Part No: PN43Issued year: 2011File size: 0.27mbFile type: pdf
Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal.
Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011
Part No: P078Issued year: 2014File size: 0.25mbFile type: pdf
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Part No: P032Issued year: 2011File size: 1.35mbFile type: pdf
Endogenous phospholipids (outline structure shown in
Figure 1.) present in biological fluids are a major problem
in LC-MS/MS analysis. Due to their strong retention
characteristics in reversed phase chromatography
phospholipids tend not to elute as discrete peaks and are
often very difficult to separate from analytes of interest.
This co-elution often leads to areas of suppression or enhancement in the chromatogram which in turn can cause
quantitation issues. Supported liquid extraction (SLE) is an
analogous technique to traditional liquidliquid
extraction. This poster compares phospholipid removal using a wide variety of solvent combinations, pH control and polar extraction solvents on supported liquid extraction plates
Part No: AN096-HORIssued year: 2014File size: 2.89mbFile type: pdf
Mycotoxin testing in consumer food products has become increasingly important as global food trade increases, making it necessary to identify mycotoxins efficiently and accurately. Deoxynivalenol, also known as vomitoxin, is a mycotoxin commonly found in wheat feed crops. Because wheat is a highly-used raw agricultural export commodity in many counties for both animal feed and consumer food products, it is an important component in the diets of both humans and animals.
Part No: Issued year: 2011File size: 1.65mbFile type: pdf
User Report: Syro I, University of Tokyo. The Suga Laboratory at RCAST, University of Tokyo, uses the Biotage Syro I automated peptide synthesizer for scaling up the chemical synthesis of non-standard peptides.
Part No: AN026-HORIssued year: 2012File size: 0.85mbFile type: pdf
The purpose of this application note is to introduce a modification to the preprogrammed methods on the Biotage® Horizon 1000/3000XL Controller with application firmware version 2.2. The methods are utilized in the solid phase extraction (SPE) process to extract samples for EPA Method 1664AB and fulfill QC requirements.
Part No: 412941-DIssued year: 2012File size: 1.18mbFile type: pdf
ELSD-1080 (evaporative light-scattering detector) is a
universal detector designed for use with Isolera One
and Isolera Four systems when purifying compounds
with little or no UV absorption such as carbohydrates,
steroids, lipids, and terpenes
Part No: AN060-HORIssued year: 2010File size: 1.33mbFile type: pdf
This purpose of this study is to determine how the Directto- GC vial concentrator tube and the DryVap® Concentrator System can optimize the recoveries for both low and high boiling point semi-volatile compounds. A spiking mixture from EPA method 8270D was chosen due to the more volatile compounds in the mix that are difficult to retain during the concentration and subsequent rinsing process; while the higher boiling compounds are more likely to adsorb onto the glass walls.
Part No: AN084Issued year: 2014File size: 0.86mbFile type: pdf
This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.
Part No: AN028-HORIssued year: 2010File size: 0.8mbFile type: pdf
Method 1668A is used for determining the level of chlorinated biphenyl congeners by high resolution gas chromatography with high resolution mass spectrometry (HRGC/HRMS). For aqueous samples (samples containing less than 1% solids) – Stable isotopically labelled analogs of the toxic PCB’s are spiked into a 1L sample, and the sample is extracted using the Biotage® Horizon 4790 Automated Extraction System along with the Atlantic™ C18 solid phase extraction (SPE) disk.
Samples are then dried and concentrated using the DryVap® Automated Drying and Concentrating System with DryDisk® technology.
Part No: AN005-HORIssued year: 2009File size: 0.8mbFile type: pdf
EPA Method 506 is used to determine Phthalate and Adipate Esters in drinking water. The analytes are extracted from water using a Horizon Technology 47 mm Atlantic C18 disk. The disk is extracted with Acetonitrile and Methylene Chloride. The extract is then dried and concentrated to a final volume of 1.0 mL using the Horizon Technology DryVap® with DryDisk® technology. Final analysis is by GC/PID.
Part No: AN006-HORIssued year: 2009File size: 0.8mbFile type: pdf
Method 508.1 is used to determine twenty-nine chlorinated
pesticides, three herbicides, and four organohalides in
ground water, drinking water, and water in any treatment
stage. The analytes are extracted from the water using a 47
mm C18 disk. The disk is extracted on the Horizon
Technology SPE-DEX® 4790 Automated Extraction
System using Ethyl Acetate and Methylene Chloride
(DCM). The extract is then dried and concentrated using
the Horizon Technology DryVap® with DryDisk®
technology. Final analysis is by GC/ECD.
Part No: AN010-HORIssued year: 2009File size: 0.8mbFile type: pdf
Method 526 is a gas chromatography / mass spectrometry(GC/MS) method for the determination of selected semivolatile compounds in raw and finished drinking waters.This method efficiently extracts analytes using the SPEDEX® 4790 with an Atlantic® 47 mm polystyrene divinylbenzene (DVB) disk. The disk is extracted with using Ethyl Acetate (EtAc) and Methylene Chloride (DCM). The extract is then dried and concentrated using the DryVap® concentrator system coupled with DryDisk® technology to a final volume of 1.0 mL. Final analysis is done by GC/MS.
Part No: AN013-HORIssued year: 2009File size: 0.74mbFile type: pdf
EPA Method 548.1 details the procedure for the determination of endothall in drinking water by ionexchange solid phase extraction (SPE), acidic methanol methylation, and gas chromatography / mass spectrometry.
This note presents data from the State of Idaho Bureau of Labs for the initial demonstration of capability using the Biotage® Horizon T4790 Automated Extractor System.
Part No: AN015-HORIssued year: 2009File size: 0.79mbFile type: pdf
EPA Method 552.1 is an ion exchange procedure used for determining haloacetic acids and dalapon in drinking water and drinking water sources.
The purpose of this application note is to present data demonstrating the capability of the Horizon Technology SPE-DEX® 4790 Automated Extraction System to perform the sample extraction required for this method.
Part No: P128Issued year: 2015File size: 0.26mbFile type: pdf
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
Part No: P126Issued year: 2015File size: 0.48mbFile type: pdf
This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared.