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Clinical, Forensic and Doping Testing

Automating sample preparation methods such as solid-phase and supported liquid extraction supports high data quality generation and saves ...

Small Molecules and Synthetic Therapeutics

This post discusses the challenges of purifying organic amines and some suggested alternative chromatography techniques.

Small Molecules and Synthetic Therapeutics

Purifying ionic compounds by flash column chromatography.

Small Molecules and Synthetic Therapeutics

This post discusses the value that adding/switching solvents can bring to challenging separations.

Small Molecules and Synthetic Therapeutics

Which sorbents work best for dry loading flash column chromatography samples?

Small Molecules and Synthetic Therapeutics

Determining solvent strength in flash column chromatography is important as it impacts separation effectiveness and fraction purity.

Small Molecules and Synthetic Therapeutics

This post describes the mechanisms behind reversed-phase flash chromatography and where it is most beneficial.

Small Molecules and Synthetic Therapeutics

This post discusses the comparison of linear and step gradients.

Small Molecules and Synthetic Therapeutics

Reversed-phase flash chromatography is growing in popularity. Increasingly, organic and medicinal chemists are turning to reversed-phase to ...

Small Molecules and Synthetic Therapeutics

This post shows data comparing reversed-phase flash chromatography performance vs. prep HPLC.

Small Molecules and Synthetic Therapeutics

Which loading method should I use for purification by flash chromatography?

Small Molecules and Synthetic Therapeutics

This post discusses the benefits of catch & release dry loading using an ion exchange media.

Small Molecules and Synthetic Therapeutics

How to Optimize TLC to Enhance Purification by Flash Chromatography.

Clinical, Forensic and Doping Testing

Underloading your SLE+ samples can result in better partitioning and cleaner samples.

Clinical, Forensic and Doping Testing

Advancements in the Next Generation TurboVap solvent evaporators simplify sample prep and increase sample throughput.

Water and Environmental Testing

One way to prevent crippling the sample throughput in your laboratory from untimely downtime is to consider using a sample cleanup method.

Water and Environmental Testing

What are peak asymmetry requirements for PFAS analysis using EPA Method 537.1?

Biologics and Advanced Therapeutics

Learn more about high-throughput protein purification strategies!

Small Molecules and Synthetic Therapeutics

The article described the impact of introducing the Biotage Peptide Workflow in a university laboratory in terms of reducing the time of ...

Small Molecules and Synthetic Therapeutics

Sometimes synthesizing a peptide doesn't work as well as expected. Read more for suggestions to improve your crude purity and yield.

Small Molecules and Synthetic Therapeutics

Our seasoned peptide chemist Dr. Elizabeth Denton has tried it all when it comes to peptides. In this short video she shows that using DMSO ...

Small Molecules and Synthetic Therapeutics

Using evaporation tool V-10 Touch for peptide chemistry.

Small Molecules and Synthetic Therapeutics

Learn about strategies to prevent methionine oxidation and reduce methionine sulfoxide back to native methionine.

Small Molecules and Synthetic Therapeutics

This video explains how the peptide workflow works according to Biotage.

Small Molecules and Synthetic Therapeutics

A strategy for reducing the time spent weighing out and dissolving amino acids is to prepare a stock solution.

Small Molecules and Synthetic Therapeutics

Synthesizing a library of peptides can be daunting. Read more to learn how double coupling with different coupling reagents can improve ...

Small Molecules and Synthetic Therapeutics

Read more to learn how to improve purification efficiency, both recovery and purity, when crude peptide samples purified as a suspension.

Small Molecules and Synthetic Therapeutics

A series of short videos explaining the main features and benefits of Biotage(R) V-10 Touch, a system for evaporation to remove high ...

Small Molecules and Synthetic Therapeutics

This series of videos show how flexible and efficient the V-10 Touch evaporation system is

Small Molecules and Synthetic Therapeutics

This article illustrate all the benefits of V-10 Touch in removing solvents, also the high boiling point ones, from your compounds and ...

Small Molecules and Synthetic Therapeutics

This post discussed when an ELSD should be used with flash chromatography.

Small Molecules and Synthetic Therapeutics

This post shows flash purification scalability with a real synthetic reaction.

Small Molecules and Synthetic Therapeutics

This post shows the green benefits achieved using an orthogonal purification approach with a highly complex reaction mixture.

Small Molecules and Synthetic Therapeutics

This post discusses the impact reaction temperature has on the yield and purity of the synthetic product.

Small Molecules and Synthetic Therapeutics

This post discusses alternatives to traditional reaction mixture work-up technologies - separatory funnels and rotary evaporators - Biotage ...

Small Molecules and Synthetic Therapeutics

This post discusses the typical steps used by chemists when synthesizing new compounds.

Small Molecules and Synthetic Therapeutics

This post discusses a more efficient extraction methodology for reaction mixtures using recyclable phase separators.

Small Molecules and Synthetic Therapeutics

This post shows that water is a suitable synthesis solvent, enabling greener chemistry.

Small Molecules and Synthetic Therapeutics

This post discusses if the order in which reaction components are added impacts the synthetic product's purity and/or yield.

Small Molecules and Synthetic Therapeutics

This post discusses the impact of time on a reaction.

Small Molecules and Synthetic Therapeutics

This post discusses the impact that reagent concentration has on a synthetic product yield and purity.

Small Molecules and Synthetic Therapeutics

How much does it cost? One of the first questions when considering an automated flash column chromatography system. Here we answer the ...

Small Molecules and Synthetic Therapeutics

Occassionally, peptide chemists still have to couple the first amino acid onto Wang-type resins. Read more to learn how to quantify the ...

Small Molecules and Synthetic Therapeutics

This post shows how gradient slope optimization increases sample loading.

Small Molecules and Synthetic Therapeutics

This post describes various flash chromatography options available for targeted compound purification.

Small Molecules and Synthetic Therapeutics

This post discusses the benefits of purifying the full precipitated reaction mix vs. filtration, phase separation, redissolution and ...

Small Molecules and Synthetic Therapeutics

This post describes alternatives to DCM/Methanol for polar compound purification.

Small Molecules and Synthetic Therapeutics

This post compares the purification results of 5 dry load media vs. liquid loading.

Small Molecules and Synthetic Therapeutics

This post describes methodology to determine reversed-phase column loading capacity.

Small Molecules and Synthetic Therapeutics

Has your chromatogram changed dramatically when you increase sample load? Read more to learn about factors to consider for peptide ...

Small Molecules and Synthetic Therapeutics

Method development for peptide purification can be time consuming, especially when the guess-and-check method is your only option. Improve ...

Small Molecules and Synthetic Therapeutics

Read our blog to learn more about one protection strategy that can be used for these compounds.

Small Molecules and Synthetic Therapeutics

Green chemistry solutions are highly desirable. Read more to learn about greener solvent alternatives to DMF and NMP for peptide synthesis.

Small Molecules and Synthetic Therapeutics

Flow rate affects both selectivity and resolution. Read more to help determine which flow rate works best for purifying peptides with flash ...

Small Molecules and Synthetic Therapeutics

Amino acids can account for a significant fraction of the total cost for peptide synthesis. Read more to learn how many equivalents you can ...

Small Molecules and Synthetic Therapeutics

Fully automating synthesis and secondary chemistry is always desirable, unless it comes at a cost of yield or purity. Read more for ...

Small Molecules and Synthetic Therapeutics

Particle size and pore size can both impact resolution, but especially when using reversed phase flash chromatography. Read more to see ...

Small Molecules and Synthetic Therapeutics

The articles contains an interview to Richard E. Johnsson, Ph.D., Head of Peptide Chemistry at Red Glead Discovery, Lund, Sweden, who ...

