Developed in collaboration with the OC Crime Lab, this poster describes the extraction of 25 benzodiazepines and sedatives from various matrices using ISOLUTE SLE+ Supported Liquid Extraction columns. This poster was presented at SOFT 2013.

2-Hydroxyethylflurazepam 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Aqueous Benzodiazepines Brain Bromazepam Chlordiazepoxide Clonazepam Column Demoxepam Desalkylflurazepam Diazepam English Estazolam Flunitrazepam Flurazepam Forensic Hydroxyalprazolam Hydroxytriazolam LC-MS/MS Liver Lorazepam Midazolam Nitrazepam Nordiazepam Oxazepam Phenazepam Pig's Blood Posters Stomach contents Temazepam Triazolam Urine Zaleplone Zolpidem

User report: Initiator. Prof. Nakamura at the Gakushuin University has described the Initiator as an essential tool for his group’s cutting-edge research. We asked Prof. Nakamura for his views on the latest trends in the fields of organic synthesis and drug discovery.

Customer Cases

This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012. EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,

ACTH (1-24) Alpha-Endorphin Angiotensin Fragment 1-7 Angiotensin II, Elafin Helicobacter pylori (2-20) LC-MS/MS Myelin Glycoprotein fragment (35-55) rat Peptides Pharma Plasma Posters Vasoactive Intestinal Fragment 1-12

This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices. Pittcon 2014

96-well plate ACTH (1-24) Angiotensin Fragment 1-7 Angiotensin II, Clinical Elafin Endorphin English Glycoprotein myelin Helicobacter pylori (2-20) LC-MS/MS Peptides Pharma Plasma Posters Serum Vasoactive Intestinal Fragment 1-12

The rapid synthesis of the non-steroidal anti-inflammatory agent Fenclofenac (2-(2,4-dichlorophenoxy)phenyl acetic acid) under microwave irradiation is reported. Modified reaction conditions of traditional Ullmann ether coupling resulted in high yields of the diaryl ether. The Willgerodt–Kindler transformation and subsequent hydrolysis of thioamide resulted in 52% overall yield of Fenclofenac.

Application Notes

This application note describes the fast extraction and quantitation of 10 different antidepressant drugs in urine using ISOLUTE SLE+ 1 mL columns.

Amitriptyline Clinical Clomipramine Column Desipramine Doxepin English Forensic Imipramine Nortryptiline Paroxetine Sertraline Tricyclic Antidepressant Trimipramine Urine

The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step. A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.

6-acetyl codeine 6-acetylmorphine (MAM) 96-well plate Alprazolam Application Notes Basic Buprenorphine Clinical Clonazepam Codeine Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydromorphone LC-MS/MS Morphine Naltrexone Nitrazepam Normeperidine Oxazepam Oxycodone Oxymorphone Pain Management Temazepam Urine

This application note describes a method of extraction for metanephrine and normetanephrine from pooled plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates. A new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating traditional steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach. This method was optimized for pooled plasma.

96-well plate Application Notes Catecholamines Clinical English LC-MS/MS Metanephrine Normetanephrine Plasma

This application note describes a method of extraction for metanephrine and normetanephrine from synthetic urine using EVOLUTE® EXPRESS WCX 96-well SPE plates. A new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach. This method was optimized for synthetic urine.

96-well plate Application Notes Catecholamines Clinical English LC-MS/MS Metanephrine Normetanephrine Synthetic urine

This application note describes a method for sample preparation of different matrixes prior to amino acid analysis according to IS ISO 13903:2005 or the similar AOAC Official Method 994.12 using Biotage® Initiator+ to speed up the process.

Application Notes

Sanford Burnham Prebys Case Study. Fewer transfers and the ability to evaporate difficult solvents right in the scintillation vial has made Biotage® V-10 the go-to evaporator at Sanford Burnham Prebys (SBP), a top medical research institute.

Customer Cases

Biotage FLASH 400™ Production-Scale System The FLASH 400 is a complete skid-mounted system designed for large-scale flash chromatography and adsorption purification. The FLASH 400 uses prepacked cartridges and radial compression for performance and reliability. Built with industrial-grade components that are appropriate for operations under FDA regulations and cGMP standards, the FLASH 400 is rapidly becoming the first choice of pharmaceutical and contract manufacturing companies around the world for critical purification applications.

Product Notes

Manuals & User Guides

English Product Notes

Flash chromatography is the primary technology for separating, purifying, and isolating both synthetic organic compounds and natural products. Until recently, automated flash purification systems were limited to user-selected detection and collection wavelengths to fractionate separated compounds from sample mixtures. In this application note we demonstrate how the advances in Isolera Spektra are used in the purification of a spinach extract.

Application Notes

Isolera Spektra provides definitive fraction purity assessment in both 2-D and 3-D prior to any post flash analysis. With this information chemists quickly know if a fraction contains a pure product suitable for further mass and structure confirmation or whether the impure fractions must be re-purified. This knowledge improves synthesis throughput and avoids any embarrassment of submitting impure fractions for analytical evaluation.

Application Notes English

Improved Separation Performance Using Biotage®SNAP Bio Wide Pore media Cartridges – Part 1

Application Notes

Improved separation Performance with Biotage® SNAP Bio wide pore Media cartridges

Application Notes

The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. Luckily, there is technology that solves this problem.

Application Notes

Flash purification is a separation technique developed in 1978 by Professor W.C. Still that uses a stationary phase (a column or cartridge filled with an insoluble solid support) and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.

Brochures