This application note describes the extraction of buprenorphine and norbuprenorphine from whole blood using ISOLUTE® SLE+ Supported Liquid Extraction prior to LC-MS/MS analysis.

96-well plate Application Notes Buprenorphine Clinical English Forensic LC-MS/MS Norbuprenorphine Pain Management Whole blood

Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) are biomarkers for neuroblastoma and catecholaminesecreting tumors. This poster describes optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Parameters investigated include MRM transitions, chromatography and sample preparation protocols. MSACL EU 2017

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Catecholamines Clinical English Homovanillic acid (HVA) LC-MS/MS Plasma Posters Vanillylmandelic acid (VMA)

Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5 HIAA) are biomarkers for neuroblastoma and catecholamine-secreting tumors. This poster presents optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Sample preparation protocols using solid phase extraction (EVOLUTE EXPRESS ABN and AX) and supported liquid extraction (ISOLUTE SLE+) are presented. MSACL NA 2018 Palm Springs, CA

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Catecholamines Clinical English Forensic Homovanillic acid (HVA) LC-MS/MS Plasma Posters Vanillylmandelic acid (VMA)

This method details the use of a non-polar retention mechanism for the extraction of catecholamines (noradrenaline, adrenaline, dopamine) from urine. The polar analytes form a non-polar complex with phenylboronic acid and are retained on the non-polar MFC18 column. Typical recoveries are >85%. ISOLUTE, Catecholamines, dopind, sports drug testing,

Adrenaline Application Notes Catecholamines Clinical Column Dopamine HPLC Noradrenaline Urine

The following methods have been developed and optimized for the extraction of chloramphenicol from a variety of sample matrixes including milk, plasma, honey, urine, and shrimp/prawns for subsequent LC-MS/MS analysis. The methods are highly reproducible and offer low limits of detection.

Antibiotics Application Notes Chloramphenicol Column Food Honey LC-MS/MS Milk Plasma Prawns Shellfish Shrimps Urine Veterinary drugs

The following methods have been developed for the selective extraction of clenbuterol from calf urine. The method is highly reproducible and offers clenbuterol recoveries of ≥ 70%. MIP, Molecularly Imprinted Polymers, AFFINILUTE, SupelMIP,

Application Notes Bronchodilator Clenbuterol Clinical Column HPLC Urine Veterinary drugs

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample (hydrolysed urine) volumes of either 200 μL or 1 mL. Procedures for high throughput (96-well plate) and column format are included. The simple sample preparation procedure delivers clean extracts and analyte recoveries of 82-100% with RSDs of <5% for all analytes. Sub ng/mL LOQs can be achieved.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Butylone Cocaines Column Ecgonine Methyl Ester EME Forensic Hydrolysed urine LC-MS/MS Norcocaine

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample (urine) volumes of either 200 μL or 1 mL. Procedures for high throughput (96-well plate) and column format are included. The simple sample preparation procedure delivers clean extracts and analyte recoveries of 67-100% with RSDs of <5% for all analytes. Sub ng/mL LOQs can be achieved.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Butylone Cocaines Column Ecgonine Methyl Ester EME Forensic LC-MS/MS Norcocaine Urine

This procedure is recommended for the extraction of cocaine and its principle metabolites (norcocaine, benzoylecgonine, ecgonine methyl ester, anhydroecgonine methyl ester and cocaethylene) from whole blood, plasma and urine using polymeric mixed-mode cation exchange SPE columns.

Anhydroecgonine methyl ester Application Notes Benzoylecgonine Biological fluids Butylone Cocaines Column Ecgonine methyl ester Forensic LC-MS/MS Norcocaine

This application note describes effective and efficient protocols for extraction of cocaine and its major metabolites optimized for sample loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <7% for all analytes.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Hydrolysed urine Norcocaine

This application note describes the extraction of cocaine and selected metabolites from oral fluid matrix following the collection with the NeoSalTM device, prior to GC/MS analysis.

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME Ecgonine methyl ester English Forensic GC-MS Norcocaine Oral fluid Saliva

This application note describes effective and efficient protocols optimized for loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs of <10% for all analytes.

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME Ecgonine methyl ester English Forensic GC-MS Norcocaine Oral fluid

This application note describes effective and efficient protocols optimized for sample volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs of <10% for all analytes.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Norcocaine Urine

This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Clinical Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Whole blood

This application note describes the extraction of cocaine, benzoylecgonine and cocaethylene from whole blood and human tissue using ISOLUTE HCX columns.

Application Notes Benzoylecgonine Butylone Cocaines Column Forensic LC-MS/MS Post-Mortem tissue Whole blood

This Application Note highlights the use of ISOLUTE® SLE+ supported-liquid extraction plates for the extraction of corticosteroids from human plasma, specifically Triamcinolone, Prednisolone, Hydrocortisone, Prednisone, Betamethasone, Cortisone, Dexamethasone, Flumethasone, Corticosterone, Beclomethasone, Triamcinolone, Fluocinolone, Budesonide 1, Budesonide 2 and 5-Pregnen-3β−ol-20-one.

5-pregnen-3β-ol-20-one Application Notes Beclomethasone Betamethasone Budesonide Structural Isomer 1 Budesonide Structural Isomer 2 Clinical Corticosteroids Corticosterone Cortisone Dexamethasone Flumethasone Fluocinolone Acetonide Hydrocortisone LC-MS/MS Plasma Prednisolone Prednisone Triamcinolone Triamcinolone Acetonide

Naturally occurring corticosteroids are steroid hormones produced in the adrenal cortex and perform many functions within the body. Due to their widespread action many synthetic corticosteroids based on the hydrocortisone (cortisol) structure have been produced. Some of their uses involve treatment of various forms of arthritis, dermatitis, hepatitis and ulcerative colitis. They can be administered orally, injected into veins or muscle, nasal sprays and drops or used as topical creams. Reliable screening methods for these drugs are important as they are classed as ‘banned substances’ by many anti-doping agencies and regulated for use in meat producing animals.

Posters

This application note describes a solid phase extraction (SPE) protocol for the extraction of deoxynivalenol (DON) from wheat, maize and barley using ISOLUTE® Myco SPE columns with LC-MS/MS analysis.

Application Notes Barley Column DON Deoxynivalenol (DON) Food LC-MS/MS Maize Mycotoxin Wheat

This application note describes the extraction of a range of recreational ecstasy-like drugs, designer stimulants and the hallucinogen 2C-B from urine prior to GC/MS analysis. The method described in this application note may also be suitable for extraction of other related analogs and derivatives of these drug compounds, for example the substituted cathinone class (bathsalts). This application note is optimized for extraction of 1 mL sample volumes.

2C-B 5-APB 6-APB Amphetamine Application Notes BZP Column Designer stimulants Drugs of Abuse Forensic GC-MS TFMPP Urine

This application note describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. The method uses EVOLUTE(R) EXPRESS WCX 96-well plates to achieve high recoveries with minimal matrix effects.

96-well plate Application Notes Clinical Desmopressin English LC-MS/MS Peptides Serum