Part No: AN067Issued year: 2012File size: 1.01mbFile type: pdf
La cromatografía flash en fase normal y fase reversa facilita el aislamiento de compuestos
polares presentes en extractos de productos naturales. La combinación de avanzadas funciones
de detección tales como barrido espectral, y evaporativo de luz dispersa (ELSD) incrementa la
efectividad de la cromatografía flash en procesos de purificación de productos naturales.
Part No: P169Issued year: 2017File size: 0.23mbFile type: pdf
Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Part No: AN886Issued year: 2017File size: 1mbFile type: pdf
This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.