Part No: AN078-HORIssued year: 2015File size: 1.25mbFile type: pdf
This application note was developed to demonstrate the extraction of five organophosphate compounds Monocrotophos, Diazinon, Malathion, EPN, and Methamidophos using one solid phase extraction method with one pre-treatment step of sodium chloride (NaCl). The method uses the Biotage® Horizon 4790 automated SPE extraction system. It will show the efficiency of the extraction while demonstrating excellent recoveries of OPP compounds using methylene chloride and minimal amounts of acetone after sample pre-treatment with sodium chloride. Methods were developed and results are shown using 47 mm Atlantic® HLB-H disks and carbon cartridges.
Part No: P201Issued year: 2019File size: 0.9mbFile type: pdf
An extraction protocol using a 10 mg mixed-mode cation exchange sorbent (EVOLUTE® EXPRESS CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving microelution off of the sorbent requiring no evaporation or reconstitution steps were also developed.
Part No: AN009-HOR.V.1Issued year: 2015File size: 0.58mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN108-HORIssued year: 2016File size: 2.35mbFile type: pdf
Drinking water is a critical resource important for human health. As water becomes more scarce, quality will become increasingly important and monitoring will be required more frequently and at lower concentration levels. The number of compounds and lower concentrations for monitoring will make extraction and analysis even more challenging.
Part No: AN019-HORIssued year: 2010File size: 0.92mbFile type: pdf
The City of Fort Worth, Water Department conducted an evaluation of the Atlantic™ solid phase extraction (SPE) C18 disks using EPA Method 608. This application note describes the procedure to determine low ppb levels of organochlorine pesticides and PCBs in wastewater. This procedure used methylene chloride as the primary extraction solvent, followed by a hexane solvent exchange. Extracts were then analyzed by GC/ECD using a pressure pulse injection technique.
Part No: AN021-HORIssued year: 2009File size: 0.89mbFile type: pdf
Method 1657 describes the procedure to determine low ppb levels of organophosphorus pesticides in municipal and industrial wastewater.
The City of Fort Worth Water Department implemented Automated SPE for the analysis of organophosphorous pesticides by EPA Method 1657, using the Atlantic™ C18 solid phase extraction disk.
The Biotage® Horizon 4790 Automated Extraction System with Envision®
Platform controller, and the DryVap® Automated Drying
and Concentration System were used in this application note.
Part No: AN072-HORIssued year: 2012File size: 0.88mbFile type: pdf
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants, naturally occurring in coal, crude oil, gasoline, and their byproducts (e.g. coal tar or creosote). In addition, PAHs are formed in the incomplete combustion processes of all organic materials, such as wood or fossil fuels. Consequently, the EU water framework directive (WFD) lists in its annex X the whole group of PAHs as priority hazardous substances.
Part No: AN038-HORIssued year: 2009File size: 2.13mbFile type: pdf
This application note demontrates that the Biotage® fully automated extraction, drying and concentration systems used with the Atlantic™ HLB disk are capable of extracting PAH compounds from sea water.
Typical extraction times using the Biotage® Horizon 4790 range from 20 to 25 minutes while drying and concentration on the DryVap® with the DryDisk® took approximately 35 minutes.
Part No: AN055-HORIssued year: 2015File size: 1.36mbFile type: pdf
Polybrominated diphenyl ethers (PBDEs) are a worldwide contamination problem. Structurally similar to PCBs, these compounds are long-lived in the environment and can bio-accumulate throughout the food chain. The health hazards of these chemicals have attracted increasing scrutiny and, as such, a great deal of research and regulations have been implemented to manage and control them.
Part No: AN011-HORIssued year: 2010File size: 0.93mbFile type: pdf
The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).
Part No: AN035-HORIssued year: 2009File size: 0.83mbFile type: pdf
The purpose of this application note is to demonstrate the use of a fully automated solid phase extraction (SPE) and concentration system that provides fast extraction while improving the quality and consistency of results for EPA Method 8270D.
Part No: AN104-HORIssued year: 2016File size: 1.48mbFile type: pdf
Drinking water is an important resource that can provide significant exposure to humans if it is polluted. Analysis of source water, which may come from surface water, groundwater or treated water must be evaluated for possible contaminants to ensure regulatory compliance.1 Drinking water at the tap is often evaluated for contaminants that may be introduced through leaks in the pipes or pipe materials.
Part No: AN036-HORIssued year: 2009File size: 0.86mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the Biotage® Horizon 4790 Automated Extractor System when used for the analysis of nitramine, nitroaromatic, and nitrate ester compounds in surface and ground water.
