Part No: PPS385Issued year: 2015File size: 4.87mbFile type: pdf
Our carefully selected portfolio of industrial scale products have a proven track record of successful applications and use in scale-up projects. We can support the discovery, development and manufacturing of customer pharmaceutical and biotechnology products, from pre-clinical, phase I, II and III to small scale commercial operations.
Part No: TN-0028.1222Issued year: 2012File size: 1.6mbFile type: pdf
The Initiator+ is a flexible system that utilizes all Biotage vials, from 0.2 to 20 mL,
in any order or combination, at any time without system modifications, delivering greater flexibility and direct scale-up from milligrams to grams.
Part No: TN-0029.0611Issued year: 2011File size: 1.07mbFile type: pdf
In peptide synthesis mode, this is the perfect tool for chemists synthesizing peptides and peptidomimetics, including difficult modifications and labeling of sequences, by using single or multi-step procedures. The intuitive Initiator 4.0 software controls the instrument functions and by using the pre-defined methods, synthesis can be started immediately.
Part No: AN090Issued year: 2014File size: 1.95mbFile type: pdf
In mass detection, the presence of ions can suppress peaks
from the analytes of interest. This study concludes that
Isolera™ Dalton performs successful separations even with
ions present in the sample.
Part No: AN112-HORIssued year: 2016File size: 1.23mbFile type: pdf
Dioxins are of great environmental concern due to their teratogenic, mutagenic, and carcinogenic impact. These lipophilic compounds bioaccumulate in humans and wildlife and can have half lives of up to 132 years, making them persistent organic pollutants (POPs). Traditional SPE requires a filtration step to remove particulates when using SPE cartridges, because of clogging issues.
Part No: P181Issued year: 2018File size: 0.19mbFile type: pdf
Peptide purification using standard reversed phase HPLC methods are hampered by low loading capacity, resulting in purifications that demand significant time investment. Recently, the use of reversed phase flash chromatography has increased in popularity for peptide purification due to the significant reduction of purification time,
enabled by the increased loading levels of the larger stationary phase particles. Resolution, though, is somewhat diminished with the larger particle size, demanding creative techniques to retain a highly pure peptide product.
Part No: P180Issued year: 2018File size: 0.35mbFile type: pdf
In medicinal chemistry, organic synthesis will generate by-products with similar chemistry to the product. Separating these impurities from the product often becomes a challenge that often only prep HPLC can achieve.
In this poster, we discuss how using sample dry loading with a cation exchange scavenger media with flash chromatography
improves product purity by removing many of the impurities.
Part No: AN088.v1Issued year: 2014File size: 1.59mbFile type: pdf
Fragrance compounds, being invisible to UV, can be
elusive and difficult to isolate. In this application, a four
component mixture of fragrance compounds was purified
using Accelerated Chromatographic Isolation™ (ACI) and
identified by mass detection.
Part No: AN600Issued year: 2005File size: 0.08mbFile type: pdf
This Application Note reviews the use of ISOLUTE PPT+ plates for the isolation of drugs from biological fluids, including the method, recovery data and the impact of the filtrate’s cleanliness on analyte quantitation.
Part No: PPS321Issued year: 2013File size: 2.79mbFile type: pdf
Be sure you have your target compound the moment your purification is complete. The Isolera™
Dalton integrates the Biotage approach to flash chromatography with true compound identification
using mass detection.
Part No: AN079Issued year: 2014File size: 0.89mbFile type: pdf
Traditional RP-HPLC techniques not always critical because improvements in solid-phase peptide synthesis techniques over the years (microwave heating and ChemMatrix® solid phase supports) have reduced the burden on downstream purification steps. Mass directed flash purification can be a viable alternative to more expensive (and lower capacity) semi- or prep-scale RP-HPLC methods.
Part No: UI349Issued year: 2017File size: 0.61mbFile type: pdf
The tasks carried out during the maintenance visit shall include
servicing the listed components and a final check which is
based on factory acceptance and qualifications tests. These
two procedures constitute a Biotage Preventive Maintenance
Isolera Dalton identifies compounds by mass in real time during flash separation, leading to greater confidence in purification, higher purity, greater recovery, and a significant saving in time. Reduce or eliminate the need for time consuming off-line mass analysis during development programs, increase throughput and effectiveness for both chemists and analytical groups.