Small Molecules and Synthetic Therapeutics

The number of equivalents used in a synthesis can significantly impact the cost of a peptide synthesis. Read more to learn how few ...

Small Molecules and Synthetic Therapeutics

Aspartimide rearrangements can negatively impact biological screening outcomes. Read more to learn methods to identify this side reaction ...

Small Molecules and Synthetic Therapeutics

Aspartimide rearrangements are one of the most ubiquitous side reactions encountered during solid phase synthesis. Read more to learn how ...

Small Molecules and Synthetic Therapeutics

Choosing a stationary phase seems relatively straightforward, almost automatic even. See how selectivity differences could impact your ...

Small Molecules and Synthetic Therapeutics

Sometimes closely eluting impurities are difficult to resolve by simply changing the gradient. Learn how ion pairing agents can help.

Small Molecules and Synthetic Therapeutics

Mass directed purification simplifies purification efforts. Read more how it can be used to reduce your purification time with flash ...

Small Molecules and Synthetic Therapeutics

In this blog, Beth highlights the role of of scavengers for peptides containing cysteine residues.

Small Molecules and Synthetic Therapeutics

Everyone asks about recovery when considering new strategies for peptide purification. Read more to learn about recovery when using flash ...

Small Molecules and Synthetic Therapeutics

Learn how acidic modifier concentration can impact your peptide purification with flash chromatography.

Small Molecules and Synthetic Therapeutics

Mobile phase modifier choice can impact selectivity of peptides for the stationary phase. Read more about using a buffer to modify your ...

Small Molecules and Synthetic Therapeutics

The pH of a peptide isolectric point is a useful reference when evaluating how to handle these compounds.

Clinical, Forensic and Doping Testing

High quality sample prep is critical for preparing samples for analysis and protecting high performance instrumentation in analytical labs.

Clinical, Forensic and Doping Testing

Automating sample preparation methods such as solid-phase and supported liquid extraction with Biotage Extrahera boosts efficiency in the ...

Clinical, Forensic and Doping Testing

Automating sample preparation methods such as solid-phase and supported liquid extraction with Biotage Extrahera boosts efficiency in the ...

Clinical, Forensic and Doping Testing

If you are going to be developing a comprehensive synthetic drug assay, we have outlined the extraction options appropriate for biological ...

Clinical, Forensic and Doping Testing

For fentanyl compounds, there are a few extraction mechanisms that we can use, including supported liquid extraction, Dual Mode extraction ...

Clinical, Forensic and Doping Testing

Choose the right extraction technique for your sample, between Supported Liquid Extraction and Solid Phase Extraction.

Clinical, Forensic and Doping Testing

Understanding the chemical properties of the compounds in your sample matrix, is necessary for successful method development in Sample prep.

Clinical, Forensic and Doping Testing

This blog describes different enzymes and their hydrolysis efficiency for commonly glucuronidated drugs of abuse.

Clinical, Forensic and Doping Testing

This blog discusses advantages and optimization of the in-well hydrolysis technique.

Clinical, Forensic and Doping Testing

The blog contains a step by step guide to develop a Supported Liquid Extraction method in 16 steps.

Clinical, Forensic and Doping Testing

Learn more about how Supported Liquid Extraction works and the proper way to do an SLE extraction for sample clean up.

Clinical, Forensic and Doping Testing

This blog is devoted to the mathematical relationship between the universally accepted criteria for method robustness and assay confidence.

Clinical, Forensic and Doping Testing

Using the “pH rule of 2” and knowing the pH at which your analytes are charged can produce cleaner samples by using mixed mode ion exchange ...

Clinical, Forensic and Doping Testing

This post explains the differences between anionic and cationic SPE exchange phases and how optimize an extraction protocol using them.

Clinical, Forensic and Doping Testing

SPE of strong acids and bases is done by mixed mode weak ion exchange, using pH to change the charge state of the sorbent instead of the ...

Clinical, Forensic and Doping Testing

This blog introduces the difference between SPE and SLE.

