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    Documents | Automated Mass Spectrometry Sample Prep, Sample Desalting by Reversed Phase PhyTip® Columns

    Automated Mass Spectrometry Sample Prep, Sample Desalting by Reversed Phase PhyTip® Columns

    Biotage has an automated process to desalt or “clean up” proteins/peptides using C18 PhyTip® columns in order to streamline sample preparation for mass spectrometry analysis up to 96 samples at a time. When preforming a reverse phase purification using a manual pipette, back pressure while aspirating solutions can inconvenience the operator and cause inconsistent results. PhyTip columns perform the clean-up with the consistency, ease, and scalability that go hand in hand with automation. With the high capacity and versatility in protein binding, the C18 PhyTip column is the optimal choice in developing a fluid MALDI or LC-MS workflow.

    » Bind a wide range of protein/peptide sizes
    » High absolute binding capacity
    » High recovery, low peptide loss
    » Compatible with common mass spectrometry solvents
    » Compatible with major automation liquid handling robots

    C18 Tip   Capacity (μg*) Recovery  Range
    PhyTip® Column 19.3 ~85%   <50 kDA
    ZipTip® 8.33 ~85%  <50 kDA
    OMIX® Tip  8.00 ~85%  No Data

    *Capacity is measured in μg of protein per μL of silica.

     

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