The Isolera ELSD (evaporative light-scattering detector) is a universal detector designed for use with Isolera flash purification systems when purifying organic compounds that
are undetectable UV or visible light. Flash chromatography with detection and fractionation is now possible when purifying carbohydrates, steroids, lipids, terpenes and other UVtransparent compounds.
Part No: TN/UG-0028.0509Issued year: 2009File size: 0.39mbFile type: pdf
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
Part No: TN-0024.0210 (2)Issued year: 2010File size: 0.61mbFile type: pdf
TheBiotage Isolera™LS development-scale flash purification
systemdramatically shortens large-scale purification run-times
with flow rates of 50-500mL/minute. Simply select or create a
method, load your sample, and run – it’s that easy. New
advanced features include: solvent-savingGradientOptimization
(GO), the ability to collect fractions on two separatewavelengths,
use of up to four solvents in a single gradient and add a third
With the Biotage Isolera Spektra, new, intelligent, time-saving features such as gradient optimization, λ-All peak detection, and real-time photodiode array (PDA) scanning enable chemists to purify more compounds in less time and reduce post-process purity analysis.
Part No: PPS314Issued year: 2013File size: 0.46mbFile type: pdf
A new Biotage innovation allows organic chemists to deliver high purity
compounds in the minimum amount of time using minimum amounts of solvent.
Chemists simply enter TLC data and follow the Isolera™ Spektra software’s
method recommendations, based on knowledge gained over 20 years of
experience in automated flash chromatography leadership.
Part No: PN413Issued year: 2003File size: 0.03mbFile type: pdf
Traditional liquid-liquid extraction, carried out in vials, can be difficult to automate. ISOLUTE Array
HM-N plates for supported liquid extraction offer an easy to automate alternative to this popular sample
preparation technique, eliminating the need to physically separate immiscible layers. Each step is a
simple liquid transfer or addition, and no mixing is required.
Part No: Issued year: 2008File size: 0.07mbFile type: pdf
This method selection guide is designed to minimize the choices required to optimize the Supported Liquid Extraction method. The buffer and solvent suggestions provide a range of pHs and solvent polarities for acidic, neutral and basic drugs of varying pKa and logP values. The selections are based on extensive work in Biotage’s R&D Laboratories.
This guide describes how to develop supported liquid extraction methods using ISOLUTE® SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted
Part No: TN417Issued year: 2007File size: 0.1mbFile type: pdf
The introduction of parallel synthesis in pharmaceutical R&D has resulted in the need for purification systems
that allow for increased throughput. With reactions being carried out in a microplate footprint, it is important that the reaction workup can also be performed in this way.When using the 96-well format, there are limitations on both the reaction scale and also the sorbent capacity for the purification step. Using a 24-well system allows for greater sorbent masses to be utilized, increasing the scale of purification.
Part No: TN-0025.0710Issued year: 2010File size: 0.32mbFile type: pdf
ISOLUTE® ENV+ is a hyper-cross linked polystyrene polymer sorbent capable of extracting a wide range of polar and water soluble analytes from a variety of matrices. The optimized surface area, pore structure and absence of fines makes ISOLUTE ENV+ a highly effective and efficient solution to the traditionally difficult extraction of very polar analytes. ISOLUTE ENV+ enables high recoveries at high flow rates making it the SPE column of choice for a variety of environmental and agrochemical applications.
Part No: TN109Issued year: 2004File size: 0.07mbFile type: pdf
This technical note includes a general discussion on non-polar sorbents (page 1), specifics on the use of ISOLUTE ENV+ for the extraction of polar analytes from aqueous samples (page 2), and ordering information (page 5).
Part No: TN142Issued year: 2009File size: 0.26mbFile type: pdf
This Chemistry Data Sheet provides procedures for both the automated and manual fractionation of EPH in soil. The
automated procedure is described on page 2 and the manual procedure is outlined on page 3. Chromatograms
illustrate the ability of ISOLUTE EPH to provide efficient fractionation of hexane or pentane soil extracts into
aliphatic and aromatic fractions (see Appendix).
Part No: TN411Issued year: 2007File size: 0.12mbFile type: pdf
Sample preparation techniques such as protein precipitation, supported liquid extraction or non-polar SPE may not be selective enough to give extracts of sufficient purity for low level analysis. In these cases, the selective mixed-mode approach to the extraction of basic and acidic drugs is a suitable alternative, giving very high purity extracts with minimal levels of co-extracted material.
