Part No: 02140-AIssued year: 2014File size: 3.76mbFile type: pdf
INTRODUCING BIOTAGE® FLASH 150I, COMPONENTS OF BIOTAGE® FLASH 150I, INSTALLATION/GETTING STARTED, HOW TO USE BIOTAGE® FLASH 150I, TROUBLESHOOTING, PRICE LIST AND ORDERING INFORMATION, OPTIMIZING FLASH SEPARATIONS, FLASH CHROMATOGRAPHY WORKBOOK, and CE INFORMATON ADDENDUM.
Part No: 355977-FIssued year: 2014File size: 1.32mbFile type: pdf
Translated versions of the Safety chapter available in the Biotage® Initiator Installation and Safety document (P/N 355976). Languages: Danish, Dutch, Finnish, French, German, Italian, Japanese, Spanish, and Swedish.
Part No: 355977-GIssued year: 2015File size: 2.37mbFile type: pdf
Translated versions of the Safety chapter available in the Biotage® Initiator+ Installation and Safety document (P/N 355976). Languages: Danish, Dutch, Finnish, French, German, Italian, Japanese, Spanish, and Swedish.
Microwave irradiation is still the most effective solution for providing a fast, precise and efficient heating method for synthesizing peptides, peptoids, PNA and peptidomimetics with higher purity and yield compared to conventional synthesis methods. The Biotage® Initiator+ Alstra is a fully automated microwave peptide synthesizer with built in flexibility for both small and large scale synthesis.
Part No: PPS390.V.1Issued year: 2016File size: 1.05mbFile type: pdf
Biotage® MP-Borohydride is a macroporous, solid-supported borohydride that is a solid-supported equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent used for the reduction of carbonyl
compounds and imines, and the reductive amination of aldehydes and ketones.
Part No: PPS392.V.2Issued year: 2016File size: 1.1mbFile type: pdf
Biotage® MP-Cyanoborohydride is a macroporous polymer-supported cyanoborohydride which is a solid-supported equivalent of tetraalkylammonium cyanoborohydride. The bound cyanoborohydride can be utilized as a versatile reducing agent for the reductive amination of carbonyl compounds and reduction of imines.
Part No: PPS391.V.1Issued year: 2016File size: 1.79mbFile type: pdf
Biotage® MP-Triacetoxyborohydryde is a macroporous polystyrene-supported equivalent of tetraalkylammonium triacetoxyborohydride. MP-Triacetoxyborohydride has primary applications in the reductive amination of aldehydes and ketones under neutral or mildly acidic reaction conditions.
Part No: PPS404.V.1Issued year: 2016File size: 1.41mbFile type: pdf
PS-Benzaldehyde is the resin-bound equivalent of benzaldehyde. This resin is useful for scavenging various nucleophiles, including primary amines (selectively compared with secondary amines), hydrazines and also carbon-based nucleophiles such as Meldrum’s acid and organometallics.
Part No: PPS402.V.1Issued year: 2016File size: 0.95mbFile type: pdf
PS-Carbodiimide is a neutral, bound carbodiimide that can be used for the synthesis of amides and esters. PS-Carbodiimide may also be used for the synthesis of pentafluorophenyl (PFP) activated esters (Table 3) and N-hydroxysuccinimidyl esters
Part No: PPS393.V.1Issued year: 2016File size: 0.98mbFile type: pdf
Biotage® PS-HOBt(HL) is a sulfonamide-linked, polymersupported
equivalent of 1-hydroxybenzotriazole (HOBt). PS-HOBt(HL) is used to generate bound HOBt active esters, which can either be made and used in situ, or isolated and stored as stable intermediates.
Part No: PPS400.V.1Issued year: 2016File size: 1.3mbFile type: pdf
Biotage®PS-Isocyanate is a 1% cross-linked poly(styreneco-divinylbenzene) which has pendent benzylisocyanate functionality. The resin can readily scavenge excess nucleophiles, which are often used to drive reactions to completion.
Part No: PPS401.V.1Issued year: 2016File size: 2.11mbFile type: pdf
PS-PPh3-Pd resin is a polystyrene-supported equivalent of the small molecule catalyst tetrakis(triphenylphosphine) palladium(0) [Pd(Ph3P)4]. The primary application for the resin is as a catalyst for Suzuki-Miyaura coupling reactions between arylboronic acids and aryl halides.
