This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of a range of mycotoxins from cereal based infant food using ISOLUTE® Myco with UHPLC-MS/MS. The method achieves high recoveries of relevant mycotoxins from a cereal based infantfood with low %RSDs and LOQs.

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Column English Food Fumonisin B1 HT-2 mycotoxin Infant food (cereal) LC-MS/MS Mycotoxin Ochratoxin A T-2 mycotoxin Zearalenone

This application note describes a solid phase extraction (SPE) protocol for the extraction of a range of mycotoxins from grain (wheat, barley, maize, wheat flour) using ISOLUTE Myco SPE columns for LC-MS/MS analysis. The method described achieves high recoveries of relevant mycotoxin classes from a range of different grain matrices with RSDs and LOQs that meet the requirements of EU regulations. mycotoxins, grains, LC-MS/MS, food safety

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Barley Column Ergocornine Ergocryptine Food Fumonisin B1 Grain HT-2 mycotoxin LC-MS/MS Maize Mycotoxin Ochratoxin A T-2 mycotoxin Wheat Wheat flour Zearalenone

This application note describes the extraction of mycotoxins (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A) from nuts (brazil nut, groundnut (peanut) using ISOLUTE Myco SPE columns with LC-MS/MS analysis

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Bovine kidney Clinical Column Food Groundnut (Peanut) LC-MS/MS Mycotoxin Nuts Ochratoxin A

This application note describes the extraction of the immunosuppressant drug mycophenolic acid and its metabolite mycophenolic acid glucuronide from fortified serum using ISOLUTE SLE+ in a 96-well plate format.

96-well plate Acidic Application Notes Clinical English Immunosuppressant LC-MS/MS Mycophenolic Acid Mycophenolic Acid Glucuronide Serum

Immunosuppressant drugs are instrumental in preventing organ and tissue rejection in patients undergoing transplant surgery. Mycophenolic Acid is a common immunosuppressant drug used in patient transplant therapy. The ability to monitor the trough levels in patients to evaluate dosing is important for the administering of the drug. The free drug and its glucuronidated metabolite can be found in patient serum. The ability to extract the free drug and metabolite in patient serum is supported by using a fast and efficient sample preparation extraction method called Supported Liquid Extraction (ISOLUTE SLE+). The free drug and metabolite can be recovered from serum using ISOLUTE SLE+ with high enough efficiency to allow for quantitation at target trough levels. MSACL 2014

96-well plate Clinical English Immunosuppressant LC-MS/MS Mycophenolic Acid Mycophenolic Acid Glucuronide Posters Serum

This poster describes the extraction of the commonly used immunosuppressant drug mycophenolic acid and its glucuronide metabolite from serum using ISOLUTE SLE+ supported liquid extraction plates. ASMS 2014

96-well plate Clinical Immunosuppressant LC-MS/MS Mycophenolic Acid Mycophenolic Acid Glucuronide Posters Serum

This application note describes the extraction of a suite of NBOMe designer drugs from oral fluid matrix with analysis by laser diode thermal desorption (LDTD)-MS/MS.

25B-NBOMe 25C-NBOMe 25D-NBOMe 25E-NBOMe 25H-NBOMe 25N-NBOMe 25T2-NBOMe Application Notes Column Drugs of Abuse English Forensic LDTD-MS/MS Legal highs NBOMes Oral fluid Saliva

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of nicotine, cotinine, 3-OH-cotinine, nornicotine, norcotinine and anabasine from urine, serum, plasma and whole blood using ISOLUTE® SLE+ plates with LC-MS/MS detection. nicotine cotinine nornicotine norcotinine 3-OH cotinine anabasine smoker

3-OH-cotinine Anabasine Application Notes Clinical Cotinine LC-MS/MS Nicotine Nicotine metabolites Norcotinine Nornicotine Plasma Serum Urine Whole blood

Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase.

