In the world of HPLC, loading capacity isn’t normally a concern as it is primarily an analytical technique. In the synthetic organic chemistry world, most purification is performed with silica gel where flash column purification methods are developed and loading capacity estimated from TLC data. However, when normal-phase flash does not work and reversed-phase flash is needed, the question of how to determine reversed-phase loading capacity comes up.
A blog post on the Flash Purification Blog provides some guidelines to help understand and determine reversed-phase flash chromatography loading capacity.
Smarter Workflow Facilitates New Drug Discovery
New Flash Purification System and Columns from Biotage
Speed Up Your Peptide Synthesis Workflow
New Application Note for Simultaneous Extraction of Aldosterone and Angiotensin I and II from Human Plasma
Get Rid of Peptide Cleavage Cocktail in Three Minutes