Clinical, Forensic and Doping Testing

This blog gives some guidelines to help you to choose the most appropriate SPE ion exchange media.

Clinical, Forensic and Doping Testing

This blog gives useful insights to learn how to monitor and prevent sample carryover during an analysis method development.

Clinical, Forensic and Doping Testing

This blog helps to understand hot to modify a sample preparation method optimized for LC/MS-MS analysis to better fit for a GC-MS analysis.

Clinical, Forensic and Doping Testing

This blog helps you determine the optimal sample volume for a sorbent bed mass and helps you getting recovery for all compounds in your ...

Clinical, Forensic and Doping Testing

This blog discusses the advantages and disadvantages of vacuum pressure and positive pressure extraction.

Clinical, Forensic and Doping Testing

This blog provide some tips about how to disrupt protein binding with sample preparation.

Clinical, Forensic and Doping Testing

How to develop extraction methods for persistent contaminants like nicotine, cotinine and tobacco related compounds and metabolites in ...

Clinical, Forensic and Doping Testing

This blog post helps troubleshooting when you are not achieving complete frit coverage on the load of your sample.

Clinical, Forensic and Doping Testing

This blog describes advantages and disadvantages of the analysis of large panel of drugs that contains compounds from multiple drug classes.

Clinical, Forensic and Doping Testing

This blog helps to understand the advantages of filtering your samples instead of dilute and shoot into your LC-MS/MS.

Clinical, Forensic and Doping Testing

Rebecca Mastrovito from NMS has simplified the extraction of 12 novel synthetic cannabinoids using the Biotage ISOLUTE SLE+ cartridges.

Clinical, Forensic and Doping Testing

This blog helps developing analytical methods providing list of reference websites for literature research related to analytes of interest.

Clinical, Forensic and Doping Testing

This blog provide tips on how to find reliable sources of information related to your analytes of interest before developing a new ...

Clinical, Forensic and Doping Testing

This blog post explains the how to ensure your analytes reach the low vacuum stage ion optics of the mass spectrometer in the greatest ...

Clinical, Forensic and Doping Testing

The ACT Adapter reduces crosstalk of volatile drugs like amphetamines which can cause false positive results and re-extraction of clinical ...

Clinical, Forensic and Doping Testing

This blog explains the first steps on developing a new method for sample preparation and suggest helping tools.

Clinical, Forensic and Doping Testing

This blog gives some advise to resolve many issues commonly encountered with mass analyzers, specifically, LC-MS/MS systems.

Small Molecules and Synthetic Therapeutics

Purifiying hydrophilic peptides present a unique challenge for reversed phase chromatography. Read more how injection solvent can impact ...

Small Molecules and Synthetic Therapeutics

The overall hydrophobicity of a peptide can severely limit the synthesis success. Read more to learn how choosing an alternative system ...

Small Molecules and Synthetic Therapeutics

Purifying synthetic peptides can be challenging and time consuming. Read more to learn how the solvent chosen to dissolve peptides can ...

Clinical, Forensic and Doping Testing

This blogs describes how to do systematic sample prep method development for clinical LC-MS/MS assays.

Clinical, Forensic and Doping Testing

Comparison of two sample prep techniques: protein precipitation vs. traditional protein crash.

Clinical, Forensic and Doping Testing

This blog helps understanding the difference among SLE and SPE.

Clinical, Forensic and Doping Testing

Discover how different sample preparation techniques can help you reach your low detection limit targets.

Clinical, Forensic and Doping Testing

Find out how microelution SPE works to increase analytical sensitivity without the need for evaporation.

Clinical, Forensic and Doping Testing

Discover how to improve throughput and increase analyte sensitivity using microelution SPE.

Clinical, Forensic and Doping Testing

This blog provides insightful information to help cleanup urine samples prior analysis.

Clinical, Forensic and Doping Testing

This blog post identifies and explains the merit behind aligning the mass spectrometer’s optics to enhance analyte identification and ...