Application Notes Column DMZOH Dimetridazole (DMZ) Food IPZOH Ipronidazole (IPZ) LC-MS/MS MNZOH Metronidazole (MNZ) Milk Nitroimidazoles Ronidazole Veterinary drugs Water

This Application Note highlights the use of supported-liquid Extraction to isolate three common NSAIDs, Sulindac, Flurbiprofen and Ibuprofen, from human plasma. Biotage, Ostro, Liquid liquid extraction, supported liquid extraction, Extrelute

Application Notes Clinical Flurbiprofen Ibuprofen LC-MS/MS NSAIDs Plasma Sulindac

Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase. molecularly imprinted polymers, MIP, BIOTAGE, AFFINILUTE, NSAIDs, Environmental, SupelMIP

Application Notes Clinical Clofibric Acid Column Ibuprofen LC-MS/MS NSAIDs Naproxen Post-Mortem fluids Post-Mortem tissue Water

This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP)) from human urine. This simplified and efficient extraction method has significant analyte recoveries ranging from 70-102% with LODs as low as 500 pg/mL. Opiates, SLE, SLE+, Supported Liquid Extraction, Urine, Oasis, Waters, SPE, Solid Phase Extraction, Sample Prep, quick, easy, DOA, Drugs of Abuse

6-acetylmorphine (MAM) Application Notes Codeine Column Dihydrocodeine (DHC) EDDP Forensic Hydrocodone Hydromorphone LC-MS/MS Methadone Morphine Opiates Oxycodone Oxymorphone Urine

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of opiate compounds from oral fluid matrix collected using the NeoSal device, prior to GC/MS analysis.

6-acetylmorphine (MAM) Application Notes Codeine Column Dihydrocodeine (DHC) Drugs of Abuse English Forensic GC-MS Hydrocodone Hydromorphone Morphine Opiates Oral fluid Oxycodone Oxymorphone Saliva

This application note describes the extraction of opiate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and cocaine. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 50 ng/mL (analyte dependant).

6-acetylmorphine (MAM) Application Notes Clinical Codeine Column Dihydrocodeine (DHC) Drugs of Abuse English Forensic GC-MS Hydrocodone Hydromorphone Morphine Opiates Oxycodone Oxymorphone Whole blood

This application note was developed for the clean-up of a non-polar solvent extract of vegetable material. The methodology is applicable to a wide range of organochlorine and organophosphorus pesticides and a number of vegetable types. The analytes are relatively non-polar, and will not be strongly retained by polar/anion exchange sorbents.

Application Notes Clordane Column Dicofol Endosulfan Food Food GC-MS Organochlorines Organophosphate Pesticide Organophosphates Pesticides Vegetables

This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL. resin, polymer, EVOLUTE,

Application Notes Biomarker Clinical Column Diethyldithiophosphate (DEDTP) Diethylphosphate (DEP) Diethylthiophosphate (DETP) Dimethyldithiophosphate (DMDTP) Dimethylphosphate (DMP) Dimethylthiophosphate (DMTP) LC-MS/MS Organophosphate Pesticide Urine

The environmental persistence of pharmaceuticals is of increasing interest, with little known regarding removal during wastewater treatment. This study investigates the potential of commercially available QuEChERS kits for the extraction and clean-up of a suite of common over-the-counter (OTC) pharmaceuticals from water and sludge cake. Extracts are analysed using LC-MS/MS.

Acetaminophen Analgesics Application Notes Cetrizine dihydrochloride Diclofenac Diphenhydramine hydrochloride English Environmental LC-MS/MS Over the counter pharmaceuticals QuEChERs Sludge cake Water

The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure. Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.

96-well plate Application Notes Clinical English LC-MS/MS Oxytocin Peptides Pharma Serum Vasopressin

The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed to cleave glucuronide moiety and produce the free form of drug. The target analytes can them be extracted from the urine matrix using EVOLUTE CX solid phase extraction cartridges. LOQs range from 5 ng/mL to 0.5 ng/mL with recoveries above 80% and RSD <10% Pain Management, EVOLUTE, CX, SPE, Solid Phase Extraction, Polymer, LC-MS-MS, Cocaine, Diazepam, Heroin, Morphine, Oxycodone, Fentanyl, Diazepam, Mixed Mode, RapidTrace

Alprazolam Application Notes Buprenorphine Butylone Clinical Clonazepam Codeine Column Fentanyl Flunitrazepam Hydrocodone Hydromorphone LC-MS/MS Meperidine Methadone Morphine Naloxone Naltrexone Oxycodone Oxymorphone RapidTrace Temazepam Urine

This application note describes a solid phase extraction (SPE) protocol for the extraction of patulin from clear apple juice using ISOLUTE Myco SPE columns, for analysis by LC-MS/MS. This method achieves high analyte recoveries with RSDs below 10%. mycotoxin, patulin, apple juice, LCMSMS

Apple juice (clear) Application Notes Column Food LC-MS/MS Mycotoxin Patulin