Part No: PPS318Issued year: 2013File size: 2.05mbFile type: pdf
ISOLUTE® Myco SPE columns offer simple and efficient multiple mycotoxin sample preparation from a wide range of matrices, ideally suited for selective and fast LC-MS/MS analysis. ISOLUTE Myco SPE columns contain a novel polymer-based sorbent designed specifically to be selective enough to isolate a wide variety of different mycotoxins. One product with multiple applications, simplifying and streamlining your mycotoxin analysis procedures.
Part No: TN412Issued year: 2007File size: 0.09mbFile type: pdf
Non-polar SPE is commonly used for extraction of acidic, neutral and basic drugs from biological fluids. It is particularly useful when a parent drug and metabolites of different functionalities are to be extracted simultaneously, and will provide cleaner extracts than alternative sample preparation techniques such as protein precipitation.
Part No: PPS335Issued year: 2013File size: 0.99mbFile type: pdf
ISOLUTE PLD+ plates remove >99 % of plasma proteins and phospholipids, the main causes of ion suppression, leading to cleaner extracts and increased sensitivity (signal-to-noise
(S/N)) for a broad range of analytes.
Part No: PN424Issued year: 2007File size: 0.15mbFile type: pdf
Protein precipitation is a routinely used sample preparation technique for removal of proteins from biological fluid samples prior to analysis. Protein precipitation in the 96-well format, using filtration for protein removal, is a high throughput alternative to the traditional centrifugation based technique.
Part No: TN-0020.0810Issued year: 2010File size: 1mbFile type: pdf
QuEChERS (pronounced “catchers”) stands for Quick, Easy, Cheap, Effective, Rugged, and Safe. The technique is designed to be simple and involve a minimum number of steps; but still allow for effective clean up of complex samples. The QuEChERS technique involves two stages.
Part No: PPS373.V.1Issued year: 2016File size: 0.91mbFile type: pdf
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to a class of strong supported acids. As a bound sulfonic acid, it has a natural propensity to bind alkali and some transition metal group metals, which means that it can be used as a scavenger for metals with a +I or +II oxidation state, such as Na, K, Li, also
Pd, Rh, and Ru.
Part No: PPS372.V.1Issued year: 2016File size: 2.4mbFile type: pdf
ISOLUTE® Si-Trisamine is a silica bound equivalent of a trisamine base and can scavenge a variety of electrophiles, including aldehydes, acid chlorides, sulfonyl chlorides, isocyanates, isothiocyanates and heavy metal ions such as Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Pb+2, Ru+2 and Zn2+.
Part No: TN0017.0406Issued year: 2006File size: 0.05mbFile type: pdf
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to the class of strong acids. Similar
to ISOLUTE Si-TsOH (SCX-3)1, it can be used to (a) scavenge amines and other bases such
as anilines and borohydrides (b) as an acid catalyst in reactions or (c) as a replacement
for aqueous or organic acids in quenching reactions. The advantage of using SCX-2 as a
bound acid reagent is in that it eliminates work-up. The crude reaction mixture can be evaporated to dryness and the resulting free flowing solid can be purified directly by flash column chromatography.
Part No: TN0018.0406Issued year: 2006File size: 0.09mbFile type: pdf
ISOLUTE® Si-Thiol is the silica-bonded equivalent of 1-propanethiol, which is useful
for covalent scavenging of alkyl, benzyl, and allyl halides as well as a variety of other
electrophiles including acid chlorides and isocyanates. In addition it can be used to
scavenge a variety of metals1 used in organic synthesis including Pd, Pt, Cu, Hg, Ag and Pb.
Part No: PPS374.V.1Issued year: 2016File size: 0.98mbFile type: pdf
ISOLUTE® Si-Thiol is the silica-bound equivalent of 1-propanethiol which is primarily used in the pharmaceutical industry as a metal scavenger for metals such as Pd, Pt, Cu, Hg, Ag and Pb but it can also be useful for covalent scavenging of alkyl, benzyl and allyl halides as well as a variety of other electrophiles including acid chlorides and isocyanates.