Clinical, Forensic and Doping Testing

This blog helps diagnose and successfully troubleshoot issues surrounding signal loss during method development of large analytes panels ...

Clinical, Forensic and Doping Testing

This blog helps developing a method for the extraction of a panel of vitamins A, E and K.

Clinical, Forensic and Doping Testing

Biotage® Mikro SPE plates require slightly higher processing pressures due to the unique high-performance design.

Clinical, Forensic and Doping Testing

When looking at developing or modifying a LC/MS or GC/MS assay, one of the most important steps is the sample prep.

Clinical, Forensic and Doping Testing

What are the challenges when developing new laboratory methods.

Clinical, Forensic and Doping Testing

Developing a new sample prep method should be systematic to ensure ruggedness, and can be done quickly with the right tools.

Clinical, Forensic and Doping Testing

Correct pretreatment of samples prior to supported liquid extraction (SLE) produces better partitioning and cleaner extracts.

Clinical, Forensic and Doping Testing

Choosing the SLE correct elution solvent based on analyte properties produces the cleanest extracts with the highest recoveries.

Clinical, Forensic and Doping Testing

We can now program and store methods on the Next Generation TurboVap solvent evaporators to ensure reproducibility from run to run.

Water and Environmental Testing

Thermistors are most useful, at least from my perspective, when they’re used in automated solid-phase extraction systems.

Water and Environmental Testing

Phenolic compounds can be some of the most challenging compounds to extract from the compound lists in EPA Method 8270 and EPA Method 625.1

Water and Environmental Testing

Processing samples in an EPA-regulated laboratory, you know that these methods can be very specific in some spots, and incredibly vague in ...

Water and Environmental Testing

In this blog post we answers questions about TurboVap® evaporators and their use in an environmental lab.

Water and Environmental Testing

Common mistakes and good lab practices in the analytical testing lab.

Small Molecules and Synthetic Therapeutics

Getting started with a new technique can be challenging and even frustrating. Read more for tips and tricks as you get started using flash ...

Small Molecules and Synthetic Therapeutics

The complexity of synthetic peptides continually increases. Read more to learn how microwave heating can improve your crude synthetic ...

Small Molecules and Synthetic Therapeutics

Fractionating using multiple wavelengths simultaneously improves compound purity and yield compared to using one or two individual ...

Small Molecules and Synthetic Therapeutics

Removing a co-eluting impurity requires changing the purification method. Usually done with solvent change here we show the results of ...

Small Molecules and Synthetic Therapeutics

Wang resin is available preloaded with many amino acids, but there may be times you have to perform the chemistry yourself. Read more to ...

Small Molecules and Synthetic Therapeutics

Fully protected peptides can be difficult to purify using standard reversed-phase methods. Learn about normal phase chromatography as an ...

Water and Environmental Testing

EPA method 525.2 has been one of the most widely used methods for quantifying semi-volatile compounds in drinking water.

Water and Environmental Testing

What are the differences between EPA Method 533 and 537.1? Our blog reviews each method and highlights key differences between each.

Water and Environmental Testing

If you’re a lab processing drinking water samples using solid phase extraction, you find yourself saying “How do I dry my extracts?”

Water and Environmental Testing

This blog post focus is on the changes the EPA has made to Method 525 which affect the analysis of the prepared samples.

Water and Environmental Testing

The U.S. EPA monitors a variety of compounds that pose public health risks when they are present in our air, soil or water.

Water and Environmental Testing

Pesticides are chemicals that are sprayed onto our crops, eventually ending up in our food and water supply.

Water and Environmental Testing

Read our technical tips on how to deal with vapor lock in automated solid phase extraction.

Small Molecules and Synthetic Therapeutics

There are many available resins for solid phase peptide synthesis. Read more to learn about some of the choices and how they differ from ...

Small Molecules and Synthetic Therapeutics

In this post, we'll put to use three orthogonal protection strategies to optimize a fully automated synthesis of linaclotide.