Documents | A No-Drydown SPE Method for Biomarkers of Alcohol Consumption in Human Urine Using ISOLUTE® NH2 SPE Columns Prior to LC-MS/MS

A No-Drydown SPE Method for Biomarkers of Alcohol Consumption in Human Urine Using ISOLUTE® NH2 SPE Columns Prior to LC-MS/MS

This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.

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Biotage is the Global Go-To Separations Company, supporting customers from drug discovery and development through to diagnostics and analytical testing with intelligent and sustainable workflow solutions. Our expertise and top-tier separation solutions play a key role in streamlining our customers’ workflows and improving their outcomes. Headquartered in Sweden, Biotage operates globally with 700 employees, serving over 80 countries. Our company is listed on NASDAQ Stockholm (BIOT).

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A No-Drydown SPE Method for Biomarkers of Alcohol Consumption in Human Urine Using ISOLUTE® NH2 SPE Columns Prior to LC-MS/MS