Part No: TN0016.0406Issued year: 2006File size: 0.07mbFile type: pdf
ISOLUTE® Si-TsOH (SCX-3) is the bound equivalent of p-toluene sulfonic acid with a
pKa <1. Similar to ISOLUTE propylsulfonic acid (SCX-2)1 , it can be used to (a) scavenge
amines and other bases such as anilines and borohydrides (b) as an acid catalyst in reactions
or (c) as a replacement for aqueous or organic acids in quenching reactions.
Part No: PPS431.v1Issued year: 2016File size: 0.48mbFile type: pdf
At Chiba University’s Graduate School of Medicine and its affiliated hospital, researchers are developing an LC-MS/MS method for vitamin D analysis using ISOLUTE® SLE+ and other Biotage products. We interviewed Professor Mamoru Satoh at the Disease Proteomics Center and Mr. Takashi Ishige from the Inspection Department at the affiliated hospital.
Part No: TN-0011.0708Issued year: 2008File size: 0.34mbFile type: pdf
ISOLUTE® SLE+ Supported Liquid Extraction Plates offer an efficient alternative to traditional liquid-liquid extraction
(LLE) for bioanalytical sample preparation, extracting up to 400 μL of aqueous sample. ISOLUTE SLE+ plates provide
high analyte recoveries, eliminate emulsion formation, and cut sample preparation time in half.
Part No: TN-0012.0610 (2)Issued year: 2010File size: 0.35mbFile type: pdf
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion
formation and reduces sample preparation time by half.
Part No: AN068Issued year: 2013File size: 0.6mbFile type: pdf
The ability to synthesize peptides in a range of different
scales is a requirement in many research laboratories. The
Biotage® Initiator+ Alstra™ fully automated microwave peptide
synthesizer has a scale range of 5 μmol up to 2 mmol using
three different reactor vial sizes (5 mL, 10 mL and 30 mL).
The acyl carrier protein fragment, ACP (65–74), H-Val-Gln-
Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is
a well-known so-called difficult sequence and is commonly
used to evaluate the performance of new synthesis reagents
Here we demonstrate a variable scale synthesis on the
Initiator+ Alstra using the ACP (65–74) fragment as a model
Part No: PPS397Issued year: 2016File size: 0.23mbFile type: pdf
Customer Case: Satori Pharmaceuticals. Extracting target compounds from large volumes of mixture confronts chemists with challenges which are right at the heart of large scale process chemistry. Ruichao Shen at Satori Pharmaceuticals knows exactly what it takes.
Part No: P115Issued year: 2014File size: 2.69mbFile type: pdf
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Part No: P173Issued year: 2018File size: 0.55mbFile type: pdf
Urine drug testing to support pain management is a mainstay of the clinical toxicology laboratory. Reduced reimbursement has continued to put increasing pressure on laboratories. Many toxicology labs are moving to larger drug panels to increase throughput and efficiency, while reducing turn-around-time and cost.
Part No: PPS526Issued year: 2015File size: 0.68mbFile type: pdf
In the laboratory many chemicals are used in small and larger volumes. Table 1 shows several of the typical kinds of analyses done for environmental compliance. Semivolatile organic compound extraction and evaporation can be a large source of solvent emission to the atmosphere if the solvent is allowed to go up the hood and is not recaptured.
Part No: AN064Issued year: 2012File size: 1.09mbFile type: pdf
This application note describes the use of ISOLUTE® HM-N as a support material
for pre-adsorption of a reaction mixture prior to flash chromatography. This
approach is useful when loading reaction mixtures that are only soluble in polar
solvents onto a Biotage® SNAP Ultra, SNAP KP-SIL or SNAP KP-NH column.
Part No: AN128Issued year: 2019File size: 0.27mbFile type: pdf
In this application note, we compare the amounts of a threecomponent
mixture that can be separated on columns of the
same size, 25 g, but with different Silica material: SNAP KP-Sil
(average 50 μm irregular particles), Sfär Silica (60 μm spherical
particles) and Sfär Silica HC (20 μm spherical particles) using a
Biotage® Selekt instrument.
Part No: P211Issued year: 2019File size: 1.16mbFile type: pdf
This poster compares manual SPE (EVOLUTE EXPRESS CX) processing using the Biotage Pressure+ 96 with automated processing using Biotage Extrahera, using a suite of 98 drugs of abuse analytes in whole blood . Parameters such as the total time for extraction of 96 samples, and consistency of analyte recovery and RSD are explored.