Melamine is traditionally used in making plastics, however, it’s low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows recoveries of 88-90% with RSDs <10%. Melamine, food scare, china, EVOLUTE, cation exchange, RapidTrace, Automation, SPE, sample prep,

Application Notes Column Food Food contaminant LC-MS/MS Melamine Milk RapidTrace

This method demonstrates the extraction of Mephedrone (MCAT) from a range of biological fluids including plasma, urine and whole blood. This extraction is achieved through cation exchange using EVOLUTE CX, a mixed-mode resin-based SPE sorbent. Analytes were quantified using LC-MS/MS and recoveries were all above 90%. SPE, ION EXCHANGE, EVOLUTE, CX, STRATA X, OASIS, DOA

Application Notes Biological fluids Forensic LC-MS/MS Mephedrone Plasma Urine Whole blood

The screening for elevated levels of Methylmalonic Acid (MMA) in serum is commonly used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in mammals. The significance of this screening is important for the detection of inborn metabolic disorders that can lead to the build up MMA in human biological systems

96-well plate Acidic Biomarker Column English LC-MS/MS Methylmalonic acid (MMA) Posters Serum

This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS AX solid phase extraction plates. Samples are prepared using the EVOLUTE EXPRESS load-wash-elute procedure. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a simple ISOLUTE® PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts. ISOLUTE PPT+ Protein Precipitation plates offer a fast and extremely simple sample preparation approach. Serum proteins are efficiently removed from the sample using an in-plate, ‘solvent first’, protein crash and filter procedure, which is easily automated using the Biotage Extrahera system. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes the extraction of methylmalonic acid from serum, prior to LC-MS/MS analysis. An effective and efficient ISOLUTE® SLE+ protocol optimized for the 200 μL capacity 96-well plate format is used. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs below 10%. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a supported liquid extraction protocol for the extraction of methylisothiazolinone from sun lotion, prior to GC/MS analysis.

Antimicrobial Application Notes Clinical Column English GC-MS MI (methylisothiazolinone) Methylisothiazolinone Preservative Sun Lotion Sunscreen

This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of a range of mycotoxins from animal feed using ISOLUTE® Myco SPE columns with LC-MS/MS analysis.

Aflatoxin B1 Animal feed Application Notes Column Deoxynivalenol (DON) English Food Fumonisin B1 HT-2 mycotoxin LC-MS/MS Mycotoxin Ochratoxin A T-2 mycotoxin Zearalenone

This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of a range of mycotoxins from cereal based infant food using ISOLUTE® Myco with UHPLC-MS/MS. The method achieves high recoveries of relevant mycotoxins from a cereal based infantfood with low %RSDs and LOQs.

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Column English Food Fumonisin B1 HT-2 mycotoxin Infant food (cereal) LC-MS/MS Mycotoxin Ochratoxin A T-2 mycotoxin Zearalenone

This application note describes a solid phase extraction (SPE) protocol for the extraction of a range of mycotoxins from grain (wheat, barley, maize, wheat flour) using ISOLUTE Myco SPE columns for LC-MS/MS analysis. The method described achieves high recoveries of relevant mycotoxin classes from a range of different grain matrices with RSDs and LOQs that meet the requirements of EU regulations. mycotoxins, grains, LC-MS/MS, food safety

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Barley Column Ergocornine Ergocryptine Food Fumonisin B1 Grain HT-2 mycotoxin LC-MS/MS Maize Mycotoxin Ochratoxin A T-2 mycotoxin Wheat Wheat flour Zearalenone

This application note describes the extraction of mycotoxins (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A) from nuts (brazil nut, groundnut (peanut) using ISOLUTE Myco SPE columns with LC-MS/MS analysis

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Bovine kidney Clinical Column Food Groundnut (Peanut) LC-MS/MS Mycotoxin Nuts Ochratoxin A

This application note describes the extraction of the immunosuppressant drug mycophenolic acid and its metabolite mycophenolic acid glucuronide from fortified serum using ISOLUTE SLE+ in a 96-well plate format.

96-well plate Acidic Application Notes Clinical English Immunosuppressant LC-MS/MS Mycophenolic Acid Mycophenolic Acid Glucuronide Serum

Immunosuppressant drugs are instrumental in preventing organ and tissue rejection in patients undergoing transplant surgery. Mycophenolic Acid is a common immunosuppressant drug used in patient transplant therapy. The ability to monitor the trough levels in patients to evaluate dosing is important for the administering of the drug. The free drug and its glucuronidated metabolite can be found in patient serum. The ability to extract the free drug and metabolite in patient serum is supported by using a fast and efficient sample preparation extraction method called Supported Liquid Extraction (ISOLUTE SLE+). The free drug and metabolite can be recovered from serum using ISOLUTE SLE+ with high enough efficiency to allow for quantitation at target trough levels. MSACL 2014

96-well plate Clinical English Immunosuppressant LC-MS/MS Mycophenolic Acid Mycophenolic Acid Glucuronide Posters Serum

This poster describes the extraction of the commonly used immunosuppressant drug mycophenolic acid and its glucuronide metabolite from serum using ISOLUTE SLE+ supported liquid extraction plates. ASMS 2014

96-well plate Clinical Immunosuppressant LC-MS/MS Mycophenolic Acid Mycophenolic Acid Glucuronide Posters Serum

This application note describes the extraction of a suite of NBOMe designer drugs from oral fluid matrix with analysis by laser diode thermal desorption (LDTD)-MS/MS.

25B-NBOMe 25C-NBOMe 25D-NBOMe 25E-NBOMe 25H-NBOMe 25N-NBOMe 25T2-NBOMe Application Notes Column Drugs of Abuse English Forensic LDTD-MS/MS Legal highs NBOMes Oral fluid Saliva

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of nicotine, cotinine, 3-OH-cotinine, nornicotine, norcotinine and anabasine from urine, serum, plasma and whole blood using ISOLUTE® SLE+ plates with LC-MS/MS detection. nicotine cotinine nornicotine norcotinine 3-OH cotinine anabasine smoker

3-OH-cotinine Anabasine Application Notes Clinical Cotinine LC-MS/MS Nicotine Nicotine metabolites Norcotinine Nornicotine Plasma Serum Urine Whole blood

Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase.

Application Notes Column DMZOH Dimetridazole (DMZ) Food IPZOH Ipronidazole (IPZ) LC-MS/MS MNZOH Metronidazole (MNZ) Milk Nitroimidazoles Ronidazole Veterinary drugs Water

This Application Note highlights the use of supported-liquid Extraction to isolate three common NSAIDs, Sulindac, Flurbiprofen and Ibuprofen, from human plasma. Biotage, Ostro, Liquid liquid extraction, supported liquid extraction, Extrelute

Application Notes Clinical Flurbiprofen Ibuprofen LC-MS/MS NSAIDs Plasma Sulindac