How to use the Atmospheric Solid Analysis Probe on Dalton 2000

Manuals & User Guides

ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction. Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,

Aliphatic hydrocarbos Application Notes Column English Environmental Polyaromatic hydrocarbons Soil

Over the past decade, the need for non-invasive drug screening that that precludes sample adulteration has become attractive. As a result, detection using oral fluid devices for Drugs of Abuse (DOA) has come to the vanguard of the scientific community. The use of Supported Liquid Extraction (ISOLUTE® SLE+) prior to LC/MS or GC/MS can improve sample cleanliness without forfeiting sample detection within a diverse panel of DOAs. Here, we demonstrate the effects of altering elution solvent polarity and pH for sample pretreatment upon the simultaneous recovery of 34 compounds comprised of opioids, benzodiazepines, and stimulants to directly measure the effects of the oral fluid buffer, OraSure™, upon extraction and signal intensity at presumed LOQs. ASMS 2017

6-acetylmorphine (MAM) 7-Aminoclonazepam Alprazolam Amphetamine Benzodiazepines Benzoylecgonine Buprenorphine Clinical Clonazepam Cocaine Cocaines Codeine Column Cotinine Diazepam EDDP English Forensic Hydrocodone Hydromorphone LC-MS/MS Lorazepam MDA MDEA MDMA Meperidine Methadone Methamphetamine Midazolam Morphine Norbuprenorphine Nordiazepam Opiates Opioid Oral fluid Oxazepam Phencyclidine Phencyclidine (PCP) Posters Stimulants Temazepam Triazolam α-OH Triazolam α-OH alprazolam

Biotage® Endeavor™ catalyst screening system was used to investigate the asymmetric hydrogenation of 4-aryl coumarins to 4-aryl-3,4-dihydrocoumarin, an important intermediate in the synthesis of endothelin receptor antagonists.

Application Notes

The Atlantis pressure scale-up workstation performs and optimizes gas/liq and gas/liq/solid, heterogeneous reactions with the unique ability to monitor live gas consumption. Process Chemists can easily load experiments with push-button hardware and precisely control and monitor reactions with simple-to-use software. The Atlantis system controls reaction parameters automatically to eliminate sample degradation and in addition, provides concise reaction data reports with live molar uptake rates for better analysis.

Product Notes

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns. The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) Application Notes Column English Extrahera Forensic GC-MS Hydrolysed urine THC metabolites THC-COOH

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with ISOLUTE SLE+ columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage Extrahera, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) Application Notes Column English Forensic GC-MS Hydrolysed urine THC metabolites THC-COOH

This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual (Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.

96-well plate Application Notes English Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-(5-hydroxypentyl) JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Synthetic Cannabinoids (SPICE) TECAN UR-144 UR-144-(5-hydroxypentyl) UR-144-(pentanoic acid) UR144-(5-chloropentyl) Urine XLR-11

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

Introduction • Solid-phase peptide synthesis (SPPS) is still often faced with challenges in the assembly of long and ‘difficult’ sequences, e.g. due to aggregation and steric hindrance giving rise to incomplete reactions. These problems have only partly been solved by new coupling reagents and solid supports. • Precise microwave heating has emerged as one new parameter for SPPS, in addition to coupling reagents, resins, solvents etc.

Posters

Introduction • Solid-phase peptide synthesis (SPPS) is still often faced with challenges in the assembly of long and ‘difficult’ sequences, e.g. due to aggregation and steric hindrance giving rise to incomplete reactions. These problems have only partly been solved by new coupling reagents and solid supports. • Precise microwave heating has emerged as one new parameter for SPPS, in addition to coupling reagents, resins, solvents etc.

Posters

This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.

4790 Anthracene Application Notes Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Disk EN16691 Analytes English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Surface water