Automated extraction of Zolmitriptan from plasma using ISOLUTE® SLE+ with Biotage® Extrahera™ Classic prior to UHPLC-MS/MS analysis

This application note demonstrates the ISOLUTE® SLE+ automation workflow to process plasma samples prior to LC-MS/MS Zolmitriptan (figure 1), a selective serotonin receptor agonist, was spiked into plasma to mimic the biological samples for PK/PD study and used to evaluate the performance of the workflow. The Biotage® Extrahera™ Classic sample preparation workstation was used to automatically perform all the extraction steps. Our results indicated that this automated workflow offers high analyte recoveries, minimized matrix effect, excellent intra and inter plate precision and accuracy, and significantly reduced bench labor time.