Part No: P142Issued year: 2016File size: 0.57mbFile type: pdf
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Part No: AN023-HORIssued year: 2012File size: 0.85mbFile type: pdf
The purpose of this application note is to ensure that your environmental laboratory is in compliance with EPA Method 1664A Modifications or EPA Method 1664B utilizing the Horizon Technology SPE-DEX® 1000XL/3000XL Controller with application firmware version 1.08.
Part No: AN024-HORIssued year: 2012File size: 0.86mbFile type: pdf
The purpose of this application is to demonstrate the viability of a solid phase extraction (SPE) method utilizing the Horizon Technology SPE-DEX 1000/3000XL Controller with application firmware version 2.2 to extract samples for EPA Method 1664A/B and fulfill all QC requirements.
Part No: AN025-HOR.V.1Issued year: 2012File size: 0.88mbFile type: pdf
The purpose of this application note is to demonstrate the
viability of a solid phase extraction (SPE) method utilizing
the Biotage® Horizon 4790 Extractor with the Envision® Platform software version 1.02 to extract samples for EPA Method 1664A/B and fulfil all QC requirements.
Part No: AN879Issued year: 2017File size: 0.89mbFile type: pdf
There are a wide range of volatile and semi-volatile contaminants finding their way into both terrestrial bodies and water sources worldwide. In the United States (US), the contaminants are analyzed
according to stipulated US-EPA methods. In the European Union (EU), a large number of these same compounds are tested according to the European Water Framework Directive. Though these analytes are approached differently from a regulatory perspective, it is clear that background monitoring occurs on a global basis. Initial extraction of these analytes depends on the matrix being analyzed and is often a multifaceted process, but ultimately analysts are presented with some form of extraction/organic solvent they must concentrate to achieve instrumental limits of quantification. Presented within this technical note are the results of such an evaporative process using the new Biotage TurboVap® II.
Part No: AN050-HORIssued year: 2009File size: 1.23mbFile type: pdf
Polychlorinated biphenyls (PCBs) are found throughout the environment and are recognized as a serious health threat. They accumulate in animals and plants and become more concentrated as they progress up the food chain. PCBs can be found in the fatty tissues of human populations in all industrial countries, and they can be passed from mother to fetus through the placenta and to an infant through breast milk. They have been introduced into the environment mainly through the use of Aroclor products.
Part No: AN086-HORIssued year: 2013File size: 0.76mbFile type: pdf
Natural and synthetic phenolic compounds have been used in a wide range of applications, from antiseptics and fungicides, to food additives. With the adoption of the Clean Water Act (CWA), many phenolic compounds were placed on a list of Priority Pollutants.
Part No: UI404Issued year: 2014File size: 0.24mbFile type: pdf
Although the general analysis process contains extraction, extract drying, and evaporation steps prior to chromatography, this note will describe a procedure for the concentration of organic solvent extracts containing phenolic compounds.
Part No: PPS430Issued year: 2016File size: 0.97mbFile type: pdf
At the Forensic Medicine Lab at Toho University, researchers use ISOLUTE® SLE+ columns from Biotage. When dealing with samples that easily form emulsions like urine or blood, it allows researchers to use the established liquid-liquid extraction technique, saving significant amount of time on analysis. We spoke with the Head of the Forensic Medicine Lab Professor Masaru Terada.
Part No: P220Issued year: 2020File size: 0.44mbFile type: pdf
Evaporative crosstalk is well-to-well cross contamination
during extract evaporation after SLE or SPE extraction in a 96
well plate. It is often observed with volatile analytes like
methamphetamine and amphetamine, but it can also occur
with other drugs and metabolites when urine samples have
very high analyte concentrations. ASMS, 2020.
Part No: UI406Issued year: 2016File size: 0.33mbFile type: pdf
The SPE-DEX 3100 Extractor is controlled through a small hand-held controller tethered to the instrument. The color touch screen is easy to see from any angle and features large icons to show the status of each station in the unit at any time.
Part No: Issued year: 2009File size: 0.12mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB and a number of acquisitions were performed within the Medicinal Chemistry sector during the years of 2003-2005. After divesting the business area Biosystems in October 2008, the Company’s business is today made up entirely of the business area Discovery Chemistry. Biotage’s headquarters is located in Uppsala.
Part No: Issued year: 2010File size: 0.2mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (”the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2011File size: 0.72mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (“the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2013File size: 0.11mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company is mainly active within analytical and organic chemistry.
Part No: PPS445Issued year: 2017File size: 0.62mbFile type: pdf
Ease of use is what stands out as the top feature of Isolera™ flash chromatography
system for Professor Anna Bernardi, head of the synthetic organic chemistry
research group at the University of Milan. Her current research goal: developing
sugar-like molecules, called glycomimetics, for healthier life.
Part No: PPS443.V.1Issued year: 2019File size: 2.98mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: Issued year: 2011File size: 0.07mbFile type: pdf
Through innovative patented technology based on molecularly imprinted polymers (MIPs)
you will experience improved clean up in your processes. With a high probability of success,
unwanted contaminants or high value desirables can be selectively extracted from your
processes, resulting in more efficient production and cleaner products.
Part No: P102Issued year: 2014File size: 0.6mbFile type: pdf
Designed polymers are a class of selective resins with engineered selectivities for particular target molecules or ‘classes’ of molecules. These designed polymers are obtained by careful tuning of their surface chemistry and morphology which allows them to exhibit unique separation capabilities. The tailored and optimized selectivity of designed polymers is utilized to conduct difficult separations that are not able to be accomplished with conventional separation resins or other techniques.
Part No: AN933Issued year: 2020File size: 2.25mbFile type: pdf
This application note describes the extraction of a panel of estrogenic hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 5% for all analytes. Linearity of greater than 0.99 is achieved for Estrone (E1) and Estradiol (E2) from 5–2000 pg/mL, and 50–20000 pg/mL for Estriol (E3). No derivatization is required, and detection limits are enhanced using a fluorinated mobile phase.
Part No: P216.V.2Issued year: 2020File size: 2.46mbFile type: pdf
Monitoring estrogenic compounds such as Estrone (E1), Estradiol
(E2), and Estriol (E3) in human serum is essential in clinical research and diagnostics. Detection in men, post-menopausal women and children requires very low limits of detection (LODs) which can be challenging.
Part No: AN927Issued year: 2019File size: 1.18mbFile type: pdf
This application note demonstrates the performance of the
Biotage® Horizon 5000 automated solid phase extraction system
for extraction of 1,4-dioxane from drinking water, in compliance with US EPA Method 522.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: AN053-HORIssued year: 2015File size: 1.3mbFile type: pdf
Carbaryl, a commonly used insecticide for the past 20 years, has become a significant environmental concern throughout the world. Also known through its trademarked name Sevin, it is a cholinesterase inhibitor and can be toxic to humans, causing problems in the blood, nervous, and reproductive systems.