With the de-criminalization of recreational cannabis, containing the hallucinogen THC, and other cannabinoids with purported medicinal value, e.g. CBD (Figure 1), in several states a need for higher purity “products” has become a necessity. Current technology uses extraction with supercritical fluids or other non-supercritical solvents to remove the products of interest from other endogenous species such as lipids, terpenes, and chlorophylls as well as pesticides. These techniques help clean up raw extracts and isolate cannabinoids with higher-purity but not to the levels desired by many producers so there is a developing need for a secondary purification step.

English Pharma Posters

User Case: Kissei Pharmaceutical Co., Ltd. One of Japan’s innovative pharmaceutical companies uses Isolera™ flash purification systems and Biotage® Initiator+ microwave synthesizers in the development of new prescription drugs. Modern lab instruments contribute to efficient use of time and resources at Kissei Pharmaceuticals.

Customer Cases

This poster demonstrates two fast and robust methods for the extraction of buprenorphine and norbuprenorphine from urine, oral fluid and whole blood. SOFT 2015

96-well plate Buprenorphine Clinical Column English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pharmaceuticals Posters Saliva Urine Whole blood

Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine (using EVOLUTE EXPRESS CX), oral fluid (using EVOLUTE EXPRESS CX) and whole blood (using ISOLUTE SLE+). MSACL 2016

96-well plate Buprenorphine Clinical Column English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pain Management Posters Saliva Synthetic urine Urine Whole blood

Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal. Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011

Application Notes Aqueous Biological fluids Column LC-MS/MS Plasma RapidTrace Serum Urine Whole blood

This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts. HPLC 2014

96-well plate Clinical English Forensic LC-MS/MS Pharma Posters

Endogenous phospholipids (outline structure shown in Figure 1.) present in biological fluids are a major problem in LC-MS/MS analysis. Due to their strong retention characteristics in reversed phase chromatography phospholipids tend not to elute as discrete peaks and are often very difficult to separate from analytes of interest. This co-elution often leads to areas of suppression or enhancement in the chromatogram which in turn can cause quantitation issues. Supported liquid extraction (SLE) is an analogous technique to traditional liquidliquid extraction. This poster compares phospholipid removal using a wide variety of solvent combinations, pH control and polar extraction solvents on supported liquid extraction plates ASMS 2011

English Posters

An efficient 2-step synthesis of bezafibrate from tryamine under microwave conditions has been developed using SmithReactionKitTM and Microwave Syntheses technology.

Application Notes

Mycotoxin testing in consumer food products has become increasingly important as global food trade increases, making it necessary to identify mycotoxins efficiently and accurately. Deoxynivalenol, also known as vomitoxin, is a mycotoxin commonly found in wheat feed crops. Because wheat is a highly-used raw agricultural export commodity in many counties for both animal feed and consumer food products, it is an important component in the diets of both humans and animals.

Application Notes Deoxynivalenol (DON) English Food Food HPLC Mycotoxin SmartPrep

User Report: Syro I, University of Tokyo. The Suga Laboratory at RCAST, University of Tokyo, uses the Biotage Syro I automated peptide synthesizer for scaling up the chemical synthesis of non-standard peptides.

Customer Cases

The purpose of this application note is to introduce a modification to the preprogrammed methods on the Biotage® Horizon 1000/3000XL Controller with application firmware version 2.2. The methods are utilized in the solid phase extraction (SPE) process to extract samples for EPA Method 1664AB and fulfill QC requirements.

Application Notes Disk EPA 1664 A Analytes EPA 1664 B Analytes English Environmental Gravimetric Oil & Grease Total Petroleum Hydrocarbons (TPH, SGT-HEM) Water

ELSD-1080 (evaporative light-scattering detector) is a universal detector designed for use with Isolera One and Isolera Four systems when purifying compounds with little or no UV absorption such as carbohydrates, steroids, lipids, and terpenes

Manuals & User Guides

Basically, my problem is this: the instrument seems incapable of eluting the solvent into the collection vial.

Tech Tips

This purpose of this study is to determine how the Directto- GC vial concentrator tube and the DryVap® Concentrator System can optimize the recoveries for both low and high boiling point semi-volatile compounds. A spiking mixture from EPA method 8270D was chosen due to the more volatile compounds in the mix that are difficult to retain during the concentration and subsequent rinsing process; while the higher boiling compounds are more likely to adsorb onto the glass walls.

Application Notes English Environmental GC-MS SVOC Semi-Volatile Organics Water

This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.

Application Notes

Method 1668A is used for determining the level of chlorinated biphenyl congeners by high resolution gas chromatography with high resolution mass spectrometry (HRGC/HRMS). For aqueous samples (samples containing less than 1% solids) – Stable isotopically labelled analogs of the toxic PCB’s are spiked into a 1L sample, and the sample is extracted using the Biotage® Horizon 4790 Automated Extraction System along with the Atlantic™ C18 solid phase extraction (SPE) disk. Samples are then dried and concentrated using the DryVap® Automated Drying and Concentrating System with DryDisk® technology.

4790 Application Notes EPA 1668A Analytes English Environmental GC-MS PCBs Water

EPA Method 506 is used to determine Phthalate and Adipate Esters in drinking water. The analytes are extracted from water using a Horizon Technology 47 mm Atlantic C18 disk. The disk is extracted with Acetonitrile and Methylene Chloride. The extract is then dried and concentrated to a final volume of 1.0 mL using the Horizon Technology DryVap® with DryDisk® technology. Final analysis is by GC/PID.

Adipate esters Application Notes Disk EPA 506 Analytes English Environmental Phthalates Phthalic acid esters

Method 508.1 is used to determine twenty-nine chlorinated pesticides, three herbicides, and four organohalides in ground water, drinking water, and water in any treatment stage. The analytes are extracted from the water using a 47 mm C18 disk. The disk is extracted on the Horizon Technology SPE-DEX® 4790 Automated Extraction System using Ethyl Acetate and Methylene Chloride (DCM). The extract is then dried and concentrated using the Horizon Technology DryVap® with DryDisk® technology. Final analysis is by GC/ECD.

Application Notes Chorinated Pesticides Drinking water English Environmental GC-MS Ground water Herbicides Organohalides Pesticides US EPA Method 508.1 Water

Method 526 is a gas chromatography / mass spectrometry(GC/MS) method for the determination of selected semivolatile compounds in raw and finished drinking waters.This method efficiently extracts analytes using the SPEDEX® 4790 with an Atlantic® 47 mm polystyrene divinylbenzene (DVB) disk. The disk is extracted with using Ethyl Acetate (EtAc) and Methylene Chloride (DCM). The extract is then dried and concentrated using the DryVap® concentrator system coupled with DryDisk® technology to a final volume of 1.0 mL. Final analysis is done by GC/MS.

Application Notes Disk Drinking water English Environmental GC-MS Semi-Volatile Organics

EPA Method 548.1 details the procedure for the determination of endothall in drinking water by ionexchange solid phase extraction (SPE), acidic methanol methylation, and gas chromatography / mass spectrometry. This note presents data from the State of Idaho Bureau of Labs for the initial demonstration of capability using the Biotage® Horizon T4790 Automated Extractor System.

Application Notes Disk Drinking water EPA 548.1 Analytes Endothall English Environmental GC-MS