Part No: AN058-HORIssued year: 2010File size: 0.9mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the automated Horizon Technology solution when used for the analysis of EPA method 8061A phthalate ester compounds in surface and ground water. Method 8061A states gas chromatography/mass spectrometry (GC/MS) can be used as an alternative for compound confirmation for phthalate esters.
Part No: AN064-HORIssued year: 2015File size: 1.69mbFile type: pdf
Although not frequently a problem, recent incidents at sea have led to large amounts of crude oil being released and dispersed throughout the Gulf of Mexico, such as in the Deepwater Horizon Gulf oil spill of 2010. Originally presumed to be Louisiana Sweet Crude, sample testing later revealed a harsher form of crude containing a high amount of asphaltenes was actually being released.
Part No: AN063-HOR.V.1Issued year: 2012File size: 0.22mbFile type: pdf
Oftentimes it becomes necessary to test sea water for oil and grease analytes. Whether it is due to a controlled release of effluent water from a waste water treatment plant (usually during large rain storms) or a large oil spill, there is a need generated for a reliable, automated extraction and evaporation system.
Part No: AN061-HORIssued year: 2010File size: 0.74mbFile type: pdf
Throughout the world, a lack of regulation has been blamed for the detection of pharmaceuticals and personal care products (PPCP) in some bodies of water. While the detected amounts are usually relatively small, the persistence of these compounds means that they can cause untold damage to the ecosystem for years to come.
Part No: AN065-HORIssued year: 2010File size: 2.07mbFile type: pdf
Although not frequently a problem, recent incidents at sea have led to large amounts of crude oil being released and dispersed throughout the Gulf of Mexico. Originally presumed to be Louisiana Sweet Crude, sample testing later revealed a harsher form of crude containing a high amount of asphaltenes was actually being released. The differences are substantial, as Louisiana Sweet degrades more readily in nature than crude oil containing asphaltenes.
Part No: Issued year: 2014File size: 0.12mbFile type: pdf
This poster describes the development and validation of a method for supported liquid extraction of serum cortisol, with analysis by UPLC-MS/MS. The aim of this study was to develop a candidate reference method that could then be used to underpin the UK NEQAS Cortisol scheme.
MSACL EU 2014
Part No: AN004-HORIssued year: 2009File size: 0.72mbFile type: pdf
This evaluation study was conducted to determine the feasibility of the Biotage SPE-DEXÒ 4790 Automated Extraction System for the extraction of herbicides and pesticides. The preliminary results demonstrate the capability of this method for the automated SPE extraction of organic compounds.
Part No: Issued year: 2017File size: 0.27mbFile type: pdf
This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX).
Part No: AN057Issued year: 2012File size: 0.21mbFile type: pdf
The formation of diarylethers by reacting an arylhalide and phenol is usually a reaction demanding long reaction times, high temperatures and strong bases, in order to obtain acceptable yield. The substitution patent of the electrophile and the nucleophile affects the reaction times mostly. A sterically hindered electrophile and a strongly deactivated nucleophile as outlined in the (Scheme 1) below, gives a very low yield (13 %) at conventional reflux for 2 weeks.1,2 Remainder was
recovered starting material. We have previously reported the dramatically shortened reaction time to 1 hour along with improved yield running the reaction outlined in the Scheme by heating by microwaves.
Part No: BIOMARK97.3Issued year: 2019File size: 0.24mbFile type: pdf
This letter is to inform you that effective April 1st, 2019 Biotage
will phase out the following products:
1. Biotage ZIP®, all column sizes as reported in Table 1
2. Biotage ZIP® Sphere, all column sizes as reported in Table 2
Part No: P131Issued year: 2015File size: 0.47mbFile type: pdf
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Part No: AN101-HORIssued year: 2016File size: 1.07mbFile type: pdf
This application note highlights a SPE disk which was specifically designed to increase the recoveries of traditionally difficult polar compounds as well as help to increase the diversity of the traditional 525.2 list to include those compounds which are of concern in today’s society.
Part No: P171Issued year: 2017File size: 0.69mbFile type: pdf
This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids.
SOFT 2017, Boca Raton
Part No: P174Issued year: 2017File size: 1.44mbFile type: pdf
This poster discusses the potential for a single extraction protocol
for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis.
SOFT 2017, Boca Raton
also presented at SFTA, Marseille, France, 2018
Part No: P157Issued year: 2017File size: 0.8mbFile type: pdf
This poster demonstrates that a large urine panel, comprised of 43 DOAs, from multiple drug classes, can be simultaneously screened by mixed-mode cation exchange SPE (using EVOLUTE EXPRESS CX 96 well plates) despite their disparate intermolecular traits, by thoughtfully selecting appropriate organic wash and elution conditions that simultaneously enable sample isolation and detection along with minimizing sample matrix effects.
