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    Documents | The Effect of Wetting and Equilibrating C18 RP Columns at Higher Flowrate and Pressure in Flash Chromatography

    The Effect of Wetting and Equilibrating C18 RP Columns at Higher Flowrate and Pressure in Flash Chromatography

    Wetting and equilibration of C18 columns for flash chromatography are best performed at the highest possible pressures/ flowrates in order to remove the air trapped inside the porous material. That will make more C18-sites activated and accessible during the chromatography run.

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