This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of various drugs of abuse from hydrolyzed urine prior to LC-MS/MS analysis. drugs of abuse, sle, multiclass

2-Hydroxyethylflurazepam 7-Aminoclonazepam 96-well plate Alprazolam Amphetamine Amphetamines Anhydroecgonine methyl ester Application Notes Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clonazepam Cocaethylene Cocaine Cocaines Codeine Column Diazepam Ecgonine methyl ester Estazolam Fentanyl Flunitrazepam Hydrolysed urine Hydroxyalprazolam Hydroxytriazolam Ketamine Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Norcocaine Nordiazepam Norfentanyl Norketamine Opiates Opioid Oxazepam Temazepam Triazolam Zaleplone Zolpidem Zopiclone z-drugs

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of various drugs of abuse from non- hydrolyzed urine prior to LC-MS/MS analysis. drugs of abuse, sle, multiclass

2-Hydroxyethylflurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Application Notes Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clonazepam Cocaine Cocaines Codeine Diazepam Dihydrocodeine (DHC) EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Hydroxyalprazolam Hydroxytriazolam Ketamine LC-MS/MS MDA MDEA MDMA Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opioid Oxazepam Oxymorphone RapidTrace TECAN Temazepam Triazolam Urine Zaleplone Zolpidem Zopiclone z-drugs

This application note describes a SPE-UHPLC-MS/MS procedure for the analysis of 8-oxodG, a biomarker of oxidative stress, from various human biofluids using ISOLUTE ENV+ solid phase extraction columns. Oxidative stress is the imbalance between the production of reactive oxygen species (ROS) and the body’s ability to eliminate them, in favour of the former. ROS have been linked with development of numerous diseases including Parkinson’s and Alzheimer’s and have also been associated with fertility problems. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is one of the mutagenic nucleobase modifications produced in DNA and the 2’deoxyribonucleotide pool, by the reaction of ROS. Measurement of 8-oxodG in biological fluids can provide a useful indicator of oxidative stress. 8-oxodG, sample prep, ENV+, urine, biological fluids, biomarker, oxidative stress, nucleotide,

8-Oxo-7,8-dihydro-2'-deoxyguanosine 8-oxodG Application Notes Biomarker Clinical Column LC-MS/MS Urine

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite Testosterone

This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to analysis by UPLC-MS/MS. Optimised protocols for 200 uL and 500 uL sample volumes are included.

2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Amphetamine Amphetamines Application Notes Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Cathinones Clinical Clonazepam Cocaine Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Pain Management Phencyclidine Phencyclidine (PCP) Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to UPLC-MS/MS analysis. The sample preparation is optimised to minimise matrix effects due to the buffers used in the collection device. Estimated LOQs range from 0.1-1 ng/mL for the various analytes.

2-Hydroxyethylflurazepam 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Application Notes Basic drugs Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clinical Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Pharmaceuticals Phencyclidine (PCP) Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix after sampling via Oral-Eze collection devices, prior to UPLC-MS/MS analysis using ISOLUTE SLE+ supported liquid extraction columns. Estimated LOQs of between 0.005 and 0.75 ng/mL (analyte dependant) are achieved,.

2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Application Notes Basic Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clinical Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oral fluid Over the counter pharmaceuticals Oxazepam Oxycodone Oxymorphone Pain Management Pharmaceuticals Phencyclidine (PCP) Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. High, reproducible drug recoveries are achieved, with sub ng/mL LOQs for most analytes. The method is easily automated using Biotage® Extrahera.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 2-Hydroxyethylflurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Extrahera Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam Lysergic acid diethylamide (LSD) MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oxazepam Oxycodone Oxymorphone Pain Management Phencyclidine (PCP) THC metabolites THC-COOH Temazepam Triazolam Whole blood Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

Drinking water is a significant source of environmental exposure, especially for small children. Countries around the world have put regulations in place to monitor drinking water quality for a wide range of hazardous compounds. Methods such as SL 392-2007 in China, the EN methods in Europe and US methods such as method 525.2 cover a large suite of analytes of concern. They can be effectively extracted using solid phase extraction (SPE) disks and using GC/MS for detection.

5000 Application Notes Chinese Method SL-392-2007 Disk Drinking water English Environmental GC-MS Semi-Volatile Organics

US EPA method 525.2 may use solid phase extraction (SPE) to extract the analytes of interest from water samples. It includes a variety of quality control measures to ensure the method is under control throughout the analysis. The Biotage® Horizon 5000 was used in this study to extract the EPA Method 525.2 analytes from six prepared water samples. The Atlantic high-capacity C18 disks provided excellent recovery of the large suite of compounds extracted in water. The Biotage® Horizon 5000 system provided uniform performance and a hands-off approach to the extraction step. The reproducibility of the six runs was excellent and the average of the standard deviation values was 2.57%.

