resins can
be offered preloaded will all 20 natural amino acids. Other amino acids can be preloaded on special request. Please take note that the Cl-Trityl-ChemMatrix resin is not offered for stability reasons. It is most effective to start with the Trityl-
OH-ChemMatrix resinchlorinate the resin and then add the amino acid of desire.
Part No: PPS448.v2Issued year: 2017File size: 0.53mbFile type: pdf
The TurboVap® 96 is a high speed concentrator designed to work with 96-well microplates and deep-well plates. It is an efficient alternative to the constant monitoring and long evaporation times that are characteristic of conventional techniques — with the added bonus of unattended operation.
Part No: TV-SS-07Issued year: 2010File size: 0.23mbFile type: pdf
In order to decide which model TurboVap will best fit a laboratory’s application, the following questions should be asked, along with following the flowchart on the reverse side:
• After extraction on the Dionex ASE, what is the matrix of my extract?
• After extraction on the Dionex ASE, will the extract require further cleanups or drying steps?
• What other types of samples may require concentration that are not extracted on the ASE, so that I may select the model TurboVap that will be suitable for all types?
• Do I have enough Dionex ASE extracts and enough non-ASE extracts to justify two separate model TurboVaps?
• Does it make sense for me to purchase a standard TurboVap model now, and convert with the ASE compatible kit later?
Part No: TV-SS-05Issued year: 2010File size: 0.24mbFile type: pdf
In order to concentrate to 1.0 mL of solvent, the TurboVap must be configured such that the sensor is mounted with spacers on the bottom plate of the internal rack (Figure 1). To concentrate to 0.5 mL of solvent, the TurboVap must be configured such that the sensor is flush to the bottom plate of the internal rack (Figure 3). If the TurboVap is not correctly configured, the light beam from the sensor will not be aligned with the desired endpoint of the tube.
Part No: UI324Issued year: 2015File size: 0.75mbFile type: pdf
In determining the gas flow rate, the goal is to achieve
the maximum evaporation rate possible without
splashing or compromising the sample. During
evaporation the distance between the nozzle tips
and the liquid surface increases, therefore, the flow
rate may be safely increased as the evaporation
progresses to shorten evaporation time, if so desired.
Part No: PPS438.v6Issued year: 2018File size: 1.43mbFile type: pdf
TurboVap® is a second generation product which builds on the solid
foundations of the historic TurboVap product line and incorporates many new
customer-driven features. It still features the highly efficient patented gas
vortex shearing technology, which is synonymous with the TurboVap brand.
Part No: AN857Issued year: 2016File size: 2.29mbFile type: pdf
This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection.
A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Part No: Issued year: 2011File size: 0.16mbFile type: pdf
Molecularly Imprinted Polymers (MIPs) are highly cross-linked polymers with selective binding sites engineered to contain recognition elements in defined positions1. MIPs have been developed for a large variety of small molecules, peptides, carbohydrates and even proteins. The schematic 0of a MIP site.
Part No: P055Issued year: 2013File size: 0.7mbFile type: pdf
Free urinary cortisol levels can be an indicator of a variety of disorders, such as Cushing Syndrome. This poster presents a simple method for the extraction of cortisol from urine demonstrating good recoveries and low ion suppression. LC-MS/MS analysis was performed using a Waters 2795 Liquid Handling Unit coupled to a Quattro Ultima Pt triple quadrupole MS using electrospray ionization, operated in the MRM mode. Supported liquid extraction method development resulted in recoveries greater than 90% for cortisol spiked urine. Calibration curves constructed using this method from 25-2000 ng/mL, demonstrated excellent linearity with coefficients of determination greater than 0.99.
Cortisol, Stress, Cushin's Syndrome, SLE, Supported Liquid Extraction, SPE, LLEMSACL, San Diego, 2013
Part No: Issued year: 2004File size: 0.39mbFile type: pdf
•Metal-mediated [2 + 3] cycloaddition to coordinated nitriles
-- synthesis of new transition metal complexes
-- synthesis of new heterocycles
-- enantioselective synthesis of heterocycles
role of microwaves?
• Platinum-catalysed cycloisomerisation of alkynes
-- Synthesis of Taxol-like compounds
-- highly efficient and atom economic chemistry
role of microwaves?
Part No: AN061Issued year: 2012File size: 0.63mbFile type: pdf
Acetone is successfully used as solvent in normal-phase flash chromatography when used with an Isolera™ Spektra flash purification system. The new λ-All detection and baseline correction features provide compound detection at all wavelengths in the detector’s range while minimizing any baseline drift due to solvent UV absorption.
Part No: PPS410Issued year: 2015File size: 0.7mbFile type: pdf
User Case: Professor Roger Strömberg, Karolinska Institutet. One of Professor Strömberg’s projects at Karolinska Institutet
involves the synthesis of artificial nucleases and PNAzymes. In
2013, his research group acquired a Biotage® Initiator+ Alstra™
peptide synthesizer for making PNAs and peptides.
Part No: PPS409Issued year: 2015File size: 1.61mbFile type: pdf
RIKEN is Japan’s largest research organization with 3,400
researchers from more than 50 countries. The chemists in RIKEN’s
Sugiyama Laboratory use EVOLUTE® EXPRESS solid-phase extraction
plates from Biotage in the analysis of clinical trial samples
for pharmacokinetic studies.