How to use the Atmospheric Solid Analysis Probe on Dalton 2000

Manuals & User Guides

ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction. Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,

Aliphatic hydrocarbos Application Notes Column English Environmental Polyaromatic hydrocarbons Soil

Over the past decade, the need for non-invasive drug screening that that precludes sample adulteration has become attractive. As a result, detection using oral fluid devices for Drugs of Abuse (DOA) has come to the vanguard of the scientific community. The use of Supported Liquid Extraction (ISOLUTE® SLE+) prior to LC/MS or GC/MS can improve sample cleanliness without forfeiting sample detection within a diverse panel of DOAs. Here, we demonstrate the effects of altering elution solvent polarity and pH for sample pretreatment upon the simultaneous recovery of 34 compounds comprised of opioids, benzodiazepines, and stimulants to directly measure the effects of the oral fluid buffer, OraSure™, upon extraction and signal intensity at presumed LOQs. ASMS 2017

6-acetylmorphine (MAM) 7-Aminoclonazepam Alprazolam Amphetamine Benzodiazepines Benzoylecgonine Buprenorphine Clinical Clonazepam Cocaine Cocaines Codeine Column Cotinine Diazepam EDDP English Forensic Hydrocodone Hydromorphone LC-MS/MS Lorazepam MDA MDEA MDMA Meperidine Methadone Methamphetamine Midazolam Morphine Norbuprenorphine Nordiazepam Opiates Opioid Oral fluid Oxazepam Phencyclidine Phencyclidine (PCP) Posters Stimulants Temazepam Triazolam α-OH Triazolam α-OH alprazolam

Biotage® Endeavor™ catalyst screening system was used to investigate the asymmetric hydrogenation of 4-aryl coumarins to 4-aryl-3,4-dihydrocoumarin, an important intermediate in the synthesis of endothelin receptor antagonists.

Application Notes

The Atlantis pressure scale-up workstation performs and optimizes gas/liq and gas/liq/solid, heterogeneous reactions with the unique ability to monitor live gas consumption. Process Chemists can easily load experiments with push-button hardware and precisely control and monitor reactions with simple-to-use software. The Atlantis system controls reaction parameters automatically to eliminate sample degradation and in addition, provides concise reaction data reports with live molar uptake rates for better analysis.

Product Notes

This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual (Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.

96-well plate Application Notes English Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-(5-hydroxypentyl) JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Synthetic Cannabinoids (SPICE) TECAN UR-144 UR-144-(5-hydroxypentyl) UR-144-(pentanoic acid) UR144-(5-chloropentyl) Urine XLR-11

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(ghi)perylene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

Introduction • Solid-phase peptide synthesis (SPPS) is still often faced with challenges in the assembly of long and ‘difficult’ sequences, e.g. due to aggregation and steric hindrance giving rise to incomplete reactions. These problems have only partly been solved by new coupling reagents and solid supports. • Precise microwave heating has emerged as one new parameter for SPPS, in addition to coupling reagents, resins, solvents etc.

Posters

Introduction • Solid-phase peptide synthesis (SPPS) is still often faced with challenges in the assembly of long and ‘difficult’ sequences, e.g. due to aggregation and steric hindrance giving rise to incomplete reactions. These problems have only partly been solved by new coupling reagents and solid supports. • Precise microwave heating has emerged as one new parameter for SPPS, in addition to coupling reagents, resins, solvents etc.

Posters

Cyclosporine is an immunosuppressant used in organ transplants to overcome rejection. Following the transplant of a heart, liver, kidney or lung, a patient must be regularly tested for the rest of their lives. Blood testing intervals vary from 1 month to 6 months and this rapid reference method is ideal for batch tests with the advantage of no metabolite interfer-ence which is always found in immunological methods.

Application Notes Liver

The removal of interferences and sample concentration are essential steps in trace level detection of pesticides, sheep dips, herbicides and PCBs in river water, wastewaters and trade effluents. The following methods were developed by the Environment Agency National Laboratory Service facility at Nottingham using the Biotage RapidTrace for automated solid phase extraction and the TurboVap II for evaporation. The procedures are UKAS accredited and can reach detection limits of 0.001 ug/Litre for some compounds.

Application Notes Environmental Environmental contaminants Organophosphates RapidTrace Water

EPA methods 8260 & 8270 cover the analysis of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in ground & surface waters. This also now includes 1,4-dioxane and PCBs. This application note will focus on the automated SPE of the sample rather than the detection which usually involves GCMS or LCMS. NB The columns and reagents used for soils and sediment analysis differ to ground water extraction.

Application Notes Environmental Environmental contaminants RapidTrace Soil

The extraction and segregation of Organic compounds from core samples taken from marine sediments is used to predict climate conditions, reconstruct sea temperatures and identify the type of vegetation from the C3 ( Temperate cli-mate) and C4 ( Tropical Climate) isotopic mass spectroscopy ratio analysis.

Application Notes

With the widespread clinical use of antidepressant drugs comes the need to accurately measure therapeutic lev-els to determine proper dosage, as well as check for toxic levels and patient compliance. More and more laboratories are shifting their therapeutic drug monitor-ing from liquid extraction to solid phase extraction. A large clinical laboratory has automated the process of extracting these drugs from serum using the RapidTrace SPE Workstation.

Application Notes

When analyzing human urine for drugs of abuse, one of the most common tests is for the cocaine metabolite Benzoylecgonine (BZE). A contract laboratory has automated this labor intensive procedure using the RapidTrace.

Application Notes Benzoylecgonine Column Drugs of Abuse Forensic GC-MS RapidTrace Urine

Using the TLC to step gradient is simple and fast requiring only a minute or so to enter the data into the Isolera and create the method. The methods are scalable to larger cartridges increasing purification savings even more.

Posters

Synthesis of branched peptides is very challenging. Here we show the synthesis of a complex multi-branched peptide and how this process can be simplified using Branches™.

Application Notes