Part No: P102Issued year: 2014File size: 0.6mbFile type: pdf
Designed polymers are a class of selective resins with engineered selectivities for particular target molecules or ‘classes’ of molecules. These designed polymers are obtained by careful tuning of their surface chemistry and morphology which allows them to exhibit unique separation capabilities. The tailored and optimized selectivity of designed polymers is utilized to conduct difficult separations that are not able to be accomplished with conventional separation resins or other techniques.
Part No: AN933Issued year: 2020File size: 2.25mbFile type: pdf
This application note describes the extraction of a panel of estrogenic hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 5% for all analytes. Linearity of greater than 0.99 is achieved for Estrone (E1) and Estradiol (E2) from 5–2000 pg/mL, and 50–20000 pg/mL for Estriol (E3). No derivatization is required, and detection limits are enhanced using a fluorinated mobile phase.
Part No: P216.V.2Issued year: 2020File size: 2.46mbFile type: pdf
Monitoring estrogenic compounds such as Estrone (E1), Estradiol
(E2), and Estriol (E3) in human serum is essential in clinical research and diagnostics. Detection in men, post-menopausal women and children requires very low limits of detection (LODs) which can be challenging.
Part No: AN927Issued year: 2019File size: 1.18mbFile type: pdf
This application note demonstrates the performance of the
Biotage® Horizon 5000 automated solid phase extraction system
for extraction of 1,4-dioxane from drinking water, in compliance with US EPA Method 522.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: AN053-HOR.V.1Issued year: 2020File size: 1.76mbFile type: pdf
Carbaryl, a commonly used insecticide for the past 20 years, has become a significant environmental concern throughout the world. Also known through its trademarked name Sevin, it is a cholinesterase inhibitor and can be toxic to humans, causing problems in the blood, nervous, and reproductive systems.
Part No: AN089-HORIssued year: 2014File size: 2.48mbFile type: pdf
Deoxynivalenol is a common mycotoxin found in agricultural grain crops and final consumer processed products. Most impacted are wheat, barley, and corn. Deoxynivalenol, also known as Vomitoxin, is produced by Fusarium fungi and has a unique dual-stage growth cycle of producing mold during warm daylight and toxin during cool nights. There is no known procedure or processing that will remove deoxynivalenol that is already present in grain.
Part No: AN001-HOR.V.1Issued year: 2020File size: 1.85mbFile type: pdf
Hormones in drinking water are a growing concern, since the presence of these compounds can potentially be linked to serious health hazards such as human developmental and reproductive side-effects. Hormones are introduced into our environment in several ways. Natural and synthetic hormones that are given to livestock, pass though the animals, runoff into surface water, and leach into underground water supplies. Humans also produce and excrete natural hormone waste every day.
Part No: AN012-HORIssued year: 2010File size: 0.99mbFile type: pdf
The second Unregulated Contaminant Monitoring Regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 529 is categorized under List 1, Assessment Monitoring in the UCMR2 program, and focuses on three explosives: 1,3-dinitrobenzene; 2,4,6- trinitrotoluene (TNT); and hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX). The resulting data will be used by the EPA to determine whether or not regulatory standards should be established for these compounds.
Part No: AN057-HORIssued year: 2010File size: 0.72mbFile type: pdf
At present, pollution of freshwater algae has become aglobal environmental problem. Of all the different pollution types, microcystin LR is the most toxic and the most acute hazard as far as is known presently
Part No: AN078-HORIssued year: 2015File size: 1.25mbFile type: pdf
This application note was developed to demonstrate the extraction of five organophosphate compounds Monocrotophos, Diazinon, Malathion, EPN, and Methamidophos using one solid phase extraction method with one pre-treatment step of sodium chloride (NaCl). The method uses the Biotage® Horizon 4790 automated SPE extraction system. It will show the efficiency of the extraction while demonstrating excellent recoveries of OPP compounds using methylene chloride and minimal amounts of acetone after sample pre-treatment with sodium chloride. Methods were developed and results are shown using 47 mm Atlantic® HLB-H disks and carbon cartridges.
Part No: P201Issued year: 2019File size: 0.9mbFile type: pdf
An extraction protocol using a 10 mg mixed-mode cation exchange sorbent (EVOLUTE® EXPRESS CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving microelution off of the sorbent requiring no evaporation or reconstitution steps were also developed.
Part No: AN009-HOR.V.1Issued year: 2015File size: 0.58mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN108-HORIssued year: 2016File size: 2.35mbFile type: pdf
Drinking water is a critical resource important for human health. As water becomes more scarce, quality will become increasingly important and monitoring will be required more frequently and at lower concentration levels. The number of compounds and lower concentrations for monitoring will make extraction and analysis even more challenging.