The extraction method is automated using the Biotage® Extrahera™ Automated sample Preparation Platform.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Part No: DLV_TN.0111Issued year: 2011File size: 0.08mbFile type: pdf
One of the most common flash purification challenges is
dealing with hard-to-dissolve crude samples. Because polar
solvents cause poor chromatographic results when used as
injection solvents in normal-phase flash chromatography, other
sample load options are needed.
Part No: prodes57Issued year: 2019File size: 0.32mbFile type: pdf
The DryDisk® 50 mL Barrel (PN 40-2485) is a disposable sample preparation
device that provides a fast and simple approach for removing
residual water from non-polar solvents. The DryDisk membrane replaces
the conventional sodium sulfate (Na2SO4) drying technique.
Part No: PPS515Issued year: 2019File size: 0.23mbFile type: pdf
The DryDisk membrane drying system provides advantages for removing water from nonpolar solvents, important in protecting the chromatograph in the analysis step, especially critical in protecting GC and GC/MS.
Part No: UI405Issued year: 2015File size: 0.46mbFile type: pdf
Removing water with a membrane rather than the older technique of passing the solvent through a column of sodium sulfate (Na2SO4) brings several advantages. The most important analytically is that the membrane will not adsorb analytes or contaminate the extract with matrix or other potential interferences.
Part No: PPS539Issued year: 2019File size: 0.25mbFile type: pdf
Why spend hours using traditional evaporation techniques when the automated evaporation capability of the DryVap System can take your compound to
dryness in minutes? Through the precise application of vacuum, heat and nitrogen sparge, the DryVap System gently and predictably evaporates all residual solvent from your compound allowing you to quickly move on to the next step.
Part No: P149Issued year: 2016File size: 0.12mbFile type: pdf
With the de-criminalization of recreational cannabis, containing the hallucinogen THC, and other cannabinoids with purported medicinal value, e.g. CBD (Figure 1), in several states a need for higher purity “products” has become a necessity. Current technology uses extraction with supercritical fluids or other non-supercritical solvents to remove the products of interest from other endogenous species such as lipids, terpenes, and chlorophylls as well as pesticides.
These techniques help clean up raw extracts and isolate cannabinoids with higher-purity but not to the levels desired by many producers so there is a developing need for a secondary purification step.
Part No: PPS376Issued year: 2015File size: 1.6mbFile type: pdf
User Case: Kissei Pharmaceutical Co., Ltd. One of Japan’s innovative pharmaceutical companies uses Isolera™ flash
purification systems and Biotage® Initiator+ microwave synthesizers in the
development of new prescription drugs. Modern lab instruments contribute to
efficient use of time and resources at Kissei Pharmaceuticals.
Part No: P143Issued year: 2016File size: 0.27mbFile type: pdf
Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation.
This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine (using EVOLUTE EXPRESS CX), oral fluid (using EVOLUTE EXPRESS CX) and whole blood (using ISOLUTE SLE+).
Part No: PN43Issued year: 2011File size: 0.27mbFile type: pdf
Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal.
Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011
Part No: P078Issued year: 2014File size: 0.25mbFile type: pdf
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Part No: P032Issued year: 2011File size: 1.35mbFile type: pdf
Endogenous phospholipids (outline structure shown in
Figure 1.) present in biological fluids are a major problem
in LC-MS/MS analysis. Due to their strong retention
characteristics in reversed phase chromatography
phospholipids tend not to elute as discrete peaks and are
often very difficult to separate from analytes of interest.
This co-elution often leads to areas of suppression or enhancement in the chromatogram which in turn can cause
quantitation issues. Supported liquid extraction (SLE) is an
analogous technique to traditional liquidliquid
extraction. This poster compares phospholipid removal using a wide variety of solvent combinations, pH control and polar extraction solvents on supported liquid extraction plates
Part No: AN096-HORIssued year: 2014File size: 2.89mbFile type: pdf
Mycotoxin testing in consumer food products has become increasingly important as global food trade increases, making it necessary to identify mycotoxins efficiently and accurately. Deoxynivalenol, also known as vomitoxin, is a mycotoxin commonly found in wheat feed crops. Because wheat is a highly-used raw agricultural export commodity in many counties for both animal feed and consumer food products, it is an important component in the diets of both humans and animals.
Part No: Issued year: 2011File size: 1.65mbFile type: pdf
User Report: Syro I, University of Tokyo. The Suga Laboratory at RCAST, University of Tokyo, uses the Biotage Syro I automated peptide synthesizer for scaling up the chemical synthesis of non-standard peptides.