Part No: PPS382.V.1Issued year: 2016File size: 1.63mbFile type: pdf
Biotage® PS-TBD is a polymer-supported base, which applications are Alkylation of phenols and amines; esterification of carboxylic acids using alkyl halides; alkylation of activated methylene compounds; dehalogenation of organic halides; high throughput synthesis of aryl triflates and aryl nonaflates; Williamson ether synthesis
Part No: PPS389.V.1Issued year: 2016File size: 2.64mbFile type: pdf
Biotage® PS-Triphenylphosphine is a diphenylphosphinated polystyrene resin that is a solid-supported equivalent of triphenylphosphine. The resin can readily convert alcohols or carboxylic acids to the corresponding chlorides or acid chlorides in carbon tetrachloride.
Part No: PPS437Issued year: 2016File size: 0.19mbFile type: pdf
Biotage® SNAP Bio flash cartridges were developed with a small
particle size (20 μm) and large pore size (300 Å) to provide
increased resolution and effective separation of complex
peptide mixtures. Depending on the quality of the peptides
synthesized, flash chromatography can be used either as the
sole method of purification or as a front end clean-up prior to
SNAP Bio offers close to prep-HPLC performance in a flash
format at a fraction of the cost
Part No: TN-0016.0108Issued year: 2008File size: 0.4mbFile type: pdf
Biotage SNAP cartridges can be used on any flash system.
The easy-to-use Luer-lok® connections do not need a
compression module to operate at 100 psi. Made from USP
Class VI materials, Biotage SNAP cartridges offer 7 types of
loading techniques including 3 internal dry loading options.
Packed to provide the highest plate count of any 50μm particle
cartridge on the market, SNAP provides increased loading
capacity and better compound separations.
Part No: TN-0025.0508Issued year: 2008File size: 0.35mbFile type: pdf
SNAP KP-NH cartridges contain amine functionalized silica.
This optimized media shields synthetic organic amines from
acidic silanols providing improved selectivity, peak shape,
purity and yield (Figure 1). Unlike traditional silica and 1°
amine (propyl amine) bonded silica, KP-NH doesn’t require the
use of chlorinated solvents or amine additives.
Part No: Issued year: 2009File size: 0.06mbFile type: pdf
SNAP flash cartridges provide several sample load options including both internal and external liquid and dry loading. This
Quick-start Guide provides sample load guidelines and loading technique suggestions, which should help you get the most
from your SNAP cartridges.
Part No: Issued year: 2008File size: 0.09mbFile type: pdf
SNAP flash cartridges provide several sample load options including both internal and
external liquid and dry loading. This Quick Start Guide provides sample load guidelines and
loading technique suggestions, which should help you get the most from your SNAP
Part No: TN-SNAP-RPC-0211Issued year: 2011File size: 1.11mbFile type: pdf
Reversed-phase flash chromatography is a very popular
purification technique using a non-polar stationary phase.
Main application areas include separation of polar, ionizable
and highly lipophilic compounds which cannot easily be
separated by normal-phase techniques.
Part No: TN-0032.0112Issued year: 2012File size: 0.75mbFile type: pdf
Precision engineered Biotage SNAP Ultra cartridges deliver double
the purification capacity utilizing small particle spherical silica with
an 40% increase in surface area. This proprietary silica reduces
peak width and provides higher concentration fractions with less
Part No: PPS425Issued year: 2016File size: 2.58mbFile type: pdf
Biotage® V-10 Touch rapidly dries samples dissolved in both aqueous and organic solvents. Combined with the Gilson liquid handling robot automatically transfers your purified samples to V-10 Touch for sequential automated evaporation. This brochure lists all evaporation vials, adapters and accessories.
Part No: UI303Issued year: 2013File size: 4.69mbFile type: pdf
The Biotage® VacMaster™ is a state-of-the-art sample processing station. It incorporates innovative design features that make simultaneously processing multiple samples easier than ever before. For scientists working with biological fluids, these features and operating procedures provide a safe system.
Part No: UI327Issued year: 2015File size: 1.4mbFile type: pdf
Biotage® ACT Plate Adapter (patent pending) is designed to reduce or eliminate the occurrence of well-to-well cross contamination (cross talk) during extract evaporation, and fits on top of the 96-well collection plate during evaporation.
Part No: P137Issued year: 2015File size: 0.37mbFile type: pdf
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
Part No: P137v2Issued year: 2016File size: 0.39mbFile type: pdf
This poster investigates various parts of the SPE method development process to develop a highly sensitive LC-MS/MS method for analysis of catecholamines (epinephrine, norepinephrine, dopamine).
