This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum
This application note was developed for the clean-up of a non-polar solvent extract of vegetable material. The methodology is applicable to a wide range of organochlorine and organophosphorus pesticides and a number of vegetable types. The analytes are relatively non-polar, and will not be strongly retained by polar/anion exchange sorbents.
Tags: Application Notes, Clordane, Column, Dicofol, Endosulfan, Food, Food, GC-MS, Organochlorines, Organophosphate Pesticide, Organophosphates, Pesticides, Vegetables
The following method describes the extraction and separation of non-conjugated parent, glucuronide conjugates and sulfate conjugates of a proprietary pharmaceutical from urine and bile using non-polar and anion exchange mechanisms.
Tags: Application Notes
Recomendations for SPe method development. The proposed steps are based on experience with similar analytes and matarices, but have not been verified in Biotage laboratories.
Tags: Animal feed, Application Notes
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Tags: 96-well plate, Acidic, Clinical, English, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Posters, Serum
The ISOLUTE® family of anion exchange sorbents are used to extract organic anions (acid compounds capable of exhibiting a negative charge) from both aqueous and non-aqueous matrixes. This technical
note describes their use for aqueous matrices only. Although extraction using these sorbents is by the same mechanism, each has properties which influence the way they are used.
Tags: English, Product Notes
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all belowfor download. P177: Comparison of Sample Preparation Options for the Extra...11 January 2018
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...17 July 2017
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...28 June 2017
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...15 June 2017
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...20 March 2017
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...20 February 2017
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...02 February 2016
Five new application notes from Biotage labs provide a suite of methods for effective extraction of methylmalonic acid from serum using modern sample preparation techniques and materials from ...05 January 2016
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...11 August 2015
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...08 January 2015
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|500-0002-A||ISOLUTE® SAX 25 mg/1 mL||Consumables||100||Log in for price|
|500-0005-A||ISOLUTE® SAX 50 mg/1 mL||Consumables||100||Log in for price|
|500-0005-AA||ISOLUTE® SAX 50 mg/1 mL (ASPEC Adaptor)||Consumables||100||Log in for price|
|500-0010-A||ISOLUTE® SAX 100 mg/1 mL||Consumables||100||Log in for price|
|500-0010-AA||ISOLUTE® SAX 100 mg/1 mL (ASPEC Adaptor)||Consumables||100||Log in for price|
|500-0010-B||ISOLUTE® SAX 100 mg/3 mL||Consumables||50||Log in for price|
||500-0010-C||ISOLUTE® SAX 100 mg/6 mL||Consumables||30||Log in for price|
||500-0020-B||ISOLUTE® SAX 200 mg/3 mL||Consumables||50||Log in for price|
||500-0025-P01||ISOLUTE®-96 SAX 25 mg Fixed Well Plate||Consumables||1||Log in for price|
|500-0050-B||ISOLUTE® SAX 500 mg/3 mL||Consumables||50||Log in for price|
|500-0050-BA||ISOLUTE® SAX 500 mg/3 mL (ASPEC Adaptor)||Consumables||50||Log in for price|
|500-0050-C||ISOLUTE® SAX 500 mg/6 mL||Consumables||30||Log in for price|
||500-0050-H||ISOLUTE® SAX 500 mg/10 mL (3 mL XL)||Consumables||50||Log in for price|
||500-0050-P01||ISOLUTE®-96 SAX 50 mg Fixed Well Plate||Consumables||1||Log in for price|
||500-0100-B||ISOLUTE® SAX 1 g/3 mL||Consumables||50||Log in for price|
|500-0100-C||ISOLUTE® SAX 1 g/6 mL||Consumables||30||Log in for price|
||500-0100-P01||ISOLUTE®-96 SAX 100 mg Fixed Well Plate||Consumables||1||Log in for price|
||500-0200-D||ISOLUTE® SAX 2 g/15 mL||Consumables||20||Log in for price|