Extraction of Multiple Mycotoxins From Cereal Based Infant Food Using ISOLUTE® Myco prior to UHPLC MS/MS Analysis

Introduction

This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of a range of mycotoxins from cereal based infant food using ISOLUTE® Myco with UHPLC-MS/MS. Mycotoxins are toxic metabolites produced by fungal molds on food crops. Regulation and legislation for testing of mycotoxin contamination has established which mycotoxins are prevalent on a wide variety of food crops. This application note describes an SPE protocol appropriate for LC-MS/MS analysis of a range of mycotoxins found on cereal based infant food.

The method described in this application note achieves high recoveries of relevant mycotoxins from a cereal based infant food with low %RSDs and LOQs. ISOLUTE Myco solid phase extraction columns provide robust, reliable sample preparation for multiple mycotoxin classes from a wide range of foodstuffs. Using a single, easy to use sample preparation product, along with optimized matrix specific application notes, scientists can prepare diverse food/crop samples for analysis by LC-MS/MS.

Analytes

Aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, fumonisin B1, zearalenone, HT-2, T-2 mycotoxin.

Sample preparation procedure

Format: ISOLUTE® Myco 60 mg/3 mL (Tabless), part number 150-0006-BG
Sample pre-treatment:
1. Sample processing: Grind the sample (50 g, Cow&Gate®  Sunny Start Baby Wheat Flakes were used in this application note) with a burr-grinder or equivalent device. Store the ground sample in a sealed container at room temperature until required.

2. Extraction: Mix the ground sample (5 g) with NaCl (1g) and 60% methanol (aq) (25 mL) and place on a shaking table for 30 minutes. Transfer the extract to a 50 mL centrifuge tube and centrifuge at 3000 g for 10 minutes.
3. Dilution: Take the supernatant (7 mL), transfer to a new 50 mL centrifuge tube and dilute with water (35 mL). Centrifuge diluted extract at 3000 g for a further 10 minutes.