A number of steps are optimized to improve sensitivity of the
analytes. This includes reducing the buffer strength in the mobile
phase, reducing the buffer strength of washes, careful pH control
throughout the extraction, avoiding an evaporation step and
incorporating IPA in the reconstitution solvent.
Part No: Issued year: 2012File size: 0.11mbFile type: pdf
The following organization’s Quality Management System has been assessed and registered by Intertek Certification AB as conforming to the requirements of: SS-EN ISO 9001:2008
The Quality Management System is applicable to: Development and production of specialty polymers (MIPs and NIPs) for industrial separations and laboratory trace analysis.
Part No: CM-INT-0810Issued year: 2010File size: 0.25mbFile type: pdf
ChemMatrix is a patented, 100% PEG (polyethylene glycol)
based resin developed by PCAS BioMatrix that offers substantial
advantages over traditional PEG based polystyrene resins for
solid phase peptide synthesis.
Part No: RT-CM-0111Issued year: 2010File size: 0.09mbFile type: pdf
In order to keep your RapidTrace® workstation clean, in good working order and free from contamination it is of course essential to
routinely clean and purge the fluid path. Whichever matrix and reagents are running through the system, traces of these liquids will
be left after use; proteins from plasma, precipitates from buffers or even particulates from oral fluid. Over time if the following
cleaning schedule is not observed these contaminants could cause blockages leading to malfunctions and/or cross contamination.
Part No: P136Issued year: 2015File size: 0.6mbFile type: pdf
This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The manual extraction protocols were transferred to an SPE automation platform (Biotage® Extrahera)and method performance versus manual processing compared.
MSACL EU 2015
Part No: PPS362Issued year: 2014File size: 1.23mbFile type: pdf
This product sheet compares automated sample preparation using the Biotage®Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled
stripped plasma using a supported liquid extraction procedure.
Part No: PPS366Issued year: 2014File size: 1.76mbFile type: pdf
Automated sample preparation using the Biotage®Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Part No: PPS361Issued year: 2014File size: 1.28mbFile type: pdf
This document compares automated sample preparation using the Biotage®
Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Part No: P142Issued year: 2016File size: 0.57mbFile type: pdf
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Part No: PPS430Issued year: 2016File size: 0.97mbFile type: pdf
At the Forensic Medicine Lab at Toho University, researchers use ISOLUTE® SLE+ columns from Biotage. When dealing with samples that easily form emulsions like urine or blood, it allows researchers to use the established liquid-liquid extraction technique, saving significant amount of time on analysis. We spoke with the Head of the Forensic Medicine Lab Professor Masaru Terada.
Part No: Issued year: 2009File size: 0.12mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB and a number of acquisitions were performed within the Medicinal Chemistry sector during the years of 2003-2005. After divesting the business area Biosystems in October 2008, the Company’s business is today made up entirely of the business area Discovery Chemistry. Biotage’s headquarters is located in Uppsala.
Part No: Issued year: 2010File size: 0.2mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (”the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2011File size: 0.72mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (“the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2013File size: 0.11mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company is mainly active within analytical and organic chemistry.
Part No: PPS445Issued year: 2017File size: 0.62mbFile type: pdf
Ease of use is what stands out as the top feature of Isolera™ flash chromatography
system for Professor Anna Bernardi, head of the synthetic organic chemistry
research group at the University of Milan. Her current research goal: developing
sugar-like molecules, called glycomimetics, for healthier life.
Part No: PPS443Issued year: 2017File size: 2.31mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: Issued year: 2011File size: 0.07mbFile type: pdf
Through innovative patented technology based on molecularly imprinted polymers (MIPs)
you will experience improved clean up in your processes. With a high probability of success,
unwanted contaminants or high value desirables can be selectively extracted from your
processes, resulting in more efficient production and cleaner products.
Part No: P102Issued year: 2014File size: 0.6mbFile type: pdf
Designed polymers are a class of selective resins with engineered selectivities for particular target molecules or ‘classes’ of molecules. These designed polymers are obtained by careful tuning of their surface chemistry and morphology which allows them to exhibit unique separation capabilities. The tailored and optimized selectivity of designed polymers is utilized to conduct difficult separations that are not able to be accomplished with conventional separation resins or other techniques.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: Issued year: 2014File size: 0.12mbFile type: pdf
This poster describes the development and validation of a method for supported liquid extraction of serum cortisol, with analysis by UPLC-MS/MS. The aim of this study was to develop a candidate reference method that could then be used to underpin the UK NEQAS Cortisol scheme.
MSACL EU 2014