5000 Application Notes Chinese Method SL-392-2007 Disk Drinking water EPA 525.2 Analytes English Environmental GC-MS US EPA Method 525.2

This application note describes the use of the ISOLUTE® PLD+ Protein and Phospholipid Removal Plate for clean-up of a range of acidic, basic and neutral drugs from plasma. ISOLUTE® PLD+ Protein and Phospholipid Removal Plates are suitable for clean-up of a range of analytes with widely differing functionality and polarity characteristics from plasma, giving high recoveries, good reproducibility and excellent extract cleanliness.

96-well plate Acetaminophen Acidic Amitriptyline Application Notes Atenolol Basic Bretylium Brompheniramine Clinical English Fluoxetine Forensic Ketoprofen LC-MS/MS Metoprolol Mianserin NSAIDs Naltrexone Neutral Pharma Plasma Procainamide Quinidine Ranitidine Salbutamol Sulindac Tricyclic Antidepressant p-toluamide β-blockers

This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant amphetamines followed by derivatization to optimize for GC-MS analysis. Analyte recoveries achieved using this method ranged from 99-104% with RSDs below 10% for all analytes. Amphetamines, SLE, Supported Liquid Extraction, SLE+, ISOLUTE, Urine, GC, GC-MS,

Amphetamine Application Notes Column Drugs of Abuse Forensic GC-MS MDEA MDMA Methamphetamine Urine

Immunosuppressants inhibit or prevent the activity of the immune system used to prevent rejection of transplanted organs and treat autoimmune diseases. Due to the risks of immunodeficiency, those under treatment must undergo constant therapeutic drug monitoring (TDM) requiring reliable and robust analytical techniques for quantification of these drugs. Current methodologies use immunoassay techniques to measure immunosuppressant levels in patients which are expensive, time consuming and susceptible to issues with cross reactivity. The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%. Immunosuppressant, Tacrolimus, Sacrolimus, Everolimus, Cyclosporin A, Clinical, SLE+, SLE, Supported Liquid Extraction, immune, Clinical, Whole Blood, immunoassay,

Application Notes Clinical Cyclosporin A Everolimus Immunosuppressant LC-MS/MS Sacrolimus Sirolimus Tacrolimus Whole blood

This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites opiates, sle, urine, GC-MS, forensic, drugs of abuse, DOA, UCT, agilent

6-acetylmorphine (MAM) Application Notes Codeine Column Dihydrocodeine (DHC) Drugs of Abuse Forensic GC-MS Hydrocodone Hydromorphone Methadone Morphine Oxycodone Oxymorphone Urine

This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application achieves high reproducible recoveries and excellent linearity (>0.99) in the range 10-200 ng/mL.

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Application Notes Clinical English Homovanillic acid (HVA) LC-MS/MS Plasma Vanillylmandelic acid (VMA)

This application note describes a supported liquid extraction protocol for the extraction of three acidic catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application note achieves high reproducible recoveries (>80%, RSD < 5%) for these analytes in plasma with linearity > 0.995 in the range 2–200 ng/mL.

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Application Notes Clinical English Homovanillic acid (HVA) LC-MS/MS Plasma Vanillylmandelic acid (VMA)

This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites ISOLUTE, unknown screening,

Acidic drugs Application Notes Basic drugs Column Forensic Naphyrone Post-Mortem fluids Post-Mortem tissue

The method addresses the extraction of all three classes of drugs of abuse from urine using non-polar and cation exchange retention mechanisms on the ISOLUTE HCX mixed mode column. This protocol was optimized to eliminate the use of dichloromethane in the elution of the acidic / neutral drug fraction.

Acidic drugs Amphetamine Application Notes Basic drugs Benzodiazepines Benzoylecgonine Cocaines Codeine Column Diazepam Forensic Morphine Naphyrone Norcocaine Nordiazepam Opiates Oxazepam THC-COOH Temazepam Urine

This poster demonstrates a novel, sensitive, cost effective and rugged method for the analysis of acrylamide in coffee and potato chips (crisps) using supported liquid extraction prior to LC-MS/MS analysis. It demonstrates extraction from coffee down to a concentration of 1 ng/mL and in potato chips to a level of 10 ng/g (ppb). ASMS 2014

Acrylamide Coffee Column English Food Food contaminant LC-MS/MS Posters Potato chips (crisps)