Extraction of a Range of Immunosuppressants From Whole Blood Using ISOLUTE SLE+ for LCMS/MS Analysis

Immunosuppressants inhibit or prevent the activity of the immune system used to prevent rejection of transplanted organs and treat autoimmune diseases. Due to the risks of immunodeficiency, those under treatment must undergo constant therapeutic drug monitoring (TDM) requiring reliable and robust analytical techniques for quantification of these drugs. Current methodologies use immunoassay techniques to measure immunosuppressant levels in patients which are expensive, time consuming and susceptible to issues with cross reactivity. The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%. Immunosuppressant, Tacrolimus, Sacrolimus, Everolimus, Cyclosporin A, Clinical, SLE+, SLE, Supported Liquid Extraction, immune, Clinical, Whole Blood, immunoassay,